Compound microorganism microsphere and preparation method thereof

A technology of composite microorganisms and microspheres, which is applied in the field of composite microbial microspheres and its preparation, can solve the problems of high equipment costs, maintenance and operation costs, secondary pollution, and difficulty in popularization, and achieve enhanced cross-linking, improved efficiency, fixed firm effect

Active Publication Date: 2016-11-09
河南永泽环境科技有限公司
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  • Application Information

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Problems solved by technology

[0002] At present, the methods of removing nitrogen in sewage include physical and chemical methods and biological methods. etc., have good effluent quality, good nitrogen and phosphorus removal effect, small footprint, and strong adaptability to changes in wastewater temperature, water volume, and concentration. Promotion, and easy to cause secondary pollution

Method used

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  • Compound microorganism microsphere and preparation method thereof
  • Compound microorganism microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of embedding carrier mixture: Add 40.5g polyvinyl alcohol (PVA) and 4.05g sodium alginate into 300mL sterile deionized water, heat to dissolve in a water bath at 90°C, and cool to room temperature (25°C).

[0024] Preparation of boric acid-calcium chloride solution: first prepare saturated boric acid solution, add 2g of calcium chloride per liter of saturated boric acid solution, after fully dissolved, adjust the pH of the solution to 6.5-7.0 with sodium bicarbonate solution.

[0025] A preparation method of composite microbial microspheres, comprising the following steps:

[0026] 1) Inoculate activated Pseudomonas mendoza and Pseudomonas pseudoalcaligenes in LB liquid medium respectively, culture at 30°C and 150r / min for 24h, and centrifuge at 5000r / min respectively After 10 min, the supernatant was discarded to obtain the thallus, and after washing the thallus twice with sterile deionized water, they were mixed at a weight ratio of 1: 1 to prepare composi...

Embodiment 2

[0029] Embodiment 2 effect test

[0030] 500ml artificially prepared mass concentration of 100mg / l ammonia nitrogen sewage and nitrate nitrogen sewage were respectively placed in conical flasks, marked as test group 1 and test group 2, and 100 composite microorganisms prepared in Example 1 were taken The microspheres were placed in the Erlenmeyer flasks of test group 1 and test group 2 respectively, and cultured at 25°C for 120 h at a rotational speed of 100 r / min. During the culture period, the culture solution was regularly taken and centrifuged at 8000 r / min for 5 min, and the supernatant was taken respectively. liquid, the ammonia nitrogen and nitrate nitrogen in the supernatant were detected and analyzed, and the results were as follows in Table 1:

[0031]

[0032] As can be seen from Table 1, the composite microbial microspheres of the present invention have a better removal effect on ammonia nitrogen and nitrate nitrogen in sewage, especially better on the removal e...

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Abstract

The invention discloses a compound microorganism microsphere and a preparation method thereof, and belongs to the technical field of microorganisms in sewage treatment. The method comprises the following steps that (1) pseudomonas mendocina and pseudomonas pseudoalcaligenes are subjected to primary embedding and crosslinked immobilization; (2) the product obtained through primary crosslinked immobilization in the first step and bacillus subtilis are mixed and are then subjected to secondary embedding and crosslinked immobilization; the compound microorganism microsphere is obtained. Two layers of microorganisms are embedded in the compound microorganism microsphere prepared by the preparation method; the inner layer contains the pseudomonas mendocina and the pseudomonas pseudoalcaligenes; the outer layer contains the bacillus subtilis. The embedding method is used twice, so that the microorganism immobilization is firmer; the bacteria density is increased; the sewage treatment efficiency is improved; the magnet powder has strong adsorption force; the crosslinking effect among mixed microorganisms is enhanced; the immobility of mixed bacteria is improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms in sewage treatment, and in particular relates to a composite microbial microsphere and a preparation method thereof. Background technique [0002] At present, the methods for removing nitrogen in sewage include physical and chemical methods and biological methods. Physical and chemical denitrification methods mainly include air stripping method, breakpoint chlorine oxidation method, gas method, crystallization method, chemical precipitation method, ion exchange, electrodialysis and reverse osmosis. etc., have good effluent quality, good nitrogen and phosphorus removal effect, small footprint, and strong adaptability to changes in wastewater temperature, water volume, and concentration. Promotion, and easy to cause secondary pollution. The biological denitrification method can efficiently remove nitrogen in sewage by enzymatic catalysis. It has the advantages of simple removal method, low ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/08C12N11/04C02F3/34C12R1/125C12R1/38C02F101/16
CPCC02F3/341C02F2101/16C12N11/04C12N11/08C12N11/10C12N11/14
Inventor 周明周素莹邢传宏车晓玲
Owner 河南永泽环境科技有限公司
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