The invention discloses a quick qualitative and quantitative method for
oligosaccharide in
breast milk. The quick qualitative and quantitative method mainly includes steps of 1, pretreating samples, to be more specific, removing fat and proteins from 150-250 micro-l of
breast milk to obtain ultimate supernatant, adding ultra-pure water into the supernatant and diluting the supernatant to obtain the loaded samples; 2, establishing standard curves for standard substances by the aid of ultrahigh-performance liquid
chromatography and
mass spectrometry; 3, separating different components of the
oligosaccharide in the
breast milk in the loaded samples by the aid of ultrahigh-performance liquid
mass spectrometry and carrying out quantitative analysis by the aid of
mass spectrometry combined with the standard curves so as to obtain the content of the
oligosaccharide in the breast milk. The ultrahigh-performance liquid
chromatography is implemented by the aid of amino chromatographic columns with the sizes of 2.1*100 mm and 1.7 micrometers, 8-10 mmol / L of
ammonium formate solution (A) and
acetonitrile (B), and the
ammonium formate solution (A) and the
acetonitrile (B) are used as mobile phases;
gradient elution programs are carried out by the aid of 95%-75% of B for 0-10 min or are carried out by the aid of 75% of B for 10-15 min or are carried out by the aid of 75%-65% of B for 15-20 min or are carried out by the aid of 65%-10% of B for 20-21 min or are carried out by the aid of 10% of B for 21-24 min or are carried out by the aid of 10%-95% of B for 24-25 min or are carried out by the aid of 95% of B for 25-35 min; the flow rates are 0.3 mL / min, and the column temperatures are 40-60 DEG C. The quick qualitative and quantitative method has the
advantage that 12 types of oligosaccharide in the breast milk can be quickly detected by the aid of the quick qualitative and quantitative method and can be quantified by the aid of the quick qualitative and quantitative method.