74results about How to "Promote growth and differentiation" patented technology

The present invention relates to an active peptide feed addictive, as a mixture of one or more plant protein and animal protein, which is the product of the following steps: fermenting and degrading the mixture through bacillus and the like, controlling the degree of hydrolysis at 20-35%, obtaining a fermentation supernatant after a separation post-processing process, concentrating drying the supernatant or directly adsorbing the supernatant onto the carrier, and then low temperature drying it. Said active peptide feed addictive is capable of obviously depressing development of bacillus coli and salmonella, and can prevention and cure diarrhea caused by bacilli or lienteric diarrhea; can exert its functions at a very low concentration, with a very intense effect; can regulate stomach and intestine bacterium group and promote animals digestion functions; can excite animal immune function, reinforce animal disease resistance against a plurality of epidemic diseases and greatly reduce the morbidity and death rate; can promote animal development and improve the growth velocity; can improve the feed use ratio and reduce production costs. Applications of the microorganism fermentation degradation method provided by the present invention have a low fabricating cost, and the obtained peptides have many kinds and high content, without environment pollutions.

The invention relates to a preparation method of an oral biofilm. The preparation method comprises the following steps: adding type I collagen protein into an acetic acid solution to prepare a collagen-acetic acid swelling solution; adding chondroitin sulfate and stirring to prepare a collagen-chondroitin sulfate serous fluid; carrying out vacuum freeze-drying; pressing to prepare a collagen composite film; spraying the collagen-chondroitin sulfate serous fluid between two layers of the collagen composite film, and carrying out vacuum freeze-drying; carrying out high temperature vacuum crosslinking; and sterilizing after crosslinking to obtain a collagen-chondroitin sulfate composite oral biofilm. The invention has the following beneficial effects: the oral biofilm prepared by the above method has good histocompatibility, suitable controllable degradability and absorbability, low antigenicity and good plasticity and mechanical property; the oral biofilm is used in oral operation; and the three-dimensional porous structure of the oral film provides a tissue engineering scaffold for repairing of wounded tissues, is beneficial to invasion of cells and differential growth and can induce reparative regeneration of defective tissues, and specific thickness of the biofilm can effectively prevent ingrowth of soft tissue.

The invention provides a porous dental implant capable of carrying a drug sustained release system. The porous dental implant comprises an implant body. An external thread connected with alveolar bone is arranged on the outer surface of the implant body. Porous structures are formed among adjacent threads of the external thread. Pores used for storing sustained release drugs are formed in the porous structures. The invention provides a preparing method of the porous dental implant capable of carrying the drug sustained release system. According to the porous dental implant capable of carrying the drug sustained release system and the preparing method thereof, bonding strength is effectively improved, bonding speed is increased, and a porous structure can be achieved easily.

The invention relates to a plant sprouting and branching accelerator and a preparation method thereof. The plant sprouting and branching accelerator is prepared from the following raw materials in percentage by weight: 6 percent of 6-benzylaminopurine, 2 percent of forchlorfenuron, 5 percent of gibberellins, 2 percent of naphthylacetic acid, 2 percent of compound sodium nitrophenolate, 4 percent of brassinolide, 1 percent of indoleacetic acid, 1 percent of Kang polyphenol, 5 percent of seaweed essence, 5 percent of organosilicon penetrant, 0.1 percent of decavitamin, 30 percent of ethanol, 5 percent of glycerinum and the balance of liquid wool grease. An emulsified liquid obtained by emulsifying the components in an emulsifying machine is a branching agent; the concentration of the ethanol is 65 weight percent; the plant sprouting and branching accelerator has the advantages of strong penetrability, clear targeting effect, remarkable pesticide effect, safety, stability and long lasting period, is more beneficial to the sprouting and branching of a plant, can accelerate the sprouting and branching of current-year, annual and two-year buds of the plant, and can accelerate the sprouting and branching of the plant before sprouting to the overall growing season.

