140 results about "Liquiritigenin" patented technology

The disclosure provides compositions comprising liquiritigenin, or derivatives, or prodrugs, useful as estrogen receptor beta selective agonists. The disclosure also provides methods of treating menopausal symptoms, and estrogen-dependent disorders, with said compositions.

The invention discloses coriobacterium sp. AUH-Julong21 and a use of coriobacterium sp. AUH-Julong21 in liquiritigenin conversion. A preservation number of coriobacterium sp. AUH-Julong21 is china general microbiological culture collection center (CGMCC) NO.5306. A preparation method of coriobacterium sp. AUH-Julong21 comprises the following steps of incubating a coriobacterium sp. AUH-Julong21 strain into a liquid medium for culture, wherein an inoculation rate is in a range of 10 to 15%, adding crude liquiritigenin into the liquid medium, carrying out culture in an anaerobic work station for 2 to 3 days, carrying out culture extraction twice by ethyl acetate, filtering the extract, drying the filtered extract by distillation, adding 100% of a methanol solution into the dried extract, and carrying out product separation preparation in a high performance liquid chromatograph (HPLC) by a preparation column. The coriobacterium sp. AUH-Julong21 can efficiently convert crude liquiritigenin into David viburnum aglycones, and when concentration is in a range of 0.4 to 1.6 millimoles per liter, a 1,1-dipheny-2-picrylhydrazyl (DPPH) extracorporeal clearance rate of the produced David viburnum aglycone is obviously higher than that of substrate liquiritigenin having p less than 0.01.

The disclosure provides compositions comprising liquiritigenin, or derivatives, or prodrugs, useful as estrogen receptor beta selective agonists. The disclosure also provides methods of treating menopausal symptoms, and estrogen-dependent disorders, with said compositions.

The invention relates to a quality detection method of a traditional Chinese medicine preparation. The method comprises the following steps: respectively taking gallic acid, albiflorin, paeoniflorin,ferulic acid, liquiritin, beta-ecdysterone, senkyunolide I, glycyrrhizin, cinnamic acid, cinnamyl aldehyde, paeonol, ammonium glycyrrhizinate and ligustilide as reference substances, and establishinga fingerprint spectrum of the traditional Chinese medicine preparation by adopting high performance liquid chromatography; identifying Chinese angelica, the rhizome of chuanxiong, radix paeoniae alba,moutan bark, ginseng, cassia bark, licorice root and the root of bidentate achyranthes of the traditional Chinese medicine preparation by adopting thin-layer chromatography. The traditional Chinese medicine preparation is prepared by using the following raw materials: Chinese angelica, the rhizome of chuanxiong, radix paeoniae alba, cassia bark, moutan bark, zedoray rhizome, ginseng, licorice root and the root of bidentate achyranthes. With the two detection ways, comprehensive quality evaluation can be conducted on the meridian-warming decoction traditional Chinese medicine preparation. Themethod is simple, convenient, high in accuracy and high in reproducibility; a scientific basis can be provided for quality detection and evaluation of the meridian-warming decoction traditional Chinese medicine preparation, and the product quality is effectively controlled.

The present invention discloses preparation process of licoflavone from licorice and licorice slag, and features licoflavone is extracted from crushed licorice or licorice slag after extracting glycyrrhetic acid through adding organic solvent for soaking, filtering, and concentrating the filtrate; and that the prepared licoflavone contains licochalcone, isoliquiritigenin, glabidin, liquiritin, liquiritigenin and other components. The preparation process is simple, high in yield and suitable for industrial production.

