The invention discloses a method for preparing
ganoderma lucidum strains. The method comprises the following preparation steps of (1) preparation of a culture medium, wherein the culture medium comprises, by weight, 200 g of potatoes, 20 g of
agar, 20 g of glucose, 2 g of peptone, 2 g of multivitamins, 2 g of
potassium dihydrogen
phosphate, 0.5 g of
magnesium sulfate and 1000 ml of
distilled water; (2) preparation of the strains, wherein preparation of the strains includes the following preparation steps that a, fresh
ganoderma lucidum is selected for tissue separation; b, seeds are taken; c,
inoculation blocks are taken and inoculated into a
test tube culture medium; d, the
inoculation blocks are placed in a biochemical culture box for culture to obtain mother species; e, wood chips and the like are taken and placed in a
bottle, and the mother species are inoculated into the
bottle and grow for 45 days to obtain original species; f, the wood chips and the like are taken and placed inanother
bottle, and the original species are inoculated into the bottle and grow for 45 days to obtain the strains. The method has the advantages that by adopting the method, the high-quality
Ganoderma lucidum strains which are most suitable for large-scale planting can be obtained, the cultured
Ganoderma lucidum strains are basically at the same strain age, and hyphae have high permeability and grow vigorously.