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A compound microbial agent for efficient degradation of lignocellulosic fibers and its application in composting

A compound bacterial agent and wood fiber technology, applied in the field of agricultural microorganisms, can solve the problems of insufficient degradation, complex composition of livestock and poultry manure and straw, and slow speed, so as to improve the economic benefits of enterprises and the ecological environment, and the synergy effect is obvious. , the effect of improving production efficiency

Active Publication Date: 2020-12-15
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition of livestock and poultry manure and straw is complex, including protein, fat, organic acid, cellulose, hemicellulose, and inorganic salts. At present, relying on a single bacterial species or a simple combination of complex bacterial flora is insufficient and slow to degrade

Method used

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  • A compound microbial agent for efficient degradation of lignocellulosic fibers and its application in composting
  • A compound microbial agent for efficient degradation of lignocellulosic fibers and its application in composting
  • A compound microbial agent for efficient degradation of lignocellulosic fibers and its application in composting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0034]Implementation Case 1 Ability of Single Bacteria and Composite Bacteria to Produce Lignocellulose Degrading Enzymes

[0035] Enzyme activity kits (Suzhou Keming Biotechnology Co., Ltd.) were used to determine the enzyme activities related to the degradation of lignin and cellulose for both single and combined bacteria.

[0036] (1) β-glucosidase (β-GC) activity assay

[0037] β-glucosidase decomposes p-nitrobenzene-β-D-glucopyranoside to generate p-nitrobenzene, which has a maximum absorption peak at 400nm, and calculates β-glucosidase by measuring the rate of increase in absorbance vitality.

[0038] β-GC(nmol / min / ml)=(△A+0.0027)÷0.00543×V reverse total÷V sample÷T=61.39×(△A+0.0027)

[0039] (2) Determination of exo-β-1,4-glucanase (C1) activity

[0040] The content of reducing sugar produced by the degradation of microcrystalline cellulose catalyzed by exo-β-1,4-glucanase was determined by 3,5-dinitrosalicylic acid method.

[0041] C1(ug / min / ml)=1000×(△A+0.0673)÷6.4...

Embodiment 2

[0059] Example 2 Degradation Effect of Single Bacteria and Composite Bacteria on Straw

[0060] Weigh a certain amount of straw powder and place it in a 250ml triangular flask, absorb the same amount of bacterial solutions into the triangular flask, mix them evenly, put them in an incubator at 55 degrees for cultivation, and after 15 days, dry the samples and weigh them to obtain Straw without inoculation was used as a control, and the degradation rate of straw was calculated by weight loss method.

[0061] results and analysis

[0062] Effects of different bacterial flora combinations on straw degradation as follows: figure 1 shown. Among all single-bacteria treatments, No. 5 bacteria (NJAU-N20) had the highest degradation efficiency of 20.75%. In the combination of the two bacteria, the combination of bacteria No. 3 and No. 5 (NJAU-F4-5+NJAU-N20) had the highest degradation rate of 20.57%. Among the 3 bacteria combinations, the combination of bacteria No. 3, No. 5 and No...

Embodiment 3

[0064] Application of embodiment 3 functional bacteria in pure straw composting

[0065] The optimal single bacterium NJAU-N20 and NJAU-N30+NJAU-F4-5+NJAU-N20 compound bacterial agent screened out were used in the in-situ composting test of pure straw as raw material in the fertilizer factory of Ao Ganlin Co., Ltd., Leshan City, Sichuan Province. Three treatments were designed in the experiment: straw, straw+single bacteria, straw+combined bacteria, marked as J1, J2, J3 respectively.

[0066] Test procedure

[0067] 1. Raw material preparation: chop the corn stalks in the outdoor storage yard.

[0068] 2. Ingredients: add high-efficiency single bacteria and combined bacteria to the chopped corn stalks at a ratio of 1%. At this time, the initial water content of the straw group is 70%, and a control group without inoculation is designed.

[0069] 3. Cloth: Use a forklift to transfer the mixture to the fermentation bin for strip fermentation. After the cloth is finished, colle...

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Abstract

The present invention provides an efficient wood fiber degradation composite bacteria agent and an application thereof in composting. For further improving the composting effect of livestock and poultry breeding feces and straw wastes, a composite bacteria agent containing Rhizomucor miehei NJAU-F4-5, Brevibacillus reuszeri NJAU-N20 and bacillus sp. NJAU-N30 is developed, thereby providing composite bacteria resources and technical support for efficient composting of the livestock and poultry breeding feces and the straw wastes. The composite bacteria agent is applied to acceleration of composting fermentation. A fermentation process comprises the following steps of uniformly mixing the livestock and poultry breeding feces and straws according to the certain C / N, inoculating the liquid composite bacteria agent, building a strip-shaped stack, and by adopting a strip-shaped stack type fermentation process, performing turning-throwing fermentation. Compared with the non-addition of bacteria and the addition of single bacteria for treatment in a control, the addition of the composite bacteria agent effectively promotes the temperature rise of a compost body in the initial stage of composting, facilitates the compost body to enter the cooling stage in advance, promotes the increase in germination index of a compost product and effectively drives the compost maturity.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and discloses a microbial composite bacterial agent for efficiently degrading wood fiber and its practical application in production. Background technique [0002] China is a big agricultural country in the world, and it is also a country with a lot of straw resources. After harvesting crops, more than 900 million tons of straw will be produced every year. In recent years, the country has successively promulgated laws and regulations prohibiting the burning of straw; the biogasification technology of straw is currently immature, and it is not easy to promote it in rural areas; open-air stacking cannot efficiently degrade straw due to the lack of functional microorganisms. In addition, the proportion of my country's animal husbandry output value is increasing. On the other hand, most farmers in my country no longer raise pigs and other poultry. my country's animal husbandry has complete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20C05F11/08C05F15/00C12R1/645C12R1/01C12R1/07
CPCC05F3/00C05F11/08C12N1/14C12N1/20C05F11/00
Inventor 沈其荣何宙阳李荣刘东阳沈标徐谞
Owner NANJING AGRICULTURAL UNIVERSITY
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