47results about How to "Inoculation speed" patented technology

The invention relates to an inoculation method of an edible fungus, particularly to a highly efficient inoculation method of the cultivated stems of the edible fungus. The method comprises steps of: construction of a solar greenhouse plastic fungus producing shed; preparation of bagged fungus stems; preparation of medium: 82 percent of saw dust, 17 percent of wheat bran and 1 percent of gypsum; mechanical bagging, sterilization under atmospheric pressure and then moving into the fungus producing shed for cooling; inoculation in the fungus producing shed: after routine sterilization, cooled fungus stems are piled in single layer from east to west, 4 pores are pricked on each stem, then inoculation is carried out and thin film is used for covering. After the first layer of the stems are inoculated, piling in single layer is carried out again from east to west and then inoculation is carried out, and repeat the operation; each pile has 10 to 12 layers; the fungus stems after being inoculated are kept immovable at original places for hypha growing, piles are shifted when the hypha seals the pores of strain or connects with each other, and turn into fungus growth management. The inoculation method of the invention has the advantages that: integrates the inoculation place and the hypha growing and fungus producing place, reduces costs of equipment and manpower, needs no externally sleeved bag, thus saving time, labor and cost; allows cooperation of a plurality of people, thus increasing production efficiency by 1 time; has high inoculation speed, thus reducing the settling probability of mixed bacterium and high rate of finished products. Therefore, the method is applicable to scaled cultivation or factory production.

The invention relates to the technical field of vaccines, in particular to a rapid inoculation method for animal vaccines. The method comprises the steps of liquid extracting, liquid mixing, injectingand sterilizing; the inoculation method adopts an inoculating device for inoculation, and the inoculating device comprises a needle-punching mechanism a mixing mechanism, a vaccine extraction mechanism, a vaccine adjuvant extraction mechanism, a protection mechanism and a pushing mechanism; the needle-punching mechanism is fixedly connected to one end of the mixing mechanism; the other end of themixing mechanism is communicated to the vaccine extraction mechanism and the vaccine adjuvant extraction mechanism; the vaccine extraction mechanism and the vaccine adjuvant extraction mechanism arearranged mutually independently; the mixing mechanism is fixedly connected to the protection mechanism; the vaccine extraction mechanism and the vaccine adjuvant extraction mechanism are disposed at the inside of the protection mechanism; the pushing mechanism is installed inside the needle-punching mechanism. The method has the advantages of rapid, safe and reliable inoculation.

The invention discloses a pholiota nameko three-grade seed production method taking amorpha fruticosa braches as base raw materials. The pholiota nameko three-grade seed production method comprises the following steps: obtaining branch wood sections, treating the branch wood sections, distributing wheat bran on the branch wood sections, preparing an auxiliary culture base material, forming a strain material bag, sterilizing at high pressure and inoculating. The auxiliary culture base material is prepared from the following materials in percentage by mass: 72%-85% of saw dust, 15%-25% of wheat bran, 0.5%-2% of gypsum and 0.5%-2% of lime; water is added into the materials and is uniformly mixed, and the moisture content is controlled to be about 65%. By the aid of the method for preparing pholiota nameko three-grade seeds, the fungi growth time can be shortened for 20-30 days, the inoculating speed is rapid and the pollution rate is greatly reduced; mycelia are thick and dense and grow vigorous; strains on the upper part can be prevented from being aged and the strain preservation period is prolonged by more than 30-40 days.