120 results about "Calycosin" patented technology

The invention discloses a six-flavor hematinic capsule, its quality control method and an application thereof. The six-flavor hematinic capsule is prepared by adding auxiliary materials into raw materials consisting of Chinese angelica, Ligusticum wallichii, Radix Astragali, prepared rehmannia root, lithospermum and white peony root. According to the quality control method of the six-flavor hematinic preparation, whether the six-flavor hematinic capsule contains white peony root, Chinese angelica, Radix Astragali, prepared rehmannia root, lithospermum, Ligusticum wallichii and components is identified by thin layer chromatography; by high-performance liquid chromatography, in vitro dissolution behavior of the six-flavor hematinic capsule is determined, and effective component groups are identified by fingerprint; verbascoside and calycosin glucoside are used as reference substances to simultaneously determine contents in the six-flavor hematinic capsule; and the content of a volatile component ligustilide in the six-flavor hematinic capsule is determined by a gas chromatographic method. The method provided by the invention is simple to operate, is accurate and advanced, has good linear relation, reappearance, precision, stability and recovery rate, can be adopted to effectively control product quality and guarantee curative effect of the product. The invention also provides an application in the preparation of a medicine for treating eye damage and blurred vision.

The invention relates to a method for determining fingerprint chromatography of radix astragali and ligusticum wallichii extract products, which comprises the following steps: 1)taking Radix Astragali and Ligusticum wallichii extract product fine powder, preciously weighing, adding methanol, performing ultrasonic extraction, filtering and drying a filtrate, dissolving residue by ethanol and metering volume, filtering by a filter membrane, taking a subsequent filtrate to obtain a tested object solution; 2)taking a ferulic acid reference substance, a senkyunolide I reference substance, a calycosin glucoside reference substance, an ononin reference substance, a calycosin reference substance and a fermlononetin reference substance, preciously weighing, respectively adding methanol to prepare a reference substance solution; and 3)respectively and preciously absorbing the tested object solution and the reference substance solution, and injecting a high efficiency liquid chromatography for determining to obtain the fingerprint chromatography of radix astragali and ligusticum wallichii extract products. The method has active effect for guiding clinical medication and effective feeding guidance to the bulk drugs during the production process, and ensuring the reliable quality; the method has the advantages of convenient and fast operation, so that similarity result can be obtained, the quality of the traditional Chinese medicinal materials radix astragali and ligusticum wallichii extract products can be evaluated, and the result is objective and accurate.

The present invention discloses a UPLC-MS quantitative method for the contents of twelve components in a traditional Chinese medicine composition preparation, specifically determination of the contents of calycosin-7-O-beta-D-glucoside (1), isoquercitrin (2), narirutin (3), hesperidin (4), ginsenoside Re (5), ginsenoside Rg1(6), periplocoside (7), ginsenoside Rf (8), ginsenoside Rb1 (9), astragaloside (10), ginsenoside Rd (11) and periplocin H1 (12) in the traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine composition preparation component detection. The determining method of the present invention has characteristics of short period, good reproducibility and high sensitivity.

The invention discloses a quality inspection method of Tongkang tablets. The Tongkang tablet is prepared from the following raw materials: 80.5g of astragalus membranaceus, 53.5g of white atractylodes rhizome, 27g of divaricate saposhnikovia root, 80.5g of Chinese yam, 6.5g of dried tangerine or orange peel, and 80.5g of oyster. The quality inspection method comprises the following steps of morphological identification, inspection, fingerprint determination and content determination, wherein the identification is characterized by adopting a thin layer chromatography to identify the astragalus membranaceus, the white atractylodes rhizome, the divaricate saposhnikovia root and the dried tangerine or orange peel in the Tongkang tablet; the fingerprint determination is characterized by determining a fingerprint of a hesporidin component in the Tongkang tablet through a high performance liquid chromatography; the content determination is characterized by adopting the high performance liquid chromatography to carry out content determination on prim-o-glucosylcimifugin, calycosin-7-glucoside, 5-O-methylvisammioside and hesporidin in the Tongkang tablet at the same time. The quality inspection method provided by the invention has the advantages of quickness, stability, accuracy and the like, provides a standard inspection method for production and use of the Tongkang tablets, and can effectively ensure the quality of the Tongkang tablets during the production and use processes, so that the pharmaceutical effectiveness is ensured.

The invention provides a building method for a fingerprint of a radix astragali seu hedysari pill with the effect of reinforcing middle warmer. The building method comprises the following steps: 1) preparing a reference methanol solution from a proper amount of calycosin, fermlononetin and paeoniflorin; 2) preparing a test article methanol solution from the radix astragali seu hedysari pill with the effect of reinforcing middle warmer; 3) injecting 10muml of the reference methanol solution and the test article methanol solution into an ultra performance liquid chromatograph, and performing gradient elution under the condition of ultra performance liquid chromatography (UPLC) to obtain a fingerprint, wherein the operating conditions of the liquid chromatograph are as follows: a moving phase is prepared from acetonitrile and a 0.1-percent methanoic acid aqueous solution, the flow speed of the moving phase is 0.3ml/min, the column temperature is 30 DEG C, and the detection wavelength is 254nm. The built fingerprint can clearly reflect the peak outlet positions of feature peaks of effective components in the radix astragali seu hedysari pill with the effect of reinforcing middle warmer, and can be used for controlling the quality of the radix astragali seu hedysari pill with the effect of reinforcing middle warmer.

