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Specific chromatogram construction method of compound composition containing ligusticum wallichii, borneol and artificial musk and pharmaceutical preparation

A technology of artificial musk and pharmaceutical preparations, applied in the field of analysis and detection, can solve the problems of short analysis time, cumbersome processing methods, lack of integrity and integrity of quality control, etc., and achieve the effect of rapid detection ability

Active Publication Date: 2022-07-29
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ligusticum chuanxiong, borneol, and artificial musk in the preparation are rich in volatile small molecular compounds, which can easily pass through the blood-brain barrier and play a positive role in the prevention and treatment of cardiovascular and cerebrovascular diseases. However, the volatile components of the compound preparation lack quality control methods, so there are It is necessary to test it, carry out quality control on Chuanxiong, borneol, and artificial musk, and improve its quality control system
[0003] Prior art CN112611823A quantitatively and qualitatively detects borneol and artificial musk. It adopts the method of preparing a portion of the test solution and performing gas chromatography analysis twice. Although the analysis time of this method is short, it lacks the detection of Rhizoma Chuanxiong. analysis to achieve the goal
The data of CN108226370A shows that a compound preparation is subjected to solid-phase extraction treatment, and some index components borneol, camphor and menthol are detected by gas phase. and integrity

Method used

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  • Specific chromatogram construction method of compound composition containing ligusticum wallichii, borneol and artificial musk and pharmaceutical preparation
  • Specific chromatogram construction method of compound composition containing ligusticum wallichii, borneol and artificial musk and pharmaceutical preparation
  • Specific chromatogram construction method of compound composition containing ligusticum wallichii, borneol and artificial musk and pharmaceutical preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Embodiment 1 Investigation of extraction solvent

[0087] Preparation of A reference substance solution: take appropriate amount of borneol, musk ketone, ligustilide, yangchuanxiong lactone A, yangchuanxiong lactone I, butenyl phthalide, accurately weighed, add petroleum ether to dissolve, respectively. Make a mixed reference substance solution containing 16μg, 2μg, 15μg, 20μg, 2μg, 2μg per 1ml, shake well, that is;

[0088] B take four parts of the test sample, accurately weigh 0.2g, put them in a stoppered conical flask, precisely add 10ml of acetone, petroleum ether, ethyl acetate, and petroleum ether, respectively, and ultrasonically (power 250W, frequency 40kHz) for 30 minutes, Let cool, shake well, filter, take the filtrate, the test results are as follows figure 1 ; figure 1 It is a graph of the results of the investigation of the extraction solvent in Example 1 of the present invention;

[0089] C Establishment: Precisely draw 1 μl each of the reference solutio...

Embodiment 2

[0096] Take three parts of the test sample, accurately weigh 0.2g, put them in a stoppered conical flask, accurately add 10ml of petroleum ether, ultrasonically (power 250W, frequency 40kHz) for 15 minutes, 30 minutes, 45 minutes, let cool, shake Evenly, filter, take the filtrate, the test results are as follows figure 2 , figure 2 It is a graph of the results of the investigation of the extraction time in Example 2 of the present invention; the rest are the same as in Example 1.

[0097] The results show that there is no significant difference in the peak resolution with different extraction times. In order to ensure that the target peak in the solution can be fully extracted, the extraction time of the test solution is selected as 30 minutes.

Embodiment 3

[0099] Take three parts of the test sample, accurately weigh 0.2g, put them in a stoppered conical flask, accurately add 5ml, 10ml, 20ml of petroleum ether, ultrasonically (power 250W, frequency 40kHz) for 30 minutes, let cool, shake well, filter , take the continuous filtrate, the test results are as follows image 3 ; image 3 It is the result figure of the investigation of solvent addition amount in Example 3 of the present invention; the rest are the same as in Example 1.

[0100] The results show that there is no significant difference in peak information and resolution for extraction with different solvent additions. In order to ensure sufficient extraction, the solvent addition of the test solution is 10ml.

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Abstract

The invention provides a specific chromatogram construction method of a compound composition containing ligusticum wallichii, borneol and artificial musk and a pharmaceutical preparation, which comprises the following steps: A) dissolving a raw material to be detected by using a solvent, and extracting to obtain a liquid to be detected; the method comprises the following steps: respectively taking one or more of borneol, musk ketone, ligustilide, senkyunolide A, senkyunolide I and butylidenephthalide as reference substances, and dissolving with a solvent to obtain a reference substance solution; and B) determining the to-be-detected solution and the reference substance solution by adopting a gas chromatographic method to obtain the GC characteristic chromatogram of the compound composition containing ligusticum wallichii, borneol and artificial musk and the pharmaceutical preparation. According to the method, a characteristic spectrum method is established for the composition containing volatile components of ligusticum wallichii, borneol and artificial musk for the first time, 25 common characteristic peaks are confirmed, the relative retention time of the composition is stipulated, and a contrast characteristic spectrum is established, so that whether the composition and a compound preparation contain ligusticum wallichii, borneol and artificial musk medicinal materials or not can be quickly and accurately identified; and high-efficiency, simple, convenient and rapid detection capability is realized.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a compound composition containing chuanxiong, borneol and artificial musk, and a method for constructing a characteristic map of a pharmaceutical preparation. Background technique [0002] A traditional Chinese medicine compound pharmaceutical preparation for preventing or treating senile dementia disclosed in the prior art, the prescription medicinal flavors include: Chuanxiong, ginseng, Panax notoginseng, leeches, borneol, artificial musk (CN106491680B), used for treating qi deficiency and blood weakness, phlegm blocking and clearing orifices , Severe memory loss caused by congestion and obstruction of collaterals, inconvenience, unsteady gait, apathy, lethargy or irritability, even not knowing family members, not knowing the way home and other symptoms, has a good effect in the treatment of Alzheimer's disease, can be Significantly improve learning, memory, cogn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/047Y02A50/30
Inventor 周厚成周靖惟黄美华胡昌江陈蓉张开元梅国荣张晨徐红应黄宇孙纪元
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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