The present invention relates to a method for providing an embedded mammalian cell, comprising the steps of providing an alginate sulfate in aqueous solution; reacting the alginate sulfate to form a hydrogel in a gelation step, providing a precursor cell, and embedding the precursor cell in the sulfated alginate hydrogel in an embedding step, thus yielding an sulfated alginate hydrogel embedded cell. The invention further relates to sulfated alginate hydrogels, and cellular grafts comprising a mammalian cell embedded in sulfated alginate hydrogel.

The invention relates to the technical field of planting, in particular to an efficient planting method for mulberry. The method comprises the following steps of seedling growing, transplanting, pest control and field management. By adopting the planting method, planted mulberry is high in disease resistance and insect resistance, high in yield and strong in pesticide effect, compared with the acre yield of mulberry planted through a traditional method, the yield of folium mori can be increased by 28% or above, the yield of mulberry fruits can be increased by 13.2% or above, and due to the planting method, the acre yield of mulberry trees is effectively improved; the survival rate of seedlings is increased by 9.4% compared with a traditional method; on the basis of the scientific planting method, effective components of mulberry trees can be effectively improved, the pesticide effect is improved, and the requirement of the current market for a large number of mulberry trees is met.

The invention discloses a facility cultivation technique for crop rotation of stropharia rugosoannulata and rice, and belongs to the technical field of edible mushroom cultivation. The facility cultivation technique includes the steps of fermentation of a culture material, construction of a mushroom shed, construction of a ridged bed, seeding, mycelium management and protection, mushroom cultivation and harvesting, and rice planting and harvesting. After the components of the culture material are subjected to composting and fermentation separately, layered laying is adopted, so that nutrient and oxygen supply in the development process of mycelia and fruiting bodies is ensured, the whole fruiting is uniform, the fruiting rate is stable, the total yield is high, the quality is good, the planting cycle is short, and the continuous cropping obstacles can be solved by crop rotation of the stropharia rugosoannulata and rice.

The invention discloses a preparation method of feed with comprehensive nutrition special for loaches. The method comprises the following steps that fish meal, rice flour, broom corn millet, sweet potato powder, potato powder, palm meal, fermented oil tea cake, molasses powder, yeast protein meal and olive meal are mixed and crushed, and peanut shell powder, berberine, cerous citrate and salt areadded for even mixing to obtain a premix; water is added to the premix, steaming and cooling are carried out, allicin, mountain flour, calcium hydrophosphate, isoleucine, lysine and micro-elements areadded for even mixing, granulation and coating are conducted, in the granulation and coating process, an antibacterial coating agent is sprayed, and then the feed with the comprehensive nutrition special for the loaches is obtained. The obtained feed can not only promote fast development of the loaches, make the loaches easily suitable for the breeding environment and improve the survival rate, but also promote differentiation growth of the bone cells and muscle cells of the loaches and improve the immunity of the loaches; meanwhile, the dosage is little, and the nutrition value of the loaches is on a par with that of wide loaches.

The invention provides application of leaf mustard polypeptide to preparation of skin whitening and repair cosmetics. The leaf mustard polypeptide has small molecular weight, can be easily absorbed by the skin and can aim at the problem of pigmentation in the skin damage repair process when being used for preparing the cosmetics; the acceleration of the skin damage repair process is promoted; the skin cell growth differentiation is accelerated; the repair time is shortened; the skin is protected, so that the hyperpigmentation of the skin caused by protection reactivity is relieved.

The invention discloses an external composition for preventing alopecia and promoting hair growth and a preparation method thereof. The method comprises the following raw materials including adenosine, glycyrrhetinic acid, apple polyphenol, baicalin, alcohol and water. The method has the advantages that since hair papilla cells positioned at the central bottoms of hair follicles generate and release proliferation factors, the proliferation and differentiation of hair follicle epithelial stem cells are influenced, and therefore the hair cycle is controlled; the baicalin can maintain the derivatization ability of the hair follicles, the adenosine can improve the proliferation of the hair papilla cells, the glycyrrhetinic acid can prevent the apoptosis of the hair papilla cells, and the applepolyphenol, especially dimers thereof, can promote the differentiation and growth of keratinocytes and protein synthesis. All raw materials of the external composition for preventing alopecia and promoting hair growth are organically combined together, correspondingly the number of the hair follicles can be effectively increased, the vitality of the hair papilla cells in the hair follicles can bemaintained, and the effects of integrally promoting the hair growth and reducing the hair loss are thus achieved.

