32 results about "Senkyunolide I" patented technology

The invention discloses a separation and preparation method of ligustilide. The method comprises the following steps by the technology of high-speed countercurrent chromatography. The method comprises the following steps: extracting ligusticum wallichii by a solution to obtain a ligusticum wallichii extract; extracting the ligusticum wallichii extract by ethyl acetate to obtain an ethyl acetate part; separating the ethyl acetate parts by high-speed countercurrent chromatography at the same time to obtain a plurality of ligustilide compounds which are respectively senkyunolide N, senkyunolide R, senkyunolide S and senkyunolide I through structural identification, wherein the purity is over 98%. The separation and preparation method of ligustilide disclosed by the invention has the advantages of simplicity in operation, few use level of solvent, no sample adsorption, loss and pollution, high efficiency and the like.

The invention discloses a new medical application of senkyunolide I. Inventor adopts animal model, has studied the effect of chuanxionglide I on rat plasma and brain tissue monoamine transmitter, the influence of chuanxionglide I on rat plasma and brain tissue NO and the effect of chuanxionglide I on rat plasma and brain tissue NO. The impact of mouse pain threshold, subsequent examples and pharmacological results thereof will confirm that Ligustin I has the ability to regulate monoamine transmitters, especially the content of 5-hydroxytryptamine (5-HT) in blood plasma and brain tissue, so Ligustilide I can be used to prepare medicines for preventing and treating depression, migraine, and other diseases related to serotonergic system.

The invention discloses a method for preparing senkyunolide I from extract of Chinese angelica, which comprises the following steps: (1) purifying by using macroporous resin; (2) purifying by extraction; and (3) purifying by chromatography. In the invention, the preparation process of the senkyunolide I is simplified, and the yield is increased to a great extent. In addition, the method prepares the senkyunolide I by using the extract of Chinese angelica which is a traditional Chinese medicine instead of Szechuan lovage rhizome which is an original source plant. Therefore, the method can be used in the industrial preparation of senkyunolide I.

The invention discloses a preparation method of ligustilide I, which belongs to the technical field of preparation of traditional Chinese medicine components. The method uses the extract containing ligustilide as a raw material and mixes it with potassium hydrogen persulfate compound salt in an alkaline aqueous solvent After the heating reaction, the reaction solution is extracted and separated by column chromatography to obtain the pure product of ligustilide I; the extract containing ligustilide in the present invention is used as a raw material, and Ligusticum ligusticum is prepared by a method combining chemical transformation and chromatographic separation. The lactone Ⅰ has the characteristics of simple process, less solvent consumption and high yield, and is suitable for the large-scale preparation of high-purity lactone Ⅰ.

The invention relates to a microwave-assisted extraction method of three lactone compounds in rhizoma chuanxiong and belongs to the field of extraction separation. The microwave-assisted extraction method includes: allowing an extracting agent to contact with rhizoma chuanxiong powder, wherein the extracting agent is a deep eutectic solvent formed by choline chloride and acetic acid, propionic acid or ethylene glycol according to the mole ratio of 1:1-1:5. The microwave-assisted extraction method has the advantages that ligustilide, senkyunolide I and senkyunolide H in the rhizoma chuanxiong powder can be extracted, the extraction efficiency of the lactone compounds can reach 180mg / L, and the method is high in extraction efficiency, high in selectivity, mild in operation condition and environmentally friendly.

The invention relates to a method for determining fingerprint chromatography of radix astragali and ligusticum wallichii extract products, which comprises the following steps: 1)taking Radix Astragali and Ligusticum wallichii extract product fine powder, preciously weighing, adding methanol, performing ultrasonic extraction, filtering and drying a filtrate, dissolving residue by ethanol and metering volume, filtering by a filter membrane, taking a subsequent filtrate to obtain a tested object solution; 2)taking a ferulic acid reference substance, a senkyunolide I reference substance, a calycosin glucoside reference substance, an ononin reference substance, a calycosin reference substance and a fermlononetin reference substance, preciously weighing, respectively adding methanol to prepare a reference substance solution; and 3)respectively and preciously absorbing the tested object solution and the reference substance solution, and injecting a high efficiency liquid chromatography for determining to obtain the fingerprint chromatography of radix astragali and ligusticum wallichii extract products. The method has active effect for guiding clinical medication and effective feeding guidance to the bulk drugs during the production process, and ensuring the reliable quality; the method has the advantages of convenient and fast operation, so that similarity result can be obtained, the quality of the traditional Chinese medicinal materials radix astragali and ligusticum wallichii extract products can be evaluated, and the result is objective and accurate.

The invention provides a content detection method for determining effective components in a longshengzhi capsule by an HPLC-QQQ / MS method. The chromatograph condition is that the chromatographic column takes a octadecyl-bonded silica gel column as a filling agent, 0.1% formic acid-water as a mobile phase A, 0.1% formic acid-acetonitrile as a mobile phase B, and the volume ratio of the mobile phaseA and the mobile phase B is 80% to 0%:20 to 100%, so that gradient elution is performed. According to the content detection method for determining the effective components in the longshengzhi capsuleby the HPLC-QQQ / MS method, the effective substance components of traditional Chinese medicines such as astragaloside A, hydroxysafflor yellow A, calycosin 7-o-glucoside, calycosin, ferulic Acid, syringin, n-butylphthalide, ligustilide, senkyunolide A, senkyunolide I, senkyunolide H, ligustrazine, isofraxidin, dehydrocostus lactone, amygdalin, syringin E, paeoniflorin, oxypaeoniflora, benzoylpaeoniflorin, etc., in the longshengzhi capsule can be determined. The method has the advantages of being simple in operation method, high in sensitivity and accurate in content.