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A double-layer periosteum for diabetic bone repair and its preparation method

A double-layer structure, diabetic bone technology, applied in prosthesis, tissue regeneration, medical science, etc., can solve the problems of lack of periosteum regeneration structure and function, achieve excellent biodegradability and absorbability, good antibacterial ability, excellent degradability effect

Active Publication Date: 2021-08-03
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: the existing periosteum scaffold material only has a certain effect of promoting bone regeneration, and lacks the structure and function required for periosteum regeneration

Method used

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  • A double-layer periosteum for diabetic bone repair and its preparation method
  • A double-layer periosteum for diabetic bone repair and its preparation method
  • A double-layer periosteum for diabetic bone repair and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Step 1: Preparation of inner layer:

[0031] Add chitosan (CS) and polyvinyl alcohol (PVA) to 20% acetic acid solution at a mass percentage of 10:90 to prepare a 10% solution, and stir in a water bath at a temperature of 90°C for more than 12 hours , after the obtained solution was allowed to stand for cooling, 5 μL of calycosin with a concentration of 40 mmol / L was added, and stirred at room temperature for 6 hours to obtain the electrospinning solution of the inner layer;

[0032] Using an electrospinning device, control the receiving distance to 16cm, the voltage to 18KV, the liquid flow rate to 0.3ml / h, and a 20G flat needle to obtain the inner nanofiber membrane by electrospinning under the condition of a relative humidity of 30%;

[0033] Select 25% glutaraldehyde solution to crosslink the obtained nanofiber membrane for 1 hour, then dry it in a vacuum oven at 50°C for 30 minutes, cut the crosslinked nanofiber membrane into pieces, and add a volume ratio of 20:80...

Embodiment 2

[0039] Step 1: Preparation of inner layer:

[0040]Add chitosan (CS) and polyvinyl alcohol (PVA) to 20% acetic acid solution at a mass percentage of 20:80 to prepare a 10% solution, and stir in a water bath at a temperature of 90°C for more than 12 hours After the obtained solution was allowed to stand and cool, 7uL of calycosin with a concentration of 40mmol / L was added, and stirred at room temperature for 6h to obtain the electrospinning solution of the inner layer;

[0041] An electrospinning device is used to control the receiving distance of 17cm, voltage of 19KV, liquid flow rate of 0.45mL / h, 20G flat needle, and electrospinning under the condition of relative humidity of 30% to obtain the nanofiber film of the inner layer;

[0042] Select the glutaraldehyde solution with a concentration of 25% to carry out crosslinking treatment on the obtained nanofiber membrane for 1 hour, then dry it in a vacuum oven at 50°C for 30 minutes, cut the crosslinked nanofiber membrane into...

Embodiment 3

[0048] Step 1: Preparation of inner layer:

[0049] Add chitosan (CS) and polyvinyl alcohol (PVA) to the acetic acid solution with a concentration of 20% at a mass percentage of 30:70 to prepare a solution with a concentration of 10%, and stir in a water bath at a temperature of 90°C for more than 12 hours , after the obtained solution was allowed to stand for cooling, 9 μL of calycosin with a concentration of 40 mmol / L was added, and stirred at room temperature for 6 hours to obtain the electrospinning solution of the inner layer;

[0050] Using an electrospinning device, control the receiving distance of 18cm, voltage of 20KV, liquid flow rate of 0.6ml / h, 20G flat needle, and obtain the inner nanofiber membrane by electrospinning under the condition of relative humidity of 30%;

[0051] Select the glutaraldehyde solution with a concentration of 25% to crosslink the obtained nanofiber membrane for 1 hour, then dry it in a vacuum oven at 50°C for 30 minutes, cut the crosslinke...

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Abstract

The invention relates to a double-layer structure periosteum for diabetic bone repair and a preparation method thereof, belonging to the field of biomedical materials. Double-layer periosteum, the inner layer is chitosan / polyvinyl alcohol / calycosin blended in a certain proportion of porous structure sponge-like nanofiber layer, the outer layer is polydioxanone nanofiber layer, electrospun Composite preparation of silk technology. In the double-layer structure periosteum of the present invention, the outer layer has suitable mechanical properties, can effectively support the bone defect site, and prevent other soft tissues from growing in; the inner layer is loose and porous, suitable for the growth, attachment and reproduction of osteoblasts, And can play antibacterial antibacterial, hypoglycemic and other functions. The composite function of the inner and outer layers of the periosteum of the double-layer structure makes it beneficial to the growth of bone cells and the repair of bone tissue, and has the characteristics of simple preparation process and convenient operation.

Description

technical field [0001] The invention relates to a double-layer structure periosteum for diabetic bone repair and a preparation method thereof, belonging to the field of medical materials. Background technique [0002] At present, diabetes has become one of the chronic diseases with the highest incidence rate, and the number of diabetic patients with bone defects caused by factors such as trauma and inflammation is also increasing. Bone defects are caused by trauma, infection or tumor, surgical debridement of osteomyelitis, and various congenital diseases. Bone defects are relatively common clinically. Due to the increasing population aging phenomenon in my country in recent years, as well as sports injuries, congenital deformities or accident trauma, etc. will cause large area bone defects. The prevalence of osteopenia and osteoporosis in diabetic patients is as high as 48-72%. Hyperglycemia can change the biomineralization process of osteoblasts and enhance mineralizatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/16A61L27/18A61L27/20A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/16A61L27/18A61L27/20A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/216A61L2300/404A61L2300/408A61L2430/02C08L5/08C08L29/04C08L67/04
Inventor 张佩华高原付少举郭虹孙玮沈为王峰
Owner DONGHUA UNIV
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