The invention relates to the technical field of seedling culturing, in particular to a seedling culturing method of yellow peaches. The method comprises steps as follows: seed pretreatment, germination acceleration, sowing and field management. The method adopts a sowing and seedling culturing method, epidermal cells of seeds are effectively promoted to grow and differentiate, the seedling culturing cycle is shortened, the stress resistance and the adaptability of yellow peach seedlings to the environment are improved, and the survival rate is increased. With the adoption of the culturing method, the survival rate of the yellow peach seedlings reaches 90% or higher and is 4.6%-5.9% higher than that of seedlings cultured with a conventional culturing method.

The invention relates to a special feed additive for meat-purpose quail for resisting cold stress and improving meat quality, and the special feed additive is used for the general problems of frequent enteritis and poor meat quality of quail in northeast China caused by cold stress. Various ingredients carry out synergistic interaction to play the functions of resisting cold stress by procyanidolic ologomer and improving the meat quality, which are attempts and breakthroughs in the feed field. The additive is low in use amount, low in cost, environment-friendly, non-toxic and suitable for large-scale popularization and application.

A zinc finger protein gene OsLSD1 of paddy rice contains 3 zinc finger structure domains and can code a protein containing 143 amino acids. Its expression is fully suppressed by rice blast bacteria. Said OsLSD1 protein is a negative regulatory factor of programmed cell death and can promote the growth differentiation of transgenic celli of rice. The transgenic tobacco expressing OsLSD1 has high tolerance to cancerogenic microbial toxin fumonisin B1 (FB1).

The invention relates to the technical field of seedling, in particular to a litsea cubeba seedling method. The method includes the steps of 1), treatment of planting land; 2), pretreatment of seeds;3), sprouting treatment; 4), seedling: 5), water and fertilizer management. With the method, growth differentiation of seed epidermal cells is effectively promoted, seedling period is shortened, stress resistance of young seedlings of the litsea cubeba and adaptability to the environment are improved, and survival rate is increased; the survival rate of the young seedlings of the litsea cubeba averagely reaches more than 93%, and morbidity of the young seedlings of the litsea cubeba is effectively reduced to less than 3.5%.

The invention relates to the technical field of seedling breeding, and particularly relates to a seedling breeding method of passiflora edulis. The method comprises the following steps: (1) base bed treatment, (2) seed pretreatment, (3) germination accelerating treatment, (4) sowing process, and (5) water and fertilizer management. According to the invention, a sowing and seedling breeding methodis adopted, so that the growth and differentiation of seed coat cells can be effectively promoted, the seedling breeding period is shortened, the stress resistance and the adaptability of the passiflora edulis to the environment are improved, and the survival rate is improved. With the adoption of the cultivation method provided by the invention, the survival rate of the passiflora edulis seedlings reaches 96% or above, and the incidence rate of the passiflora edulis seedlings is effectively decreased to 1.6% or below.

The invention aims to provide an anti-aging injection. The anti-aging injection comprises elastin peptide, and is characterized by further comprising human adipose-derived mesenchymal stem cell culture medium supernatant and seaweed extract. Adipose-derived stem cells and the elastin peptide are combined and are assisted by seaweed extract liquor, synthesis of elastin can be promoted, activity ofelastase is inhibited, proliferation and differentiation of epidermal cells are promoted by the aid of vitamin C, oxygen free radicals are removed, skin repair of injured portions is promoted, growthand differentiation of skin cells are promoted by vitamin A, a hematopoietic function of surface skin is enhanced, the structure of skin elastic tissues is reconstructed essentially, and aging of human skin is delayed. The adipose-derived stem cells and the elastin are combined and are assisted by the seaweed extract liquor for facial lifting, the problems of facial sagging, non-elasticity of skinand the like caused by aging of the skin can be solved fundamentally, and a good wrinkle removing effect is achieved.

