41results about How to "Consistent bacterial age" patented technology

The invention discloses a method for liquid fermentation cultivation of Agaricus bisporus strain, which includes: firstly, inoculating activated slant culture 0.5cm2 into a 250mL culture bottle containing 60-120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, a test tube of strain can be multiplied by 200000 times by five levels of cultivation, the secondary liquid strain is evidently faster than traditional solid spawn in growth speed, and the speed is increased by 33%. In addition, the growth speeds of all levels of the liquid strain are nearly equal, the average fullness time is 27.5 days, and the method is absolutely applicable to practice of factory production.

The invention relates to the field of mushroom cultivation processes, in particular to a method for producing solid liquefied strains of oyster mushrooms. First-grade liquid strains are inoculated in culture bottles to obtain liquid strains through cultivation; a solid culture compost is prepared, the liquid strains are cultivated in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, light-shielding cultivation is performed, cultivation humidity ranges from 50% to 60%, and the strain bottles can be full of strains after cultivation for 20 to 30 days; the strain bottles full of strains and free of contaminating microbes are selected out, and the strains are smashed in a smashing machine and are inoculated in sterile water to directly produce strains. By means of the method, strain growing speed is high and can be 1 / 3 higher than the solid strain growing speed, inoculation expanding of three to four times is not needed, the contamination problems in the inoculation expanding process are decreased, and the time required by propagation expanding is saved; growing points are more than growing points of common liquid strains, strains are consistent in growing speed and are relatively consistent in age, fruiting mushrooms are relatively order and good in quality, and fruiting body yield can be improved by 20%-30%; the sterile water is adopted to liquefy hyphae, mould and other contaminating microbes do not grow, and pollution rate can be reduced by 5%-10%.

The invention provides a method of producing Agrocybe aegerita strains by liquid culture and a preservation method of the Agrocybe aegerita strains. The method includes the steps and process parameters: liquid culture efficient strains are screened, namely the liquid culture strains with many pellets, uniform size and many surface burrs are optimally selected, the amount of culture pellets is 200-260 / ml, the pellets are 1.0-1.5mm in diameter, a shake-flask culture formula includes 1-3% of glucose, 2-4% of corn flour, 0.2-0.4% of yeast powder, 1.0-3.0% of wheat bran, 0.5-1.5% of KH2PO4 and 0.5-1.0% of MgSO4, with natural PH value, the culture medium formula for fermenting tank culture of Agrocybe aegerita liquid strains is provided, and process conditions for fermenting tank culture of Agrocybe aegerita liquid strains are established. The key technical problems of production of Agrocybe aegerita strains are solved by the production of Agrocybe aegerita strains by liquid culture; the production cycle is short, production cost is low, fruiting is even after inoculation, and management is facilitated.