The invention provides a full-function facial mask. The facial mask comprises the following components in percentage by mass: 0.01-2 percent of hyaluronic acid, 0.01-1 percent of reduced glutathione, 0.01-2 percent of L-vitamin C, 0.01-2 percent of tranexamic acid, 0.01-2 percent of liquiritigenin, 0.01-5 percent of vitamin E, 0.01-1 percent of EGF lyophilized powder, 0.01-2 percent of active peptide, 0.01-3 percent of SOD, 0.01-2 percent of arbutin, 0.1-2 percent of allantoin, 0.5-5 percent of glycerinum, 0.5-5 percent of propylene glycol and the balance of water, wherein the effective components are independently embedded with liposome or coated with nano-microcapsules before being mixed. The activated effective components of the facial mask are independently coated or embedded with transporters, and are delivered to the corium layer or the superficial corium layer by virtue of transportation of the transporters to achieve the effects of quadruple whitening, triple repairing and double moistening.

A synergistic clean extraction method of effective components in licorice root adopts a solvent to synchronously extract the effective components in licorice root in order to obtain a total extract product, and the total extract product is separated one by one according to physical and chemical properties of all the components. The method comprises the following steps: 1, preparing the total extract product of the licorice root; 2, separating water-soluble and fat-soluble components; 3, separating glycyrrhizic acid from licorice pigments, licorice glucose and licorice polysaccharides in the water-soluble components; 4, separating the licorice pigments from the licorice glucose and the licorice polysaccharides; 5, separating the licorice glucose from the licorice polysaccharides; 6, separating and purifying glabridin in the fat-soluble components; 7, separating and purifying liquiritin and isoliquiritin in the fat-soluble components; 8, separating and purifying isoliquiritigenin and liquiritigenin in the fat-soluble components; 9, separating and purifying licochalcone A in the fat-soluble components; 10, separating and purifying licorice lignin in the fat-soluble components; and 11, separating and purifying licorice cellulose in the fat-soluble components.

A grosvenor momordica fruit tablet as a nutritive food for preventing and treating cough, asthma and constipation is prepared from grosvenor momordica fruit, lily bulb, liquorice root and haw through pretreating, extracting by decocting, and conventional tabletting. Its advantage is rich nutrients including saponin, glycyrrhizic acid, liquiritigenin, polyose, vitamines, minerals and trace elements.

The invention discloses a multi-flavone component extracting and purifying method from liquorice, which comprises the following steps: 1. extracting and condensing glycyrol liquid; 2. adsorbing through large-hole resin; eluting the water and alcohol gradient; 3. remaining eluent of different density alcohol to continue different technologies( freezing, adding concentrated sulfuric acid-carbinol to do reflux hydrolysis; adding 80-100 order polyamide column; eluting water and carbinol; passing Sephadex LH-20 column; eluting again to produce multi-flavone component within iquiritin, liquiritigenin, isoliquiritin and isoliquiritin glucose apiin).

The invention provides a composition for face whitening. The composition comprises the following components in percentage by mass: 0.01-1% of reduced glutathione, 0.01-2% of levo vitamin C, 0.01-3% of tranexamic acid, 0.01-3% of liquiritigenin and the balance being water, wherein before being mixed, active ingredients are independently embedded in liposomes or coated with nano microcapsules. The active and effective ingredients of the composition are independently coated or embedded through transfer carriers respectively and are conveyed to a corium layer or a shallow corium layer through the transfer carriers, so as to block synthesis of melanin, inhibit aggregation of the melanin, reduced formed spots and improve skin brightness.

The invention discloses a method used for separating and identifying flavonoid matters in tobacco by adopting liquid chromatography-mass spectrography. The method comprises the following steps: a sample is ground and dried, an extractant is added according to the standard that 0.1 ml of the extractant is added into 1 mg of the sample for extraction and centrifugation, a supernatant liquor is collected, water and chloroform are added according to the standard that 0.5 ml of the water and 1 ml of the chloroform are added into 1 ml of the supernatant liquor for centrifugation, and the supernatant liquor is collected for analysis; 153+ and 151- are adopted as characteristic ions for scanning, parent ions are extracted for second level mass spectrum scanning, and an obtained spectrogram is compared with a spectral library to determine a chemical structure and verified through a reference substance; the molecules and ions of the identified matters and myricetin, dihydromyricetin, catechinic acid / epicatechin, daidzein, glycitein and glycyrrhizin / isoliquiritigenin are adopted as characteristic ions for scanning, the molecules and ions of the searched glycosylation flavonoid matters are used as the parent ions for the second level mass spectrum scanning, an obtained spectrogram is compared with the spectral library to determine a chemical structure and verified through the reference substance. According to the invention, the method is suitable for the analyzing and detecting micro even trace flavonoid matters.