The invention relates to a three-dimensional co-culture induction method of mesenchymal stem cells, wherein the induction method comprises the following steps: respectively acquiring mesenchymal stem cells and a target cell source; covering the mesenchymal stem cells and a magnetic oxide in gel beads A; covering the target cell source in gel beads B; and co-culturing the mesenchymal stem cells in the gel beads A and the target cell source in the gel beads B by virtue of an inducer so that the mesenchymal stem cells are converted into target cells, and then isolating the gel beads A and the gel beads B in a magnetic field, wherein the magnetic oxide particles are 1-100nm in diameter. The induction method disclosed by the invention not only can achieve a purpose of simplifying the isolation of co-cultured cells, but also can expand a growth space of the mesenchymal stem cells as well as substance signal exchange between a culture solution and the cells, promote the growth and the differentiation of the mesenchymal stem cells, improve a transformation rate and enhance cell activity by virtue of the three-dimensional spatial structure of an alginic acid gel body.

The invention discloses a tissue culture and rapid propagation method of highly efficient induction of cluster buds of davidia involucrata. The method includes the specific steps: 1) selecting davidia involucrata branches as mother plants, wherein the davidia involucrata branches have robust growth, are free of pest and disease damage, have good characters and form lateral buds without leaf expansion, and soaking the davidia involucrata branches in a GA3 solution until the lateral buds of the davidia involucrata grow to 3 cm or above; 2) shearing the lateral buds, conducting disinfection, inoculating the lateral buds to a culture medium which contains Wpm, 1.2 mg / L<-1> of KT, 0.3 mg / L<-1> of NAA, 0.5 mg / L<-1> of 6-BA, 3.5 g / L<-1> of agar, 20 g / L<-1> of sucrose and 0.2 g / L<-1> of AC, and conducting cultivation for 18-22 days until bud points germinate and grow to form the cluster buds. By the use of a provided davidia involucrata cluster bud induction technology, the method is simple, the induction cycle is short, the propagation coefficient is high, and the method has a certain role in promoting the national level protection of rare and endangered plants.

The invention relates to a preparation method of an oral biofilm. The preparation method comprises the following steps: adding type I collagen protein into an acetic acid solution to prepare a collagen-acetic acid swelling solution; adding chondroitin sulfate and stirring to prepare a collagen-chondroitin sulfate serous fluid; carrying out vacuum freeze-drying; pressing to prepare a collagen composite film; spraying the collagen-chondroitin sulfate serous fluid between two layers of the collagen composite film, and carrying out vacuum freeze-drying; carrying out high temperature vacuum crosslinking; and sterilizing after crosslinking to obtain a collagen-chondroitin sulfate composite oral biofilm. The invention has the following beneficial effects: the oral biofilm prepared by the above method has good histocompatibility, suitable controllable degradability and absorbability, low antigenicity and good plasticity and mechanical property; the oral biofilm is used in oral operation; and the three-dimensional porous structure of the oral film provides a tissue engineering scaffold for repairing of wounded tissues, is beneficial to invasion of cells and differential growth and can induce reparative regeneration of defective tissues, and specific thickness of the biofilm can effectively prevent ingrowth of soft tissue.

The invention discloses resuscitation fluid applied to vitrified cryopreserved mesenchymal stem cells and belongs to the field of resuscitation fluid. The resuscitation fluid applied to the vitrified cryopreserved mesenchymal stem cells comprises 20% of human serum albumin, 0.8-1% of monosaccharide, 0.21-0.23% of a cell growth factor, 1.5% of N-2-hydroxyethylpiperazine-N'-2-ethane-sulphonic acid, 0.8-1.2% of non-essential amino acid, 0.08-0.12% of L-glutamine, 0.03-0.04% of beta-mercaptoethanol and normal saline. The resuscitation fluid applied to the vitrified cryopreserved mesenchymal stem cells has the characteristics of low cell toxicity on the mesenchymal stem cells, low cell toxicity damage and low permeability damage in a vitrified cryopreserved resuscitation process and increased cryopreserved cell resuscitation rate.

