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Preparing method of compound microorganism agent for papermaking wastewater

A technology of composite microbial bacterial agent and papermaking wastewater, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as dark black liquor, polluted water sources, health and environmental hazards, and achieve promising prospects Effect

Inactive Publication Date: 2015-08-12
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Papermaking wastewater is further divided into pulp and papermaking wastewater, papermaking wastewater in the middle stage of pulp washing and bleaching, and white water generated in the papermaking process. Pulp and papermaking wastewater is commonly known as black liquor, which contains a large amount of cellulose, pigment, lignin and inorganic salts; However, there are a large amount of cellulose and fillers in the middle water and white water. The black liquor in the papermaking wastewater is the most harmful, and its pollutants account for more than 90% of the total pollution discharge of the papermaking industry. Because the black liquor is dark in color and alkaline Big, foamy, smelly, and consume a lot of dissolved oxygen in water, seriously pollute water sources, and bring harm to human health and the environment. Currently, traditional multimedia filtration, sand filtration, and activated carbon filtration processes are used to reuse wastewater. It can only reduce the concentration of suspended solids in water to a certain extent, and cannot further remove ammonia nitrogen, soluble pollutants, COD and salt in wastewater. The traditional activated sludge method uses activated sludge to treat wastewater, which will be affected The application is limited due to environmental and other factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Step 1. Activate and expand each strain:

[0045] Firstly, Trametes yunzhi, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus and Pseudomonas alcaligenes were activated on the solid medium plate; then, the liquid medium of each strain was added to different In the Erlenmeyer flask, connect the single colonies on each solid medium to the corresponding Erlenmeyer flask, and cultivate at 28-30℃ until the bacterial cell content in each Erlenmeyer flask reaches 1.0×10 8 -1.0×10 9 Pcs / mL; then add the liquid culture medium of each strain into different fermenters, and connect the bacteria in the triangular flask to the corresponding fermentor, and cultivate the bacteria in each fermentor at 28-30℃ Body content reaches 1.0×10 8 -1.0×10 9 Pcs / mL;

[0046] Step 2. Centrifugal separation and drying to obtain the microbial powder:

[0047] Centrifugal separation of the microbial bacteria liquid obtained by the expansion of the fermenter in step 1, collect the precipitate ...

Embodiment 2

[0051] Step 1. Activate and expand each strain:

[0052] Firstly, Trametes yunzhi, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus and Pseudomonas alcaligenes were activated on the solid medium plate; then, the liquid medium of each strain was added to different In the Erlenmeyer flask, connect the single colonies on each solid medium to the corresponding Erlenmeyer flask, and cultivate at 28-30℃ until the bacterial cell content in each Erlenmeyer flask reaches 1.0×10 8 -1.0×10 9 Pcs / mL; then add the liquid culture medium of each strain into different fermenters, and connect the bacteria in the triangular flask to the corresponding fermentor, and cultivate the bacteria in each fermentor at 28-30℃ Body content reaches 1.0×10 8 -1.0×10 9 Pcs / mL;

[0053] Step 2. Centrifugal separation and drying to obtain the microbial powder:

[0054] After centrifugal separation of the microbial bacteria liquid obtained by the expansion of the fermentor in step 1, the collected sedi...

Embodiment 3

[0058] Step 1. Activate and expand each strain:

[0059] Firstly, Trametes yunzhi, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus and Pseudomonas alcaligenes were activated on the solid medium plate; then, the liquid medium of each strain was added to different In the Erlenmeyer flask, connect the single colonies on each solid medium to the corresponding Erlenmeyer flask, and cultivate at 28-30℃ until the bacterial cell content in each Erlenmeyer flask reaches 1.0×10 8 -1.0×10 9 Pcs / mL; then add the liquid culture medium of each strain into different fermenters, and connect the bacteria in the triangular flask to the corresponding fermentor, and cultivate the bacteria in each fermentor at 28-30℃ Body content reaches 1.0×10 8 -1.0×10 9 Pcs / mL;

[0060] Step 2. Centrifugal separation and drying to obtain the microbial powder:

[0061] After centrifugal separation of the microbial bacteria liquid obtained by the expansion of the fermentor in step 1, the collected sedi...

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PUM

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Abstract

The invention provides a preparing method of a compound microorganism agent for papermaking wastewater. The compound microorganism agent is prepared by mixing Trametes versicolor, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus, Pseudomonas alcaligenes and cellulase; the cellulase can decompose celluloses in the wastewater into glucose; compound microorganism can also degrade solids, lignin and the like in the wastewater. The compound microorganism agent is free of chemicals, environment friendly, non-toxic and free of secondary pollution, is simple to use, is insusceptible to environmental factors, has stable effect on wastewater treatment and allows the quality of effluent to meet the national standard.

Description

Technical field [0001] The invention relates to the technical field of sewage treatment, in particular to the preparation of a composite microbial agent for papermaking wastewater. Background technique [0002] Wastewater in the paper industry is a kind of difficult-to-treat organic wastewater with large water volume, large suspended solids content, high color and high organic concentration. The raw materials used are mainly straw, wood, reeds, and rags, which are separated after high temperature and high pressure cooking. Cellulose, made into pulp. [0003] Papermaking wastewater is divided into pulp and papermaking wastewater, pulp washing and bleaching mid-stage papermaking wastewater and white water generated in the papermaking process. Pulp and papermaking wastewater is commonly known as black liquor, which contains a large amount of cellulose, pigments, lignin and inorganic salts; In the middle water and white water, there are a lot of cellulose and fillers. The black liquor...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/125C12R1/08C12R1/38C12R1/645C02F103/28
CPCC12N1/20C02F3/347C02F2103/28C12N1/14
Inventor 龚国利张甜
Owner SHAANXI UNIV OF SCI & TECH
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