Inoculation method of tissue culture seedlings of potatoes
A technology for tissue culture seedlings and potatoes, applied in the field of plant cultivation, which can solve the problems of complex operation, susceptibility to virus, time-consuming and laborious, etc., and achieve the effect of simple operation, low probability of virus contamination, and fast breeding speed
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Embodiment 1
[0022] Select and cultivate 10 strains of strong tissue-cultured virus-free seedlings with a seedling age of 15 days, and then adopt the technical scheme of the present invention to inoculate.
[0023] 1. Disinfection and sterilization
[0024] (1) Disinfection and sterilization of the medium: the medium used for the tissue culture of the virus-free seedlings of the present invention is a liquid medium, and the prepared nutrient base is sterilized under high temperature and high pressure for 20 minutes, wherein the temperature is 121 ° C, the air pressure 1.11-1.14kpa;
[0025] (2) Disinfection and sterilization of inoculation equipment: irradiate the inoculation room, cultivation room, preparation room, etc. with ultraviolet light, mop and wash the floor with 0.1% benzalkonium bromide, and fumigate with formazan and potassium permanganate from time to time; Soak the scissors, tweezers, and petri dishes with 75% alcohol before inoculation; the scissors and tweezers after soak...
Embodiment 2
[0032] Select and cultivate 10 strains of strong tissue-cultured virus-free seedlings with a seedling age of 15 days, and then adopt the technical scheme of the present invention to inoculate.
[0033] 1. Disinfection and sterilization
[0034] (1) Disinfection and sterilization of the medium: the medium used for the tissue culture of the virus-free seedlings of the present invention is a liquid medium, and the prepared nutrient base is sterilized under high temperature and high pressure for 20 minutes, wherein the temperature is 121 ° C, the air pressure 1.11-1.14kpa;
[0035] (2) Disinfection and sterilization of inoculation equipment: irradiate the inoculation room, cultivation room, preparation room, etc. with ultraviolet light, mop and wash the floor with 0.1% benzalkonium bromide, and fumigate with formazan and potassium permanganate from time to time; Soak the scissors, tweezers, and petri dishes with 75% alcohol before inoculation; the scissors and tweezers after soak...
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