47results about How to "Inoculation speed" patented technology

The invention relates to an inoculation method of an edible fungus, particularly to a highly efficient inoculation method of the cultivated stems of the edible fungus. The method comprises steps of: construction of a solar greenhouse plastic fungus producing shed; preparation of bagged fungus stems; preparation of medium: 82 percent of saw dust, 17 percent of wheat bran and 1 percent of gypsum; mechanical bagging, sterilization under atmospheric pressure and then moving into the fungus producing shed for cooling; inoculation in the fungus producing shed: after routine sterilization, cooled fungus stems are piled in single layer from east to west, 4 pores are pricked on each stem, then inoculation is carried out and thin film is used for covering. After the first layer of the stems are inoculated, piling in single layer is carried out again from east to west and then inoculation is carried out, and repeat the operation; each pile has 10 to 12 layers; the fungus stems after being inoculated are kept immovable at original places for hypha growing, piles are shifted when the hypha seals the pores of strain or connects with each other, and turn into fungus growth management. The inoculation method of the invention has the advantages that: integrates the inoculation place and the hypha growing and fungus producing place, reduces costs of equipment and manpower, needs no externally sleeved bag, thus saving time, labor and cost; allows cooperation of a plurality of people, thus increasing production efficiency by 1 time; has high inoculation speed, thus reducing the settling probability of mixed bacterium and high rate of finished products. Therefore, the method is applicable to scaled cultivation or factory production.

The invention relates to a device for inoculating wound roots of plants and an application method thereof. The device is connected onto a spray rod of an agricultural sprayer, and is formed by a connecting rod, a cutting head and liquid outlet holes, wherein the connecting rod is of a hollow structure; one end of the connecting rod can be detachably connected with the spray rod of the agricultural sprayer; the other end of the connecting rod is connected with the cutting head; the cutting head is of a hollow structure; the front end of the cutting head is in a wedged shape; a plurality of liquid outlet holes are formed in a wedge-shaped slope; and opening parts of the liquid outlet holes are projected out of the surface of the wedge-shaped slope. According to the device, screws of the connecting rod and screws of the spray rod of the agricultural sprayer are matched, and the use method is simple and convenient. The device is directly fixed on the spray rod without a spray head and is used for inoculating plant endophytes and plant pathogens with infected roots.

The invention discloses a formula of edible mushroom granules and a production process. The formula of the culture medium of the fungus consists of the following components: 97.5% of sorghum grains, 1.5% of light calcium carbonate and 1% of glucose. Its main production process includes: material selection→rinsing→soaking→cooking→removal of free water→stirring→bottling→stopping→wrapping and tying→autoclaving→out of the pot, inoculation→mycelium cultivation→one shake flask → Secondary shake flask → Finished strain. In the production process of the present invention, a small bottle-mouth container bottle is used as the packaging material of the bacterial strain, and the film package of the sealed tampon can not only meet the oxygen demand for the growth of mycelia in the container bottle, but also reduce the contamination of the bacteria by bacteria; The shaking bottle process makes the mycelium filling speed more than double, and the upper and lower bacterial ages are the same when the mycelium is full. The strains produced by this production process have high purity, fast inoculation speed, fast germination, and good food, and are welcomed by the majority of mushroom farmers.

The invention relates to the technical field of vaccines, in particular to a rapid inoculation method for animal vaccines. The method comprises the steps of liquid extracting, liquid mixing, injectingand sterilizing; the inoculation method adopts an inoculating device for inoculation, and the inoculating device comprises a needle-punching mechanism a mixing mechanism, a vaccine extraction mechanism, a vaccine adjuvant extraction mechanism, a protection mechanism and a pushing mechanism; the needle-punching mechanism is fixedly connected to one end of the mixing mechanism; the other end of themixing mechanism is communicated to the vaccine extraction mechanism and the vaccine adjuvant extraction mechanism; the vaccine extraction mechanism and the vaccine adjuvant extraction mechanism arearranged mutually independently; the mixing mechanism is fixedly connected to the protection mechanism; the vaccine extraction mechanism and the vaccine adjuvant extraction mechanism are disposed at the inside of the protection mechanism; the pushing mechanism is installed inside the needle-punching mechanism. The method has the advantages of rapid, safe and reliable inoculation.

The invention discloses a large-scale aseptic-manipulation continuous production equipment and relates to the technical field of structures of large-scale aseptic-manipulation continuous production lines. The production equipment has the structure that a conveyor is arranged on the frame; an inlet platform, an inlet buffering barn, a sterilizing barn, a middle buffering barn, an inoculation barn,an outlet buffering barn and an outlet platform are orderly arranged above the conveyor from left to right; an ozone generator is arranged above the inlet buffering barn and is connected with the sterilizing barn through a pipeline; the inlets and the outlets of the inlet buffering barn, the middle buffering barn and the outlet buffering barn are respectively provided with a movable curtain; a dehumidification window is arranged above the inoculation barn; both sides of the inoculation barn are respectively provided with three pairs of protective sleeves; and an object table is arranged in the middle of the interior of the inoculation barn. The production equipment is an inoculation machine having the functions of environment sterilization, material conveying, oxygen enhancement, dehumidification and continuous inoculation, greatly increases the inoculation speed and the certified product rate, reduces the labor intensity and has the advantages of no sterilization residue, no damage and low cost.

The invention discloses an edible fungus liquid strain storing and inoculating integrated machine. The edible fungus liquid strain storing and inoculating integrated machine is formed by connection of a storing device and an inoculating device. The storing device comprises a liquid strain storage bottle, a bottle cap and an O-shaped seal ring, wherein the O-shaped seal ring is arranged between the bottle neck and the bottle cap of the liquid strain storage bottle. The inoculating device comprises an inoculating syringe needle, a bend and a silicon rubber hose, wherein the inoculating syringe needle is connected with the silicon rubber hose through the bend, a hole is formed in the bottle cap, the bend and the silicon rubber hose penetrate through the hole to be inserted into the liquid strain storage bottle, and the external diameter of the bend is matched with the internal diameter of the hole. The edible fungus liquid strain storing and inoculating integrated machine has the advantages that the structure is simple, storing and inoculating of liquid strains are integrated, operation is simple and convenient, the inoculating speed is high, labor intensity of workers can be relieved obviously, and production efficiency is improved.

The invention discloses a pholiota nameko three-grade seed production method taking amorpha fruticosa braches as base raw materials. The pholiota nameko three-grade seed production method comprises the following steps: obtaining branch wood sections, treating the branch wood sections, distributing wheat bran on the branch wood sections, preparing an auxiliary culture base material, forming a strain material bag, sterilizing at high pressure and inoculating. The auxiliary culture base material is prepared from the following materials in percentage by mass: 72%-85% of saw dust, 15%-25% of wheat bran, 0.5%-2% of gypsum and 0.5%-2% of lime; water is added into the materials and is uniformly mixed, and the moisture content is controlled to be about 65%. By the aid of the method for preparing pholiota nameko three-grade seeds, the fungi growth time can be shortened for 20-30 days, the inoculating speed is rapid and the pollution rate is greatly reduced; mycelia are thick and dense and grow vigorous; strains on the upper part can be prevented from being aged and the strain preservation period is prolonged by more than 30-40 days.