A hplc-qqq/ms method for determining the content of active ingredients in Longshengzhi Capsules

Abstract

The invention provides a content detection method for determining effective components in a longshengzhi capsule by an HPLC-QQQ / MS method. The chromatograph condition is that the chromatographic column takes a octadecyl-bonded silica gel column as a filling agent, 0.1% formic acid-water as a mobile phase A, 0.1% formic acid-acetonitrile as a mobile phase B, and the volume ratio of the mobile phaseA and the mobile phase B is 80% to 0%:20 to 100%, so that gradient elution is performed. According to the content detection method for determining the effective components in the longshengzhi capsuleby the HPLC-QQQ / MS method, the effective substance components of traditional Chinese medicines such as astragaloside A, hydroxysafflor yellow A, calycosin 7-o-glucoside, calycosin, ferulic Acid, syringin, n-butylphthalide, ligustilide, senkyunolide A, senkyunolide I, senkyunolide H, ligustrazine, isofraxidin, dehydrocostus lactone, amygdalin, syringin E, paeoniflorin, oxypaeoniflora, benzoylpaeoniflorin, etc., in the longshengzhi capsule can be determined. The method has the advantages of being simple in operation method, high in sensitivity and accurate in content.

Description

technical field [0001] The invention relates to the field of chemical analysis and detection of Chinese patent medicines, in particular to an HPLC-QQQ / MS method for determining the content of active ingredients in Longshengzhi Capsules. Background technique [0002] Longshengzhi capsule is exclusively produced by Shaanxi Buchang Pharmaceutical Co., Ltd. The drug is composed of astragalus, leech, chuanxiong, angelica, peach kernel, safflower, red peony, woody, calamus, earthworm, mulberry and eleuthero A compound preparation composed of 12 traditional Chinese medicines has the effects of invigorating qi, activating blood circulation, removing blood stasis and dredging collaterals. Pre-marketing clinical studies have shown that the total effective rate of Longshengzhi Capsules for cerebral infarction can reach 91.7%, and the improvement rate for common TCM syndromes of stroke sequelae is above 80%. After being launched on the market, it is widely used in the treatment of cere...

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Problems solved by technology

[0003] At present, there are relatively many clinical studies on Longshengzhi Capsules, and the clinical effect is remarkable. However, the research on its chemical composition has not been reported, and its material basis is still unclear. Therefore, the determination of the chemical composition of Longshengzhi Capsules can effectively evaluate the quality of compound preparations. Whether it is to ensure the safety and effectiveness of medication, or to accurately and comprehensively evaluate the quality of medicinal materials, it is very necessary and meaningful

At present, the literature reports only use astragaloside IV as the quality control index of Longshengzhi Capsules, and the pharmacological effects of traditional Chinese medicine are the result of the synergistic effect of multiple active ingredients. Or a few indicators can not reflect the quality of traditional Chinese medicine more comprehensively, quantitative analysis of multi-component active ingredients is the most direct and necessary method for quality control of traditional Chinese medicine

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