The invention discloses a planting method for Tibetan medicine scindapsus aureus, relates to cultivation for a Tibetan medicine, and belongs to the field of biotechnology. The planting method for Tibetan medicine scindapsus aureus is characterized by comprising the following steps of: in spring, performing division propagation, dividing the roots of scindapsus aureus into clusters, and each cluster comprising 3-4 pseudobulbs; planting the clusters in a matrix respectively, selecting the scindapsus aureus with 3-4 leaves and 3-5 roots which are 4-5 cm long; and planting the scindapsus aureus in the matrix, and culturing in the conditions of a humidity of 80% and a temperature of 20 DEG C. The planting method for Tibetan medicine scindapsus aureus has the beneficial effects that lots of scindapsus aureus can be obtained in a short time via small plants for eliminating free radicals, and promoting cell differentiation and the callus differentiation and growth of scindapsus aureus by virtue of plant hormones, thus radically solving the problems of the provenance shortage and short growth time of scindapsus aureus.

Disclosed herein are compositions of CPG15-2 and methods for treating conditions of excessive cell death, such as neurological conditions, using such compositions. Compounds that inhibit the activity of CPG15-2 are also disclosed herein for the treatment of conditions of undesirable cell survival, such as cancer.

The invention discloses elastic-cell recontouring facial filler composition gel and a preparation method thereof and particularly relates to the field of beauty injections. The elastic-cell recontouring facial filler composition gel comprises 80-120 parts of an injection solution, 15-21 parts of umbilical cord mesenchymal stem cells, 8-12 parts of vitamin A, 3-6 parts of vitamin C, 0.8-1.8 parts of sodium hyaluronate, 2-4 parts of seaweed extract, 0.4-0.8 part of alginate fiber, 1-2 parts of polylactic acid microspheres, and 1.5-2.5 parts of an excipient. With the umbilical cord mesenchymal stem cells improving cell regenerability, sodium hyaluronate improving thickness, vitamin C promoting proliferation and differentiation of epithelial cells, removing oxygen radicals and promoting regeneration of the skin of an injured part, vitamin A promoting the growth and differentiation of skin cells and promoting blood-producing function of the epidermal skin, and the seaweed extract stimulating fibrocytes to generate collagens and elastin, the elastic-cell recontouring facial filler composition gel can supplement water, preserve moisture, resist senility, soften skin, improve elasticity, revive the skin, maintain youth and ensure less dependence on gel.

Disclosed herein are compositions of CPG 15-2 and methods for treating conditions of excessive cell death, such as neurological conditions, using such compositions. Compounds that inhibit the activity of CPG 15-2 are also disclosed herein for the treatment of conditions of undesirable cell survival, such as cancer.

The invention discloses a nanometer compound nutrient solution preparation special for broiler chicken and a preparation method thereof. The nanometer compound nutrient solution disclosed by the invention contains 3%-15% of vitamins, 1%-8% of amino acid, 1%-6% of nucleotide, 1%-3% of prebiotics, 6%-30% of surfactant, 2%-12% of cosurfactant, 0.1%-0.5% of anti-bacteria preservative and the balance of purified water. A nanometer technology is adopted for compounding the nutrient substances, such as, vitamins, amino acid, nucleotide and prebiotics into a nanometer emulsion according to the requirement of the broiler chicken for the nutrient substances and the nanometer emulsion is a safe, efficient, stable and quality-controllable compound nutrient nanometer emulsion special for broiler chicken. The compound nutrient solution disclosed by the invention can improve the water solubility, dispersity and absorption rate of the nutrient substances, such as vitamins, and can thoroughly solve the problem of bioavailability of the nutrient substances, such as, lipid-soluble vitamins. A nutrition enriched solution which can be highly absorbed and utilized is supplied for the broiler chicken.

The invention discloses a 3D full-layer skin model, a culture medium for forming the 3D full-layer skin model and a preparation method of the 3D full-layer skin model. The culture medium comprises a protein preparation, and the protein preparation comprises the following components in percentage by mass: 0.1-99% of albumin, 0.1-99% of globulin and 0.1-99% of fibronectin. Serum is not needed in thegas-liquid phase process of preparing the 3D full-thickness skin model by combining the culture medium with pig type I collagen; the prepared 3D full-layer skin model is complete, the activity and the stability are remarkably improved, the application field of the type of model is expanded, and large-scale industrial production of the skin model is achieved.