The invention relates to a method for preparing milkvetch root fried with honey. The method comprises the following steps: moistening raw milkvetch root and honey until the honey water is totally absorbed by the raw milkvetch root, frying the moistened milkvetch root at the temperature of 220-240 DEG C, thereby obtaining the milkvetch root fried with honey. According to the preparation method provided by the invention, the milkvetch root is moistened and fried at a high temperature, so that the heating temperature and time of the milkvetch root can be controlled, and the preparation process is controllable. According to the milkvetch root fried with honey prepared by the method, the content of astragaloside is 6.56-11.21mg/g, the content of calycosin-7-glucoside is 3.95-8.15mg/g, and the content of the components is far higher than that in the prior art.

The invention provides applications of an isoflavone compound in preparing antiviral medicaments. The isoflavone compound provided by the invention is calycosin, and has obvious AIDS (Acquired Immune Deficiency Syndrome) virus and coxsakie virus resistant activities. The isoflavone compound provided by the invention as an active component is used for preparing medicaments for preventing or treating viral diseases.

The invention discloses a high efficient sewage cleaning agent, which comprises the following components in parts by weight: 40 to 60 parts of diatomite, 15 to 25 parts of montmorillonite, 5 to 15 parts of bentonite, 1 to 3 parts of nutrients, 6 to 10 parts of microbial bacteria, and 0.4 to 0.8 part of calycosin; wherein the microbial bacteria comprise ammonifying bacteria, nitrifying bacteria, denitrifying bacteria, phosphor eating bacteria, and actinomycetes; and the nutrients comprise chicken manure and horse manure. The provided high efficient sewage cleaning agent can degrade organic substances in sewage high efficiently and is capable of prominently reducing the COD value. The cleaning agent comprises calycosin, which can prominently promote the degradation of organic substances by microbial bacteria and obviously improves the cleaning effect.

The invention belongs to the field of medicines, and particularly relates to a cyclophosphamide-containing pharmaceutical composition, and an application thereof in treatment for cancer, in particular for breast cancer. The pharmaceutical composition is composed of cyclophosphamide, salidroside and calycosin-7-oxy-beya-D-glucopyranoside, wherein the molar ratio of cyclophosphamide to salidroside to calycosin-7-oxy-beya-D-glucopyranoside is (20-40): (15-25): (5-15). The pharmaceutical composition disclosed by the invention is capable of reducing the toxic and side effects of cyclophosphamide and remarkably enhancing the curative effect, provides a new thought and strategy for treatment of breast cancer, and has important clinical significance and wide application prospect.

The present invention relates to a pharmaceutical composition for preventing and treating diabetic complications, mainly referring to diabetic nephropathy, and the pharmaceutical composition comprises one or both of calycosin and calycosin-7-O-β-D-glucoside as 0.1˜99.5% by weight based on the total weight of the composition, and the conventional drug carrier. The pharmaceutical composition could significantly prevent and treat diabetic nephropathy, with the convenience of quality control and administration, which provides a new drug candidate for patients with diabetic nephropathy.

The invention discloses a pharmaceutical composition for treating diabetes mellitus in earlier stage. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 5-15 parts of epiberberine, 10-20 parts of coptisine, 15-25 parts of palmatine, 20-35 parts of berberine, 10-15 parts of astragaloside, 0-10 parts of astragalus saponin II, 0-15 parts of astragalus saponin III, 0-10 parts of astragalus saponin I, 20-30 parts of calycosin-7-glucoside, 0-20 parts of formononetin and 0-18 parts of ononin. The pharmaceutical composition provides a new choice for the treatment of the diabetes mellitus in the earlier stage, changes the status quo of the lack of effective prevention and treatment medicines of the diabetes mellitus in the earlier stage, can significantly improve the clinical symptoms of the diabetes mellitus in the earlier stage, significantly reduces blood glucose and glycosylated hemoglobin levels, regulates glucose metabolism, and significantly prevents or delays the progress of the diabetes mellitus in the earlier stage.

The invention provides a method for analyzing all components of an angelica sinensis six-yellow decoction, and belongs to the technical field of pharmaceutical analysis. An ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technology is adopted to detect an angelica sinensis six-yellow decoction sample, and 15 chemical reference substances are adopted at the same time: baicalin, wogonoside, berberine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, phellodendrine hydrochloride, astragaloside, calycosin-7-glucoside, ferulic acid, chlorogenic acid, ligustilide, verbascoside, baicalein and wogonin are adopted to confirm a detection result of the angelica sinensis six-yellow decoction andclearly identify 68 components; comprehensive analysis of the components of the angelica sinensis six-yellow decoction is realized, and the reliability of quality control is improved.

The invention relates to a fingerprint construction method and a quality detection method for a Radix Astragali and Chinese date oral liquid. The fingerprint construction method comprises the following steps: taking protocatechuic acid, calycosin-7-glucoside, ononin, calycosin, genistein and formononetin as reference substances, and carrying out gradient elution with 50% aqueous acetonitrile solution-0.2% aqueous phosphoric acid solution as a mobile phase; and carrying out segmented wavelength detection, and recording and obtaining the fingerprint. The quality detection method comprises the following steps: constructing standard fingerprints of 10 or more batches of the Radix Astragali and Chinese date oral liquid through the fingerprint construction method, and carrying out consistence comparison on the standard fingerprints and the fingerprint of a sample to be detected in order to detect the quality of the sample to be detected. Compared with the prior art, the methods have the advantages of high specificity and stability, realization of full, accurate and quick detection of the quality of the finished or semi-finished product of the Radix Astragali and Chinese date oral liquid, and suitableness for large-scale production and application.