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Fingerprint spectrum detection method of medicinal preparation

A detection method and fingerprint technology, applied in the field of detection and analysis of pharmaceutical ingredients, can solve the problems that cannot be used to evaluate the internal quality of Chinese patent medicines, and the operation steps are cumbersome

Pending Publication Date: 2020-11-27
SHAANXI BUCHANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned content detection method also has cumbersome operation steps, and the active ingredients selected in the above-mentioned quality standard system cannot be used to evaluate the intrinsic quality of the Chinese patent medicine.

Method used

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  • Fingerprint spectrum detection method of medicinal preparation
  • Fingerprint spectrum detection method of medicinal preparation
  • Fingerprint spectrum detection method of medicinal preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: The detection method of drug fingerprint of the present invention:

[0057] 1 Instruments and reagents

[0058] ACQUITYH-CLASS type ultra-high performance liquid chromatography system (Waters, USA), including binary ultra-high pressure solvent system, FTN automatic sample injection manager, PDA detector and Empower 3 chromatography workstation; Sartorius CPA225D type 1 / 100,000 electronic analysis Balance (Sartorius Scientific Instrument Co., Ltd., Germany); KQ-300DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0059] Actascoside (batch number: 111920-201505, purity: 97.1%) was purchased from China National Institutes for Food and Drug Control, Sangbaritin A (batch number: MUST-17060301, purity: 99.42%), hydroxysafflor yellow A (batch number: MUST-16092910, purity: 99.88% HPLC), amygdalin (lot number: MUST-17042810, purity: 99.28% HPLC), paeoniflorin (lot number: MUST-17031901, purity: 99.30%), rosmarinic acid (lot number: MUST-170405...

Embodiment 2

[0100] Embodiment 2: Assay of pharmaceutical preparation of the present invention:

[0101] 2.1 Content determination

[0102] 2.1.1 Chromatographic conditions

[0103] Using ACQUITY BEH C18 (50mm×2.1mm, 1.7μm) chromatographic column, the mobile phase is 0.2% formic acid-water solution (A)-acetonitrile solution (B), the gradient elution program is 0~2min, 2%B; 2~7min, 2 %~14%B; 7~9min, 14%~15%B; 9~11min, 15%~16%B; 11~14min, 16%~35%B; 14~16min, 35%B; 16~21min , 35%~70%B; 21~23min, 70%B; 23~28min, 70%~100%B; 28~30min, 100%B; 30~32min, 100%~2%B; after running for 5min; The volume flow rate is 0.2ml / min; the column temperature is 35°C; the detection wavelength is 254nm; the injection volume is 5μl.

[0104] 2.1.2 Preparation of mixed reference solution

[0105] Accurately weigh the appropriate amount of mulberry glycoside A, hydroxysafflower yellow A, paeoniflorin, ferulic acid, verbascoside, rosmarinic acid, salvianolic acid B, formononetin and tanshinone IIA reference subst...

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Abstract

The invention provides a fingerprint spectrum detection method of a medicinal preparation. According to the detection method, an HPLC-DAD wavelength switching method is adopted to simultaneously determine a plurality of effective components such as mulberroside A, hydroxysafflor yellow A, paeoniflorin, ferulic acid, calycosin-7-glucoside, rosmarinic acid, salvianolic acid B, formononetin and the like in the medicinal preparation; the sensitivity and the accuracy of the detection method are greatly enhanced, so that the comprehensiveness of quality evaluation of the medicinal preparation is guaranteed.

Description

technical field [0001] The invention relates to the field of detection and analysis of pharmaceutical ingredients, in particular to a fingerprint detection method for pharmaceutical preparations. Background technique [0002] The representative drug of the pharmaceutical preparation of the present invention is Naoxintong Capsule, which is composed of astragalus, red peony root, salvia miltiorrhiza, angelica, chuanxiong, peach kernel, safflower, spatholobus, achyranthes bidentata, cassia twig, mulberry branch, Composed of sixteen herbs such as earthworm, scorpion, and leech. The pharmaceutical preparation of the present invention is an exclusive product of Shaanxi Buchang Pharmaceutical Co., Ltd., which is a large variety of traditional Chinese medicine for treating cardiovascular and cerebrovascular diseases. It is used for apoplexy caused by qi deficiency and blood stagnation, meridian stasis, hemiplegia, numbness of limbs, crooked mouth and eyes, strong tongue, lingering ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675G01N2030/047
Inventor 唐志书刘峰刘妍如许刚谢伟宋忠兴陈衍斌张健苏瑞赵超
Owner SHAANXI BUCHANG PHARMA
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