32 results about "Cementum" patented technology

The invention discloses a pulp capping agent with bioactivity and a preparation method thereof. The pulp capping agent with bioactivity comprises dentin extraction solution and dentin powder. The pulp capping agent preparation method comprises the following steps: (1) selecting health dental tissue, cleaning by PBS (Phosphate Buffer Solution) to remove periodontal ligament, cementum and dental pulp, and sterilizing; (2) freezing the dental tissue, and grinding the frozen dental tissue to particles; (3) culturing the dental tissue particles in a medium; and (4) filtering the supernatant from the step (3) to obtain the dentin extraction solution, centrifugally cleaning the cultured dentin particles by the PBS, oven-drying, and grinding to obtain the dentin powders. The pulp capping agent can effectively promote the repair of dentin only by blending the dentin extraction solution with the dentin powder, and applying the mixture at a position close to the dental pulp or at a position at which the dental pulp is exposed, thereby improving the success rate of live-marrow conservation, and providing a new treatment means for patients suffering from accident pulp perforation.

The purpose of this study was to identify the periodontal regeneration factors in enamel protein shown to induce cementum- and osteo-promotive activities in vivo. The cementum regeneration (CR), a part of periodontal regeneration, was examined by using experimental cavities prepared in a buccal dehiscence dog model. The CR activity was found in the aggregate consisted of sheath proteins along with a small amount of amelogenins separated from newly formed secretory enamel. The sheath proteins were purified to be homogeneous, and examined for the alkaline phosphatase (ALP) inducing activity of human periodontal ligament (HPDL) cells. Application of 17 kDa sheath protein induced ALP activity in HPDL cells. Peptides synthesized from the sheath protein sequence also showed ALP inducing activity. The 17 kDa sheath protein has cell differentiation activity.

The invention relates to a lay down method for model used in detecting cell differentiation function and the method includes the following steps: (1) preparing heelpiece, enamel matrix protein (EMPs) and myeloid stroma cell; (2) covering the heelsurface of the said heelpiece with EMPs; (3) inoculating the myeloid stroma cell on the heelpiece surface obtained by step (2); (4) placing the heelpiece obtained by step (3) into the politef film pocket; (5) placing the heelpiece covered by the pocket under naked mouse skin. The advantages of this invention include connecting the cell culture in vitro and growth in vivo, insulating the impact of naked mouse cell with screen film and inspecting the impact of EMPs to the marrow stroma cell differentiation, which consequently can provide effective research model for studying EMPs application mechanism. At the 8th week of the experiment, different histological structures are formed by different processing groups and the cellular cementum samples are formed on the heelsurface trained by EMPs, which indicates that the EMPs can improve the marrow stroma cell differentiation to the cementoblastoma and also indicates that the research model is successful.

The invention relates to a manufacturing method of an immediate dental implant with periodontal bioactivity, comprising the following steps: (1) preparing a pure titanium or titanium alloy implant core; (2) preparing a self-curing calcium magnesium phosphate powder; (3) preparing a collagen solution (4) Preparation of artificially synthesized substance P polypeptide; (5) Preparation of SP cross-linked collagen matrix; (6) Calcium magnesium phosphate powder mixed with SP cross-linked collagen matrix to prepare bioactive paste; (7) Bioactive paste Coating the root of the implant core, pressurizing, solidifying and freeze-drying to make an artificial tooth root with periodontal bioactivity; (8) connecting the crown of the implant core to an artificial crown suitable for the shape of the missing tooth to make an immediate implant. By constructing the artificial tooth root with bionic cementum structure, the prepared dental implant has some life characteristics of natural implanted teeth, has the biological activity of autonomous periodontal healing, can be implanted immediately after tooth extraction, significantly shortens the restoration time of missing teeth, and improves planting success rate.

The purpose of this study was to identify the periodontal regeneration factors in enamel protein shown to induce cementum- and osteo-promotive activities in vivo. The cementum regeneration (CR), a part of periodontal regeneration, was examined by using experimental cavities prepared in a buccal dehiscence dog model. The CR activity was found in the aggregate consisted of sheath proteins along with a small amount of amelogenins separated from newly formed secretory enamel. The sheath proteins were purified to be homogeneous, and examined for the alkaline phosphatase (ALP) inducing activity of human periodontal ligament (HPDL) cells. Application of 17 kDa sheath protein induced ALP activity in HPDL cells. Peptides synthesized from the sheath protein sequence also showed ALP inducing activity. The 17 kDa sheath protein has cell differentiation activity.

