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Preparation of senkyunolide I and senkyunolide H from ligusticum wallichii extract via high-speed countercurrent chromatography

A kind of high-speed countercurrent chromatography, the technology of Chuanxiong extract, which is applied in the field of preparing Yangchuanxiong lactone I and H, can solve the problems of low yield, complicated preparation means and the like, and achieve the effect of simplifying the preparation process and improving the yield

Inactive Publication Date: 2014-07-02
SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is to provide a method for preparing chuanxionglide I and H from the extract of Rhizoma Chuanxiong, to solve the technical problems of cumbersome preparation means and extremely low yield in the prior art

Method used

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  • Preparation of senkyunolide I and senkyunolide H from ligusticum wallichii extract via high-speed countercurrent chromatography
  • Preparation of senkyunolide I and senkyunolide H from ligusticum wallichii extract via high-speed countercurrent chromatography

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Embodiment 1

[0016] Embodiment 1 The preparation of Liguscaractone I and H

[0017] 1. Sample: Ligusticum chuanxiong extract, refer to the patented method for preparing chuanxionglide I from the extract of chuanxiong (Feng Yi, Xu Desheng, Ruan Kefeng, Liang Shuang. Method for preparing chuanxionglide I from chuanxiong . A[P]. 2009-12-24.) Extracted from Rhizoma Chuanxiong.

[0018] 2. Instrument: TBE-300A high-speed countercurrent chromatograph, Shanghai Tongtian Biotechnology Co., Ltd.

[0019] 3. method:

[0020] As described in step (1) of patent claim 1, the extract of Rhizoma Chuanxiong was ultrasonically dissolved with 100 mL of the lower phase solvent (the concentration of the solution is equivalent to 10 g·mL of the crude drug -1 ), take n-butanol, acetic acid, and water, mix them according to the ratio of 4:1:5, let them stand in a separating funnel for stratification, separate the upper and lower phases and ultrasonically for 20 min, take the upper phase and fill the high-s...

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Abstract

The invention discloses preparation of senkyunolide I and senkyunolide H from ligusticum wallichii extract via high-speed countercurrent chromatography, and relates to a method used for preparing senkyunolide I and senkyunolide H from ligusticum wallichii extract via high-speed countercurrent chromatography and preparative liquid chromatography. According to the method, n-butyl alcohol, acetic acid, and water are mixed, and a layered mixture is obtained; an upper phase is collected, a high-speed countercurrent chromatographic column is filled with the upper phase, a host machine is rotated, a lower phase is pumped as a mobile phase, and the ligusticum wallichii extract is dissolved in the lower phase for sampling through a sampling valve; a SP-200 ultraviolet detector is used for detecting; and eluent containing senkyunolide I and senkyunolide H are collected. The method is simple in operation; preparation period is short; yield is high; senkyunolide I and senkyunolide H products with a total content more than 90% can be obtained; and continuous production can be realized.

Description

technical field [0001] The present invention relates to a kind of preparation method of senkyunolide I and H (senkyunolide I, senkyunolide H), particularly relate to a kind of utilizing high-speed counter-current chromatography to extract from Rhizoma Chuanxiong ( Ligusticum chuanxiong Hort.) Method for the preparation of chuanxionglide I and H in the extract. Background technique [0002] Chuanxiong ( Ligusticum chuanxiong Hort.) is a Umbelliferae plant whose medicinal parts are rhizomes. Chuanxiong is a traditional Chinese medicine in my country. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and laxative. Clinically, it is often used for blood deficiency and chlorosis, dizziness and palpitations, irregular menstruation, abdominal pain due to deficiency and cold, constipation due to dryness of the intestine, arthralgia due to rheumatism, traumatic injuries, etc. [0003] Phthalides are a class of com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/88
CPCC07D307/88
Inventor 王新平
Owner SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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