The invention relates to a method of catalytically synthesizing
sucrose-6-acetate by
Rhizomucor miehei lipase. The method is characterized by comprising the steps of a. adding DMF and tert-pentyl
alcohol into a reaction kettle according to a volume ratio of 1:(3 to 5), and performing uniform mixing; b, adding
sucrose into a reaction kettle; adding
vinyl acetate and the
Rhizomucor miehei lipase, controlling the concentration of the
sucrose to be 0.03 to 0.12 mmoL / mL, the concentration of the
Rhizomucor miehei lipase to be 6 to 60 mg / mL, the mol ratio of the sucrose to the
vinyl acetate to be 1:(5 to 20) and the temperature to be 30 to 35 DEG C to react for 8 to 24 h, performing
filtration, and placing filter liquid at a
room temperature for 20 to 40 min; c, performing reduced pressure
distillation on the filter liquid at the temperature of 50 to 70 DEG C and the pressure of -0.080 to -0.1 MPa, cooling liquid in a condensing groove to the
room temperature, adding
dichloromethane, addingthe
dichloromethane again after
turbidity occurs, controlling the ratio of the total quantity of the
dichloromethane to the volume of the condensing liquid to be (4 to 7):3, performing still standingand
filtration to obtain mother liquid; and d, pumping the mother liquid into a centrifugal
machine to obtain faint yellow crystals. The method provided by the invention has the advantages that the
reaction conditions are mild; the operation is simple; the generation of isomers is avoided; the catalytic selectivity, the product safety and the conversion rate are high; and the cost is low.