The invention discloses an application of a fibrous strip for preparing a pulp-perforation cracked molar repairing body. A crack of a pulp-perforation cracked molar is located at a dental crown occlusion face along a developmental groove in a mesial and distal direction and is parallel to a tooth long axis. The depth of the crack is 1.0 mm from the pulp perforation position to the cemento-enamel junction in a root direction, and the width of the crack is 0.01-0.10 mm, and the thickness of the fibrous strip is 1.0-1.5 mm, and the width is 2.0-3.0 mm. The fibrous strip is located at a dental crown occlusion face in a direction vertical to the crack, or the fibrous strip is located in a medullary space to form rectangular laying, or the fibrous strip is located in a medullary space to form annular laying. The thickness of the fibrous strip is 1.0-1.5 mm, and the width is 2.0-3.0 mm. The fibrous strip is added in a resin repairing body, can improve stress concentration on the tip of the pulp-perforation tooth crack from a maxillary first premolar in a mesial and distal direction, and can efficiently prevent further development of a full crown lower crack. An excellent effect is obtained by performing annular laying of the fibrous strip in a direction vertical to the crack level in a medullary space.

The present invention is directed to methods of decalcification and tissue sample preparation that allows for the reproducible quantitative analysis of gene expression in hard tissue samples like bone, mineralizing cartilage and tendon, dentin, cementum and / or enamel that are too hard to section effectively using conventional means.

The invention discloses a pulp capping agent with bioactivity and a preparation method thereof. The pulp capping agent with bioactivity comprises dentin extraction solution and dentin powder. The pulp capping agent preparation method comprises the following steps: (1) selecting health dental tissue, cleaning by PBS (Phosphate Buffer Solution) to remove periodontal ligament, cementum and dental pulp, and sterilizing; (2) freezing the dental tissue, and grinding the frozen dental tissue to particles; (3) culturing the dental tissue particles in a medium; and (4) filtering the supernatant from the step (3) to obtain the dentin extraction solution, centrifugally cleaning the cultured dentin particles by the PBS, oven-drying, and grinding to obtain the dentin powders. The pulp capping agent can effectively promote the repair of dentin only by blending the dentin extraction solution with the dentin powder, and applying the mixture at a position close to the dental pulp or at a position at which the dental pulp is exposed, thereby improving the success rate of live-marrow conservation, and providing a new treatment means for patients suffering from accident pulp perforation.

The invention discloses a high definition print processing method for the surface of a cementum plate and the cementum plate with prints. The processing method comprises the following steps: sanding the cementum plate: a layer of permeable primer is painted on the front of the cementum plate; processing putty; painting a layer of seal undercoat; sanding processing; painting a layer of white primer; sanding processing; painting a layer of super-white paint. The surface of the cementum plate is subjected to special pretreatment, thus colored paint and ink can be stuck on the surface of the cementum plate, and the surface of the cementum plate is provided with a variety of colors, figures or patterns, and the patterns are not easy to fall off. Therefore the cementum plate with the prints is wear-resisting, ageing resistant, not susceptible to pollution and waterproof, and has good fire prevention performance. The cementum plate with the prints is suitable for decorating the indoor wall face. The cementum plate is provided with the fire-prevention, tough, impact resistant and environment-friendly performances of the cementum plate as well as the decoration effects of high imitation wood grain, marbling, personalized patterns, and the preparation cost is lower.

The invention relates to adhesives, particularly to a preparation method of an environment-friendly adhesive. The environment-friendly adhesive is composed of the following raw materials including, by weight parts, 70-95 parts of phthalic anhydride, furfuryl alcohol extracted from natural plant, triethylene glycol, pentanediol, butantriol, zinc oxide, magnesium oxide, chloroprene rubber and elastic rubber, 2-3 parts of softening oil, 20-30 parts of dimethyl carbonate, benzoyl peroxide, ethyl acetate, emulsifier and flour, 5*10-5-9*10-5 parts of nano silver, and fire retardant, wherein the elastic rubber is composed of first elastic rubber and second elastic fiber which are mixed at a weight ratio of 1-3:1. The preparation method of the second elastic rubber comprises heating polyvinyl alcohol solution and waterborne polyurethane water solution, cooling the mixture to 30-50 DEG C and then adding acrylate into the mixture; then adding potassium hydroxide into the mixture and heating the mixture; then adding cementum protein, abietin and plant phenols extracted from natural plant for reaction; then adding sodium benzoate, di-tert-butyl peroxide and the ethyl acetate and mixing the reactants to obtain the environment-friendly adhesive. The environment-friendly adhesive is good in bactericidal activity and high in stability, dispersibility and adhesiveness.