41results about How to "Consistent bacterial age" patented technology

The purpose of the present invention is to provide a kind of technology of factory-produced air-permeable bacterium bag of shiitake mushroom: adopt air-permeable plastic bag as the bacterium bag container of shiitake cultivation, through the activation cultivation of shiitake mushroom strain, the cultivation of preparing barley cultivar and inoculation of cultivar In the step of cultivating mushroom bags in air-permeable bags, the air-permeable mushroom bags of shiitake mushrooms are cultivated, and the mushrooms are cultivated in a shelf-type factory through color change; the present invention adopts air-permeable plastic bags combined with the special manufacturing steps of the present invention, which can be mass-produced rapidly The shiitake mushroom bag can reduce the production cost at the same time, ensure the production quality of the shiitake mushroom bag, shorten the bag making and cultivation cycle, easy to operate, easy to mechanized production, increase the utilization rate of the cultivation site, save labor, and realize the rapid factory cultivation of the shiitake mushroom. Has significant economic benefits.

The invention discloses a method for liquid fermentation cultivation of Agaricus bisporus strain, which includes: firstly, inoculating activated slant culture 0.5cm2 into a 250mL culture bottle containing 60-120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, a test tube of strain can be multiplied by 200000 times by five levels of cultivation, the secondary liquid strain is evidently faster than traditional solid spawn in growth speed, and the speed is increased by 33%. In addition, the growth speeds of all levels of the liquid strain are nearly equal, the average fullness time is 27.5 days, and the method is absolutely applicable to practice of factory production.

The invention relates to the field of mushroom cultivation processes, in particular to a method for producing solid liquefied strains of oyster mushrooms. First-grade liquid strains are inoculated in culture bottles to obtain liquid strains through cultivation; a solid culture compost is prepared, the liquid strains are cultivated in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, light-shielding cultivation is performed, cultivation humidity ranges from 50% to 60%, and the strain bottles can be full of strains after cultivation for 20 to 30 days; the strain bottles full of strains and free of contaminating microbes are selected out, and the strains are smashed in a smashing machine and are inoculated in sterile water to directly produce strains. By means of the method, strain growing speed is high and can be 1 / 3 higher than the solid strain growing speed, inoculation expanding of three to four times is not needed, the contamination problems in the inoculation expanding process are decreased, and the time required by propagation expanding is saved; growing points are more than growing points of common liquid strains, strains are consistent in growing speed and are relatively consistent in age, fruiting mushrooms are relatively order and good in quality, and fruiting body yield can be improved by 20%-30%; the sterile water is adopted to liquefy hyphae, mould and other contaminating microbes do not grow, and pollution rate can be reduced by 5%-10%.

The invention relates to a method for producing shell strains by using a shell-shaped strain inoculator. According to the technical scheme, a formula of a culture medium of the shell strains is composed of a main ingredient and accessories, wherein on the basis of 10000 shell-shaped strain inoculators, the main ingredient is 30-50kg of sawdust; and the accessories are composed of: 7-8kg of wheat bran, 0.5-1.5kg of corn flour, 0.2-0.6kg of gypsum, 0-0.5kg of lime, 0.1-0.2kg of monopotassium phosphate, 0.05-0.1kg of magnesium sulfate, 0.4-0.5kg of peptone and 0.4-0.5kg of glucose. After the main ingredient and accessories are stirred, filled into bottles, sterilized and subjected to inoculated culture, the shell strains are obtained. Compared with a conventional solid strain, the method provided by the invention has the advantages that the operation is convenient, the strain age is consistent, the strain purity is high and the seed applying cost is reduced; the time for culturing hyphaeis shortened by 10-15 days; and when a culturing bag is produced, the inoculation survival rate is increased to 100%, the pollution rate is reduced by 5% and the yield is increased by 5-10%.

The invention provides a method of producing Agrocybe aegerita strains by liquid culture and a preservation method of the Agrocybe aegerita strains. The method includes the steps and process parameters: liquid culture efficient strains are screened, namely the liquid culture strains with many pellets, uniform size and many surface burrs are optimally selected, the amount of culture pellets is 200-260 / ml, the pellets are 1.0-1.5mm in diameter, a shake-flask culture formula includes 1-3% of glucose, 2-4% of corn flour, 0.2-0.4% of yeast powder, 1.0-3.0% of wheat bran, 0.5-1.5% of KH2PO4 and 0.5-1.0% of MgSO4, with natural PH value, the culture medium formula for fermenting tank culture of Agrocybe aegerita liquid strains is provided, and process conditions for fermenting tank culture of Agrocybe aegerita liquid strains are established. The key technical problems of production of Agrocybe aegerita strains are solved by the production of Agrocybe aegerita strains by liquid culture; the production cycle is short, production cost is low, fruiting is even after inoculation, and management is facilitated.

The invention discloses a method for liquid fermentation cultivation of Pleurotus cornucopiae strain, which includes: firstly, inoculating slant mother culture into a 250mL culture bottle containing 120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for fermentation cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, the liquid strain is evidently faster than traditional solid spawn in growth speed, the culture bottle can be full of mycelia in 16 days, the time is shortened evidently, the growth speed of the liquid strain by inoculation is increased by 75%, and the method is absolutely applicable to practice of factory production.