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A method for determining the fingerprint of the extract of Radix Astragali and Chuanxiong

A technology of Chuanxiong extract and fingerprints, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve problems such as the impact on the quality of finished products

Active Publication Date: 2017-01-18
上海百洋制药股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the quality of preparations is interrelated with the quality of raw materials and intermediates. It is very important to control the quality of raw materials from the source, but intermediates are affected by the environment, equipment and conditions in the production process, which will also have a certain impact on the quality of finished products. influences

Method used

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  • A method for determining the fingerprint of the extract of Radix Astragali and Chuanxiong
  • A method for determining the fingerprint of the extract of Radix Astragali and Chuanxiong
  • A method for determining the fingerprint of the extract of Radix Astragali and Chuanxiong

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Experimental program
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Effect test

Embodiment 1

[0024] 1. Selection of detection wavelength

[0025] The established test wavelength is determined by the following experiments. Comparing the fingerprint spectra at 208nm, 254nm and 280nm, it is found that the baseline at 280nm is stable, the number of chromatographic peaks is large, and the absorption intensity is large. Finally, 280nm is selected as the detection wavelength.

[0026] 2. Selection of chromatographic column and optimization of mobile phase elution gradient program

[0027] ① Kromasil 100-5C18 column

[0028] Preparation of the test solution: Take about 0.7g of the extract of Astragalus and Chuanxiong, accurately weigh it, add 20ml of 70% ethanol to extract with ultrasonic (35KHz, 280W) for 30min, let it cool, make up the lost solvent weight, pass through a 0.22μm microporous filter Membrane, take the continued filtrate, that is.

[0029] Chromatographic conditions: chromatographic column is Kromasil 100-5C18 chromatographic column; column temperature is 30°...

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Abstract

The invention relates to a method for determining fingerprint chromatography of radix astragali and ligusticum wallichii extract products, which comprises the following steps: 1)taking Radix Astragali and Ligusticum wallichii extract product fine powder, preciously weighing, adding methanol, performing ultrasonic extraction, filtering and drying a filtrate, dissolving residue by ethanol and metering volume, filtering by a filter membrane, taking a subsequent filtrate to obtain a tested object solution; 2)taking a ferulic acid reference substance, a senkyunolide I reference substance, a calycosin glucoside reference substance, an ononin reference substance, a calycosin reference substance and a fermlononetin reference substance, preciously weighing, respectively adding methanol to prepare a reference substance solution; and 3)respectively and preciously absorbing the tested object solution and the reference substance solution, and injecting a high efficiency liquid chromatography for determining to obtain the fingerprint chromatography of radix astragali and ligusticum wallichii extract products. The method has active effect for guiding clinical medication and effective feeding guidance to the bulk drugs during the production process, and ensuring the reliable quality; the method has the advantages of convenient and fast operation, so that similarity result can be obtained, the quality of the traditional Chinese medicinal materials radix astragali and ligusticum wallichii extract products can be evaluated, and the result is objective and accurate.

Description

technical field [0001] The invention belongs to the field of fingerprints of traditional Chinese medicines, in particular to a method for determining the fingerprints of extracts of astragalus and chuanxiong. Background technique [0002] Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge. Chuanxiong is the dried rhizome of Ligusticum chuanxiong, an umbrella plant. Astragalus and Chuanxiong were respectively recorded in the Chinese Pharmacopoeia 2010 Edition. They are commonly used traditional Chinese medicines and appear frequently in compound Chinese medicines. Such as Fangji Huangqi Decoction, Dachuanxiong Pills, Qishe Pills, etc. [0003] Qishe Pill is a traditional Chinese medicine compound preparation researched and developed in recent years for the treatment of cervical spondylosis. The whole prescription is composed of six herbs including astragalus, chuanxiong, artificial musk, Qingfengteng, Fangji and artifi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张宁潘一峰
Owner 上海百洋制药股份有限公司
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