Compound microorganism microsphere and preparation method thereof
A technology of composite microorganisms and microspheres, which is applied in the field of composite microbial microspheres and its preparation, can solve the problems of high equipment costs, maintenance and operation costs, secondary pollution, and difficulty in popularization, and achieve enhanced cross-linking, improved efficiency, fixed firm effect
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Embodiment 1
[0023] Preparation of embedding carrier mixture: Add 40.5g polyvinyl alcohol (PVA) and 4.05g sodium alginate into 300mL sterile deionized water, heat to dissolve in a water bath at 90°C, and cool to room temperature (25°C).
[0024] Preparation of boric acid-calcium chloride solution: first prepare saturated boric acid solution, add 2g of calcium chloride per liter of saturated boric acid solution, after fully dissolved, adjust the pH of the solution to 6.5-7.0 with sodium bicarbonate solution.
[0025] A preparation method of composite microbial microspheres, comprising the following steps:
[0026] 1) Inoculate activated Pseudomonas mendoza and Pseudomonas pseudoalcaligenes in LB liquid medium respectively, culture at 30°C and 150r / min for 24h, and centrifuge at 5000r / min respectively After 10 min, the supernatant was discarded to obtain the thallus, and after washing the thallus twice with sterile deionized water, they were mixed at a weight ratio of 1: 1 to prepare composi...
Embodiment 2
[0029] Embodiment 2 effect test
[0030] 500ml artificially prepared mass concentration of 100mg / l ammonia nitrogen sewage and nitrate nitrogen sewage were respectively placed in conical flasks, marked as test group 1 and test group 2, and 100 composite microorganisms prepared in Example 1 were taken The microspheres were placed in the Erlenmeyer flasks of test group 1 and test group 2 respectively, and cultured at 25°C for 120 h at a rotational speed of 100 r / min. During the culture period, the culture solution was regularly taken and centrifuged at 8000 r / min for 5 min, and the supernatant was taken respectively. liquid, the ammonia nitrogen and nitrate nitrogen in the supernatant were detected and analyzed, and the results were as follows in Table 1:
[0031]
[0032] As can be seen from Table 1, the composite microbial microspheres of the present invention have a better removal effect on ammonia nitrogen and nitrate nitrogen in sewage, especially better on the removal e...
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