Disclosed herein is an extraction method for increasing liquiritigenin content in Glycyrrhizae Radix et Rhizoma or Glycyrrhizae radix extract, the method comprises: using water, an organic solvent, or a mixed solvent of water and the organic solvent to Glycyrrhizae Radix et Rhizoma to extract a product containing liquiritin (Step 1); and introducing water, an organic solvent, or a mixed solvent of water and the organic solvent into the product containing liquiritin extracted in Step 1, adding acid or base into the solution, and hydrolyzing the mixture to prepare a product containing liquiritigenin (Step 2).

The invention provides a method for measuring content of eighteen ingredients of Huangqi Jianzhong Wan (astragalus mongholicus pill for invigorating the stomach and regulating the middle warmer). Themethod includes the steps of subjecting the Huangqi Jianzhong Wan to ultrasonic extraction and centrifugation, extracting the liquid supernate, detecting the liquid supernate by an ultra performance liquid chromatography-tandem mass spectrometry positive and negative ion mode, preparing a mixed standard product solution from standard products of the eighteen ingredients, building a standard curve,and carrying out the methodological study. The method can realize measuring the eighteen ingredients of the Huangqi Jianzhong Wan, namely ononin, formononetin, calycosin, calycosin-7-glucoside, pterocarpan, astraisoflavan-7-O-beta-D-glucoside, isoflavane, isoliquiritin, liquiritigenin, liquiritin, glycyrrhetinic acid, cinnamic acid, isomucronulatol 7-O-beta-glucoside, astragaloside I, astragaloside II, astragaloside III, astragaloside IV and gallic acid. In the method, the sample pre-treatment process is simple and accordingly time for measurement is short; the method has the advantages of high specificity, high flexibility, good reproducibility and short analysis time, and can be applied to monitoring of production quality of the Huangqi Jianzhong Wan.

The present invention is compound interferon inducing agent lozenge, relates to biomedicine engineering, and aims at providing lozenge capable of inducing body to generate endogenous interferon and with high curative effect, less side effect, small dosage and wide application. Each of the compound interferon inducing agent lozenge contains attenuated Newcastle disease virus vaccine or deactivated Newcastle disease virus vaccine 10-120 hemagglutination units, astragalus root 1-2000 mg, liquiritigenin 1-1000 mg, supplementary material and stabilizer. The compound interferon inducing agent lozenge is suitable for preventing SARS and other viral infectious respiratory tract diseases, and has also auxiliary treatment effect on various kinds of tumor, hepatitis B and hepatitis C.

The invention discloses novel medical application of isopentenyl isoflavone compounds in licorice roots. Through systematic research on the chemical components and activity screening of the licorice roots, it is discovered that the isopentenyl isoflavone compounds including licoricidin, angustone A and glyasperin D of which the structures are similar have the significant activity on inhibition of cancer cell proliferation and inhibition of protein tyrosine phosphatase 1B (PTP1B), licoricidin and glyasperin D have the significant activity on inhibition of acetylcholine esterase (AChE), and glyasperin D also has the significant activity on inhibition of tyrosinase. On the basis of the discovery, the active compounds or salt, ester and solvent compounds, stereoisomers, tautomer and prodrugs which are acceptable in pharmacy of the active compounds and mixtures of the compounds can be used for preparing anti-cancer drugs, PTP1B inhibitors, antidiabetic drugs, tyrosinase inhibitors, freckle removing and whitening products, AChE inhibitors, anticholinesterase drugs and the like.

