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Method for rapidly detecting index composition in Chinese herbal medicine compound preparation

The technology of compound preparation and traditional Chinese medicine is applied in the field of rapid detection of the content of index components of the compound preparation of traditional Chinese medicine Fangji Huangqi Decoction. Avoid time and reagent consuming effects

Active Publication Date: 2015-03-18
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

The quality of Fangji Huangqi Decoction can not be fully and effectively measured by the detection method described

Method used

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  • Method for rapidly detecting index composition in Chinese herbal medicine compound preparation
  • Method for rapidly detecting index composition in Chinese herbal medicine compound preparation
  • Method for rapidly detecting index composition in Chinese herbal medicine compound preparation

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Embodiment 1

[0029] In this embodiment, the method for rapidly detecting the content of index components in the compound preparation of traditional Chinese medicine according to the following steps

[0030] (1) Preparation of reference substance solution: get respectively an appropriate amount of fangchinoline, tetrandrine powder, calycosin glucoside, liquiritin and glycyrrhizic acid reference substance, accurately weighed, add methanol to make every 1ml containing fangchinoline 100ug, A mixed solution of tetrandrine 100ug, calycosin glucoside 50ug, liquiritin 100ug and glycyrrhizic acid 100ug was used as the reference solution;

[0031] (2) Preparation of Fangji Astragalus Decoction Extraction Concentrate: Fangji, Astragalus and other medicines were first reflux extracted twice with 10 times the amount of 70% ethanol, each time for 1.5h; then decocted twice with 12 times the amount of water, each time for 1.5h ( Referring to the prior art: New Drugs of Traditional Chinese Medicine and Cli...

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Abstract

The invention relates to the field of detection on traditional Chinese medicine quality, relates to a method for rapidly detecting index composition in Chinese herbal medicine compound preparation, and especially relates to a rapid detection method for index composition in a Chinese herbal medicine compound preparation radix-stephaniae-tetrandrae radix-astragali soup. According to the method, methanol with the concentration of 50% is employed for ultrasonic extraction, and an HPLC-DAD process is applied to simutaneously determine the content of fangchinoline, tetrandrine, calycosin-7-glucoside, liquiritin and glycyrrhizic acid in radix stephaniae tetrandrae, radix astragali and radix glycyrrhizae in the radix-stephaniae-tetrandrae radix-astragali soup at one time. The method solves the problem that quality control is simultaneously performed on stephaniae tetrandrae, radix astragali and radix glycyrrhizae in the radix-stephaniae-tetrandrae radix-astragali soup, and avoids time and reagent waste caused by multiple detection on the five index compositions. The method is simple in operation and high in working efficiency and precision.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicines, and relates to a method for rapidly detecting the content of index components in compound Chinese medicine preparations, in particular to a method for rapidly detecting the content of index components in Fangji Huangqi Decoction, a compound Chinese medicine preparation. Background technique [0002] Fangji Huangqi Decoction, a compound preparation of traditional Chinese medicine, comes from "Synopsis of the Golden Chamber". Zhang Zhongjing's "Synopsis of the Golden Chamber·Water Qi Disease" has skin and geomantic o For those, Fangji Huangqi Decoction is the main one.” This prescription is designed for rheumatism on the surface, deficiencies of the body are not solid, and symptoms include floating pulse, heavy body, sweating and aversion to wind. The whole prescription is mainly composed of Fangji, Astragalus, Atractylodes Rhizoma Atractylodes Rhizome and Licorice ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 王拥军施杞刘书芬隋璐梁倩倩李晨光崔学军杨燕萍唐德志王成龙舒冰王晶贾友冀王洪伸李晓峰陈岩李金龙卢盛赵永见杨龙薛纯纯
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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