The invention provides a nursery stock nutrition medium preparation method which comprises the following steps: a, mixing, dewatering and drying clay, cow dung and plant waste leaves and then grindingto obtain a first raw material; b, adding corn stalk, plant cellulose, magnesium sulfate and a nutrient solution into mushroom dregs, fully mixing, then sealing, heating and fermenting, then adding zeolite powder and saltpeter powder and evenly stirring to obtain a second raw material; c, adding ailanthus leaves, herba houttuyniae, a soil nutritional agent and a deinsectization agent into distilled water, soaking, utilizing a pulp grinding machine to grinding pulp and filtering, adding the first raw material, the second raw material and expanded perlite into filtrate, adding an obtained mixture into a ball mill to be ball milled, then filtering, adding ammonium phosphate and gibberellin into obtained filtrate and evenly stirring to obtain a third raw material; d, drying the third raw material and sealing and packaging to obtain the nursery stock nutrition medium. The preparation method disclosed by the invention is simple; the raw materials are natural, are environment-friendly and are easy to obtain; the prepared nutrition medium has the advantages of stable performance and good fertilizer efficiency.