36 results about "Pseudomonas pseudoalcaligenes" patented technology

There is provided an enzymatic detergent composition which comprises: (a) surfactant; (b) 10-20,000 LU per gram of the detergent composition of a lipolytic enzyme obtainable from Humicola lanuginosa, Pseudomonas pseudoalcaligenes, Rhizomucor miehei and (c) a non-cross-bridged polydentate N-donor ligand capable of forming a complex with a transition metal, wherein said complex is capable of catalysing the bleaching of stains on fabrics by means of atmospheric oxygen.

The invention discloses efficient mixed flora for treating sludge which comprises comamonas testosteroni, pseudomonas alcaligenes, stenotrophomonas rhizophila, pseudomonas pseudoalcaligenes and azospirillum. The mixed flora can effectively decompose the sludge.

The invention discloses pseudomonas pseudoalcaligenes and application of the pseudomonas pseudoalcaligenes to preparation of a sitagliptin intermediate. A colony of the pseudomonas pseudoalcaligenes XW-40 is flat and round in surface morphology, smooth and neat in edge, moist, milk white and opaque and is about 1.9mm in diameter. The pseudomonas pseudoalcaligenes is capable of effectively catalyzing 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro-[1,2,4]triazolo[4,3-a]pyrazine-7(8H)-yl]-1-(2,4,5)-trifluorophenyl)-butan-2-one to be reduced into a sitagliptin chiral intermediate; in optimal reaction conditions, the pseudomonas pseudoalcaligenes can be tolerant to 10 g / L of high substrate concentration while keeps high selectivity, and is weak in substrate inhibition effect, so that high-substrate-concentration biological catalysis can be achieved; the pseudomonas pseudoalcaligenes is recyclable and removes inhibition effect of a substrate and a product on cells as compared with fed-batch, so that catalytic activity of the pseudomonas pseudoalcaligenes is used maximally.

The invention provides a composite immobilized microbial agent as well as a preparation method and application thereof. The preparation method comprises the following steps of preparing a bacterial suspension of petroleum hydrocarbon degrading bacteria, wherein the petroleum hydrocarbon degrading bacteria comprise one or a combination of more of a pseudomonas pseudoalcaligenes strain, a pseudomonas guguanensis strain, am acinetobacter venetianus and a sphingobacterium pakistanense strain; adding biochar into the bacterial suspension, and uniformly mixing to enable the petroleum hydrocarbon degrading bacteria to be fully adsorbed on the biochar, so as to obtain a biochar-bacterial liquid mixed solution; and uniformly mixing a mixed NaCl aqueous solution of sodium alginate and polyvinyl alcohol with the biochar-bacterial liquid mixed solution to carry out cross-linking immobilization, dropwise adding the obtained mixed solution into a CaCl2-boric acid solution, cleaning the obtained mixed solution with the NaCl aqueous solution, placing the mixed solution on a sterile culture medium plate, and naturally airing. The method is simple and easy to implement, low in cost and high in operability; and the obtained composite immobilized microbial agent has excellent mechanical strength and excellent degradation effect on petroleum hydrocarbon.

The invention relates to a denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof. The strain is named as pseudomonas pseudoalcaligenes CL-1, belongs to pseudomonas pseudoalcaligenes, and is preserved in China Center for Type Culture Collection on June 13, 2012, and the preservation numbers is CCTCC NO: M2012225. The strain is gram-positive and belongs to brevibacterium, a bacterial colony is rounded, milk white, convex and glossy, and edges are smooth and transparent. The growth cycle of the organism is long, the nitrogen and phosphorus removal rates in the first 24h are respectively 56.93% and 43.56%, and the nitrogen and phosphorus removal rates in the whole growth cycle are respectively 75.6% and 57.5%. The strain has a complete denitrifying capacity, and can directly reduce inorganic nitrogen such as nitrate nitrogen and nitrite nitrogen and the like to harmless nitrogen gas discharged from water bodies. When the denitrifying phosphate-accumulating organism is used for treating nitrogen and phosphorus containing wastewater, the process is simple, the treatment effect is efficient and stable, and the operating cost is saved, meanwhile, no greenhouse gas such as NO and N2O and the like is produced, therefore, no air pollution is caused.

The invention provides a bacteria strain having a high efficiency broad spectrum degradation ability for an organophosphorus pesticide, the strain belongs to Pseudomonas pseudoalcaligenes, and is named as C2-1, the invention also provides a seperation process, cultivation conditions, and a degradation characteristic of the strain, the strain can be grew on an inorganic salt culture medium by using the pesticide. Also provided is seperation and purification of an organophosphorus pesticide degradation enzyme excreted by the strain, and study on enzyme properties, and seperation and clone of genes coding the enzyme. The invention provides a good gene source for cloning organophosphorus pesticide degradation enzyme genes by using a gene project means, finally realizes an object of producing the organophosphorus pesticide degradation enzyme.

The invention discloses a method for treating heavy metal wastewater. The method comprises the following steps: 1) immobilizing pseudomonas pseudoalcaligenes and saccharomyces cerevisiae mixed bacteria collected after mixed fermentation culture into immobilized cell spheres of which the appearance is spherical and the interior is of a porous meshed structure; 2) taking starch raw material hydrolysate as a culture medium, inoculating rhizopus oryzae spore suspension under the condition that the pH value is equal to 3-6, performing shaking culture for 24-48 hours, filtering and collecting to obtain rhizopus oryzae mycelium pellets of which the diameter is 50-100 microns; 3) filling the immobilized cell spheres and the rhizopus oryzae mycelium pellets in a biological adsorber, wherein the biological adsorber is formed by connecting a plurality of adsorbing columns in series; 4) regulating the initial heavy metal ion concentration of the heavy metal wastewater to be less than or equal to 800 mg / L, and allowing the wastewater to flow through the biological adsorber after the pH value is equal to 5-7, wherein the hydraulic retention time in each adsorbing column is 0.5-1 hour. By utilization of different types of microbial viable bacteria, different heavy metals (such as Cd<2+>, Hg<2+>, Cu<2+>, Zn<2+>, Ni<2+>, Ag<2+> and Pb<2+>) can be well adsorbed under the effects of different adsorption mechanisms.

