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Method for culturing mesenchyme stem cell from oral cavity tissue

A mesenchymal stem cell and tissue technology, applied in the field of culturing mesenchymal stem cells, can solve the problems of time-consuming and easily polluted dental pulp tissue, and achieve the effects of simple source collection, easy culture and preservation, and material saving.

Inactive Publication Date: 2008-03-19
黄显成
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  • Description
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  • Application Information

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Problems solved by technology

However, these studies are limited to the third molar and deciduous incisors, and the above-mentioned research method needs to cut the tooth from the junction of cementum and enamel to the whole tooth, which is very time-consuming, and the pulp tissue is easy to be contaminated

Method used

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  • Method for culturing mesenchyme stem cell from oral cavity tissue
  • Method for culturing mesenchyme stem cell from oral cavity tissue
  • Method for culturing mesenchyme stem cell from oral cavity tissue

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no. 1 example

[0055] The first embodiment of the present invention is a method for culturing mesenchymal stem cells from oral cavity tissue, please refer to Figure 1, including:

[0056] A. Prepare the pulp sample: soak the tooth sample in buffer solution. Penicillin and streptomycin, or store the tooth sample in a refrigerator at 4°C for a storage time of less than or equal to 48 hours; then grind the handpiece at a high / slow speed to pre-grind a full circle of groove marks on the tooth surface, The grooves have a depth of 0.5-1.0 mm, and the teeth are peeled off with external force to expose the pulp tissue and then extracted. The way of exposing the pulp tissue is a dental tool. In this embodiment, a chisel is used to cut, thump, press or grind the pulp at the groove position to expose the pulp, and then use a sterile surgical tool , such as root canal files, curettes, tweezers and probes, this embodiment uses root canal files to extract dental pulp samples, and the surgical process of ...

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Abstract

The present invention discloses a method for cultivating the mesenchymal stem cells from the oral tissue. A dental pulp or gingival mucosa sample is prepared, dental pulp mesenchymal stem cells are abstracted from the dental pulp tissue of the tooth or gingival mucosa mesenchymal stem cells are abstracted from a gingival mucosa tissue, then, the cells are cultivated, the cells present the kenel of the dental pulp mesenchymal stem cells or kenel of the gingival mucosa mesenchymal stem cells, and the next cultivation and the preservation are operated. The dental pulp mesenchymal stem cells and the gingival mucosa mesenchymal stem cells abstracted through the method of the present invention have the abilities of clustering, differentiating into the substance of bone, differentiating into fat, and differentiating into nerve. The method of the present invention reduces the pollution rate, and leads the mesenchymal stem cells to be easily cultivated and preserved.

Description

technical field [0001] The invention provides a method for cultivating mesenchymal stem cells from oral cavity tissue, which comprises isolating dental pulp mesenchymal stem cells from dental pulp tissue or isolating mesenchymal stem cells from gingival mucosal tissue and culturing them. Background technique [0002] Mesenchymal stem cells and embryonic stem cells are the two main types of stem cells, which are often used in cell or disease treatment research. Mesenchymal stem cells can be isolated from a variety of different tissues, including bone marrow, liver, blood vessels and nerve tissues. Among them, the tissue that is often studied is the mesenchymal stem cells from the bone marrow. This kind of mesenchymal stem cells from the bone marrow has the ability of self-renewal and regeneration, and can also differentiate into a variety of different cell sources. In recent years, literature has pointed out that mesenchymal stem cells from the bone marrow Mesenchymal stem ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N5/0775
Inventor 黄显成
Owner 黄显成
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