The invention discloses a method for producing oyster mushroom strains from birch chopsticks. The method comprises four steps of: performing pretreatment on the chopsticks, preparing a culture medium for the chopsticks, performing inoculation culture and transferring the strains. According to the method disclosed by the invention, by carrying out pretreatment on the birch chopsticks, on the one hand, harmful substances in the birch chopsticks can be fully decomposed, and on the other hand, convenience is brought to the insertion of the birch chopsticks into a mycelium mass during the inoculation. When a culture medium for the birch chopsticks is prepared, as birch contains a large number of nutrient components required for growth of oyster mushrooms, only sucrose, a compound fertilizer, wheat bran and corn flour need to be further added, therefore the components in a nutrient solution are greatly simplified and the preparation process is also trouble-saving and effort-saving. When the inoculation is performed, only the chopsticks need to be directly inserted into the mycelium mass for completion, the operation process is convenient, fast, effort-saving and labor-saving and the pollution rate is very low. Mycelia are fully grown all over the whole chopstick strains after culture, the cell ages after the inoculation are short and consistent, the strains are fast in growth speed, the mycelia are robust, and the strains are high in purity and strong in resistance to other fungi. The method disclosed by the invention further has the more prominent advantages of low production cost and very significant economic benefits.