The invention relates to a strain of Pseudomonas pseudoalcaligenes (Pseudomonas pseudoalcaligenes) NYJ3.6. The invention also relates to the application of the above bacteria in petroleum degradation and a floating carbon immobilized bacteria agent containing the above NYJ3.6, and provides the preparation method and application of the bacteria agent. The Pseudomonas pseudoalcaligenes NYJ3.6 provided by the present invention has the effect of promoting petroleum degradation, and can be used to solve the problem of petroleum pollution; secondly, the floating carbon immobilized bacteria agent containing the above-mentioned Pseudomonas alcaligenes NYJ3.6, Using 100 mesh above 800°C reed rods as the biochar immobilization carrier, the biochar can adsorb oil more efficiently before its own precipitation. After absorbing oil, its buoyancy can be improved while reducing the surface viscosity of oil, which can Promote oil degradation more effectively.

The invention relates to the field of microbial technology, and particularly provides a microbial agent capable of degrading garbage. Domestic garbage, especially kitchen waste, can be efficiently degraded through the synergetic promoting effect of bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, the degradation rate can achieve 80%, the living environment can be beneficially improved, and the environmental pollution can be alleviated.

The invention discloses pseudomonas pseudoalcaligenes and application of the pseudomonas pseudoalcaligenes to preparation of a sitagliptin intermediate. A colony of the pseudomonas pseudoalcaligenes XW-40 is flat and round in surface morphology, smooth and neat in edge, moist, milk white and opaque and is about 1.9mm in diameter. The pseudomonas pseudoalcaligenes is capable of effectively catalyzing 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro-[1,2,4]triazolo[4,3-a]pyrazine-7(8H)-yl]-1-(2,4,5)-trifluorophenyl)-butan-2-one to be reduced into a sitagliptin chiral intermediate; in optimal reaction conditions, the pseudomonas pseudoalcaligenes can be tolerant to 10 g / L of high substrate concentration while keeps high selectivity, and is weak in substrate inhibition effect, so that high-substrate-concentration biological catalysis can be achieved; the pseudomonas pseudoalcaligenes is recyclable and removes inhibition effect of a substrate and a product on cells as compared with fed-batch, so that catalytic activity of the pseudomonas pseudoalcaligenes is used maximally.

The invention discloses a mixed bacterial colony microbial preparation which is a bacterial suspension consisting of paenibacillus, pseudomonas stutzeri, pseudomonas pseudoalcaligenes and pseudomonas oleovoran, wherein the viable count of paenibacillus is more than 1.69*10<10>CFU / Ml, the viable count of pseudomonas stutzeri is more than 2.35*10<10>CFU / Ml, the viable count of pseudomonas pseudoalcaligenes is more than 2.18*10<10>CFU / Ml, and the viable count of pseudomonas oleovoran is more than 2.26*10<10>CFU / Ml. The mixed bacterial colony microbial preparation can be used for treating wastewater containing nitrate nitrogen, and the specific application method comprises the steps of regulating the pH value of the wastewater containing nitrate nitrogen to 7.0-7.2, then inoculating the wastewater containing nitrate nitrogen with the mixed bacterial colony microbial preparation according to the inoculation amount of 1-10%, and performing static culture at 30-35 DEG C. The denitrification effect can be obvious after 4 days culture and complete denitrification can be realized.

The invention relates to the technical field of microorganisms, and particularly provides a biological treating agent for refuse degradation. According to the biological treating agent, bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and brewer yeasts are used in cooperation with carriers, household refuse, especially, kitchen waste, can be efficiently degraded, the degradation rate reaches 85%, the biological treating agent can be widely applied to refuse centralized treatment, the biological treating agent is favorable for the improvement of the living environment, and pollution is reduced.

The invention relates to a method for producing a microbial agent for processing garbage. The method particularly includes the steps of firstly, conducting high-density liquid fermentation on bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudolcaligenes and sacharomyces cerevisiae; secondly, preparing super-thick microbial powder with the spore content ranging from 109CFU / g to 1010CFU / g through a spray drying method; thirdly, weighing and taking, in proportion, 30 parts to 100 parts of the bacillus cereus prepared in the first step, 100 parts to 300 parts of the lactobacillus acidophilus prepared in the first step, 100 parts to 150 parts of the bacillus stearothermophilus prepared in the first step, 50 parts to 120 parts of the pseudomonas pseudolcaligenes prepared in the first step and 80 parts to 150 parts of the sacharomyces cerevisiae prepared in the first step, and mixing the mixture even in a stirring machine; fourthly, drying the mixed microbial powder in an air drying cylinder to obtain the microbial agent for processing the garbage. The method for producing the microbial agent is simple, suitable for an existing process and existing fermentation devices, and easy to widely popularize.

The present invention provides a kind of new pesticidal protein gene and the gene coded protein. The pesticidal protein gene is originated from Pseudomonas pseudoalcalligenes, and its coded protein has wide and powerful intoxicating effect on grasshopper and Orthoptera insects, so that the pesticidal protein gene may be used in preventing and controlling pest of crop. The present invention also provides the Pseudomonas pseudoalcalligenes pesticidal protein separating and purifying method, and one method of producing pesticidal transgenic plant.