Fast manufacture method for liquid strain

Abstract

The invention belongs to the technical field of biology deep fermentation technology and particularly relates to a fast manufacture method for liquid strain. The method comprises the steps that for a culture medium, rice powder serves as a carbon source, sugar serves as an auxiliary carbon source, corn powder serves as a nitrogen source, peptone and yeast extract (powder) serve as auxiliary nitrogen sources, monopotassium phosphate and magnesium sulfate serve as inorganic salts, and water serves as a carrier; a glass container serves as a culture container; inoculation is performed after a liquid medium is sterilized, and the liquid strain is obtained through a rotary type shaking table. The fast manufacture method for the liquid strain has the advantages that cheap plant starch is used as the main carbon source and the nitrogen sources, and nutrition-allocated proportion is reasonable and comprehensive; the prepared liquid medium is appropriate in viscosity; a culture period is short, and the liquid strain with good quality, strong vitality and different sizes can be cultured in 2-3 days; at the time of inoculation, the fast manufacture method for the liquid strain is simple, convenient and fast; when a solid culture material is inoculated, the material flows fast and is easy to scatter, many strain running points appear and germinate fast and the like.

Description

technical field [0001] The invention belongs to the technical field of biological submerged fermentation, and in particular relates to a method for rapidly producing liquid strains. technical background [0002] In the production process of edible fungi, the key to success and failure is the strain; the most worrying investment in production is also the strain. The purity, vigor, cultivation time and resistance to impurities of the strain determine the success or failure of the production. In order to achieve high-quality, industrialized, and standardized production, and at the same time improve cultivation efficiency, it is necessary to solve the current backward mode of strain production. [0003] Traditional solid strains require multi-level transfer, and one transfer takes half a year. During production, we are afraid of pollution before and aging later, which is cumbersome and complicated. Due to the slow germination of solid bacteria, the pollution remains high, and...

AI Technical Summary

Problems solved by technology

[0005] In the traditional production of liquid strains, due to the unreasonable nutrient ratio during the preparation of the liquid medium, the viscosity of the liquid strains is not suitable, resulting in a production period of 12-15 days or longer to complete the entire fermentation process Time, the mycelium (ball) size is inconsistent, and the quality of the strain is not good