Strain of saccharomycopsis fibuligera and application thereof

A technology for decapsulating and laminating, yeast, applied in the application, biochemical equipment and methods, organic fertilizers and other directions, can solve the problem of α-amylase activity, protease activity is not disclosed to produce other enzymes and the size of enzyme activity, and the variety of enzymes produced is rich. and high enzyme activity, acid resistance of undisclosed strains, etc., to achieve the effect of improving the quality of fruits and vegetables, high enzyme activity, and vigorous growth.

Active Publication Date: 2018-07-06
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Chinese patent CN200710064203 discloses a fertilizer starter and its preparation method and application. The raw material formula of the fertilizer starter includes: 10-80 parts of fermentation industrial waste residue, 5-30 parts of straw, 5-30 parts of cake, bran and 10-30 parts of rice bran, 1.5-2 parts of urea, 0.5-1 part of NaCl and / or glucose, and Azotobacter chroococcum, Bacillus megaterium, Bacillus licheniformis, Lactobacillus plantarum ( Lactobacillus plantarum) and 1-5 strains in Saccharomycopsis fibuligera) and Saccharomycopsis fibuligera. The function of Saccharomycopsis fibuligera disclosed in this patent is to quickly remove soil compaction, and Utilize the characteristics of wide range of substances, fast growth and easy decomposing, but the patent does not disclose the characteristics of acid resistance, rich enzyme production and high enzyme activity of the bacteria, and the characteristics of rich enzyme production and high activity are important indicators to ensure decomposing
[0022] The S. forticulata yeast described in the above patents is used in the brewing or feed fermentation industry, but its application in the field of distiller's grains is not disclosed; and the yeast S. forticulata used in the above patents has no activity except for α-amylase activity and protease activity. Publicly disclose whether other enzymes are produced and the activity of the enzymes

Method used

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  • Strain of saccharomycopsis fibuligera and application thereof
  • Strain of saccharomycopsis fibuligera and application thereof
  • Strain of saccharomycopsis fibuligera and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] The separation and purification, screening and identification of embodiment 1 yeast Y-1

[0071] 1) Separation and purification: Accurately weigh 10g of distiller's grains sample from the natural accumulation and fermentation of Wuliangye Group in Yibin City, Sichuan Province, add it to a sterilized triangular flask with glass beads containing 100mL of cooled sterile water, and shake it on a shaker at 200r / min Mix evenly for 30 minutes, let it stand for 5 minutes, absorb 1 mL of the supernatant, and serially dilute to obtain 10 -1 -10 -5 Concentration, select 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Use a pipette to absorb 100 μL of each gradient suspension, and spread it evenly on the YPD plate with streptomycin sulfate with a spreader. The final concentration of Streptomyces sulfate in the medium is 30 μg / mL. For bacterial water control, place the plate upside down in the incubator and incubate at 30°C for 7 days. Each treatment is set up to repeat 3 groups horizontall...

Embodiment 2

[0112] Example 2 Low pH Tolerance Test of Saccharomyces fornicata Y-1

[0113] (1) Method: Pick an appropriate amount of yeast Y-1 and insert it into the YPD liquid medium, and culture it with shaking at 180 rpm at 30°C for 48 hours to obtain the seed liquid. pH value (1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5) in the Erlenmeyer flask of sterilized YPD liquid medium, each pH value was repeated three times, and those without inoculation were Blank control, 30°C, 180r / min shaking culture for 48h, observe whether the bacterial liquid in the Erlenmeyer flask is turbid and turbid at each pH, ​​and measure the bacterial activity of the turbid bacterial liquid at the same time;

[0114] (2) Test results: Except for the pH values ​​of 1.0, 1.5, 2.0 and the CK group bacteria liquids were still clear, the YPD liquid culture media with different pH (2.5-6.5) were all turbid, indicating that S. It can grow within the range of pH 2.5-6.5, and the bacteria suspension with diffe...

Embodiment 3

[0115] Embodiment 3 solid-state fermentation of Saccharomyces fornicata Y-1

[0116] (1) Strain activation: use an inoculation loop to pick an appropriate amount of Saccharomyces fumigatus Y-1, streak it onto the PDA plate medium, and culture it at 30°C for 48 hours;

[0117] (2) Preparation of the primary seed liquid: pick an appropriate amount of activated Saccharomyces fornicosa Y-1 with an inoculation loop and inoculate it in the YPD liquid medium, and culture it with shaking at 200 rpm at 30°C for 24 hours to obtain the primary seed liquid;

[0118] (3) Preparation of the secondary seed liquid: inoculate the primary seed liquid into the YPD liquid medium again according to the inoculum amount of 1% (v / v), culture at 30° C. with shaking at 180 rpm for 36 hours, and obtain the secondary seed liquid;

[0119] (4) Solid-state fermentation research:

[0120] ① Mixing material: according to the formula of solid fermentation medium A, first use 500mL tap water to mix glucose, p...

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Abstract

The invention belongs to the technical field of microbial fermentation, specifically belongs to the technical field of microbial decomposing, in particular relates to a strain which produces diversified enzymes with high enzyme activity and good acid resistance, enables distillers' grains to warm quickly and keep high temperature for a long time, and decomposes the distillers' grains completely, and relates to application of the strain. The strain is (Saccharomycopsis fibuligera)Y-1, and the preservation number is CGMCC No. 15255. The activity of an alpha-amylase produced by the strain is 2.56times higher than the currently known highest enzyme activity (7425.4 U / g); at the same time, the strain has the ability of producing glucoamylase, cellulase (CMC enzyme), cellulase (FPA enzyme), neutral protease, acid protease, lipase and other 7 enzymes, can produce diversified enzymes with high enzyme activity, and is applied to a microbial agent for fast decomposing to quickly and thoroughlydecompose the distillers' grains.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to the technical field of microbial decomposing, and in particular relates to a strain of spores of the distiller's grains, which can produce many kinds of enzymes, have high enzyme-producing activity, good acid resistance, fast heating of distiller's grains, long-lasting high temperature, and thorough decomposing of distiller's grains. Yeast, and relates to the application of the strain. Background technique [0002] Distiller's grains are the residual solids after cooking, saccharification and fermentation, distillation and other processes with sorghum, wheat, corn, rice, rye, etc. High acidity, high acidity, if it is allowed to discharge and is not treated in time, it will deteriorate and seriously pollute the environment. [0003] At present, many large-scale liquor companies choose to use distiller's grains for the second time, that is, to build steam workshops,...

Claims

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Application Information

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IPC IPC(8): C12N1/16C05G3/00C05G3/04C05F15/00C05F17/00C12R1/645C05G3/80
CPCC05F5/008C05F17/00C05G3/00C12N1/16C05G3/80C12N1/145C12R2001/645C05F11/02C05F11/08Y02W30/40
Inventor 李玲娣赵敬敬李爽王江王楠范君姣杜英侠袁志新刘桀
Owner 陕西枫丹百丽生物科技有限公司
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