The invention provides a drug-loaded degradable blood vessel anastomat. The drug-loaded degradable blood vessel anastomat is characterized by comprising main components which are symmetrically arranged; each main component comprises a clamping part, a puncture part and a matching hole, and the puncture parts and the matching holes are symmetrically distributed in the clamping parts respectively; and the puncture parts of the adjacent main parts are connected with the corresponding matching holes in a matching manner. Through controllable degradation, the risk caused by retention of the vascular anastomat is reduced, the strength of the puncture parts can be guaranteed through a pointed iron core, the anastomosis effect is guaranteed, and meanwhile, an iron base body is degradable, the degradation component ferrous ions or iron ions are beneficial to formation of hemoglobin, and blood transportation is facilitated; and growth and differentiation of vascular cells can be promoted throughdrug loading, anastomosis of blood vessels at anastomotic positions is accelerated, generation of spots is reduced, and tissue damage is reduced to the maximum extent.

The invention relates to a double-layer structure periosteum for diabetic bone repair and a preparation method thereof, belonging to the field of biomedical materials. Double-layer periosteum, the inner layer is chitosan / polyvinyl alcohol / calycosin blended in a certain proportion of porous structure sponge-like nanofiber layer, the outer layer is polydioxanone nanofiber layer, electrospun Composite preparation of silk technology. In the double-layer structure periosteum of the present invention, the outer layer has suitable mechanical properties, can effectively support the bone defect site, and prevent other soft tissues from growing in; the inner layer is loose and porous, suitable for the growth, attachment and reproduction of osteoblasts, And can play antibacterial antibacterial, hypoglycemic and other functions. The composite function of the inner and outer layers of the periosteum of the double-layer structure makes it beneficial to the growth of bone cells and the repair of bone tissue, and has the characteristics of simple preparation process and convenient operation.

The invention discloses a titanium alloy bone implant adopting a bio-coating structure and a preparation method of the titanium alloy bone implant. The titanium alloy bone implant comprises a titanium alloy matrix, a porous metal powder coating and a bio-coating, wherein the porous metal powder coating is uniformly distributed on the surface of the titanium alloy matrix and the bio-coating is uniformly distributed on the surface of the porous metal powder coating. The porous metal powder coating and the bio-coating are designed on the implant, multiple pores are formed in the surface of the implant by the aid of the porous metal alloy coating, the bio-coating enters the pores during coating to form closer connection between coatings, the frozen bio-coating forms tiny pores, the bio-coating is made of a resistance-free and easy-to-absorb material, thus, adhesion, proliferation and differentiation of cells are facilitated, regenerated cell tissue can be tightly combined with the porous titanium alloy coating after the bio-coating is completely degraded, the adhesion of regenerated tissue and the implant can be improved, and rejection reactions can be reduced.

The invention relates to the technical field of new materials and relates to a preparation method of a crosslinked chitosan / hyaluronic acid compound porous scaffold. According to the preparation method, natural biological materials such as chitosan and hyaluronic acid are used as raw materials and an organic silane coupling agent KH-560 is used as a crosslinking agent, and the crosslinked chitosan / hyaluronic acid compound porous scaffold which is made of purely natural biological materials and is excellent in mechanical property is prepared adopting a freeze-drying method. The crosslinked chitosan / hyaluronic acid compound porous scaffold prepared by adopting the preparation method has the characteristics of high porosity, high pores strength, large pores diameter and the like, is of an open type pore structure that small pores are contained in large pores, and is suitable for planting cells on the surface of the scaffold, wherein the pore diameter is 100-300 mu m and porosity is more than 90%. Compared with a chitosan scaffold, the crosslinked chitosan / hyaluronic acid compound porous scaffold has the advantages that the mechanical property is better; proliferation and differentiation effect of the cells on the surface of the porous scaffold is better due to the addition of the organic silane coupling agent, and affinity to biological cells is better.