The invention relates to a preparation method of a composite probiotic ice cream. The preparation method comprises the following steps of (1) weighing raw materials according to the formula: whole milk powder, soybean milk powder, water, cane sugar, beta-cyclodextrin, soybean protein powder, inulin, honeysuckle flower powder, Chinese wolfberry fruit powder and liquiritigenin for standby application; and (2) uniformly mixing and stirring the weighed raw materials in step (1), then performing homogenizing, performing sterilization and performing ageing to obtain an ice cream mixture, then adding0.003-0.008 part of composite probiotics to the ice cream mixture, performing uniform stirring to obtain a mixture, sending the mixture to a condensing and freezing machine for condensing and freezing, after condensing and freezing, performing filling, and performing hardening, so as to obtain the probiotic ice cream. The ice cream made by the preparation method is rich in nutrition, various probiotics and traditional Chinese medicine components, and has good health-care functions. According to the method, the ice cream is compounded from specific components in specific parts by weight, so that the survival rate of probiotics for the probiotic ice cream in the preparation and preservation process can be increased, and normal exertion of the health-care functions of the probiotic ice creamcan be guaranteed.

The present invention is compound interferon inducing agent lozenge, relates to biomedicine engineering, and aims at providing lozenge capable of inducing body to generate endogenous interferon and with high curative effect, less side effect, small dosage and wide application. Each of the compound interferon inducing agent lozenge contains attenuated Newcastle disease virus vaccine or deactivated Newcastle disease virus vaccine 10-120 hemagglutination units, astragalus root 1-2000 mg, liquiritigenin 1-1000 mg, supplementary material and stabilizer. The compound interferon inducing agent lozenge is suitable for preventing SARS and other viral infectious respiratory tract diseases, and has also auxiliary treatment effect on various kinds of tumor, hepatitis B and hepatitis C.

The invention discloses an oil pipeline detergent and a preparation method thereof, belonging to the technical field of industrial detergents. The oil pipeline detergent comprises the following components in parts by weight: 2-5 parts of gallnuts, 1.5-3 parts of small cyrtomium fortunei, 1-2.5 parts of sodium glycyrrhizinate, 3-6 parts of pomegranate barks, 2-4 parts of sodium tripolyphosphate, 0.1-1 part of sodium metasilicate pentahydrate, 10-13 parts of sodium diethylhexyl sulfosuccinate, 4-12 parts of iso-tridecanol polyoxyethylene ether, 1-3 parts of urotropin, 0.5-5 parts of nonionic fluorocarbon surfactant, 2-5 parts of fatty alcohol polyoxyethylene ether, 1-2 parts of scale inhibitor and 120-150 parts of water. The preparation method is simple; the prepared oil pipeline detergent can be used for rapidly and thoroughly removing oil dirt, wax and the like on an oil pipeline; in addition, the raw materials of the oil pipeline detergent contain no strong acid and alkaline substances, and water is used as a solvent, so that the oil pipeline detergent is free of corrosion to crude oil equipment, is free of injury to human bodies, is safe and environment-friendly and is easy to popularize and apply.

The hair restoring shampoo is scurf eliminating shampoo added with ginger juice medicine, which is prepared through mixing and grinding ginseng, angelica, dahurian angelica root, arborvidae leaf, pinellia tuber and other ten kinds of Chinese medicinal materials, soaking the medicine powder in ginger juice and filtering. The hair restoring shampoo contains also AES, needle shaped K12, betaine CAB-35, emulsified silicone oil, pearly luster slurry, active liquiritigenin, sodium chloride, pigment, preservative, essence and deionized water. The hair restoring shampoo has obvious hair restoring effect, scurf eliminating effect and other advantages.

The invention discloses the use of isoliquiritigenin as a medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors, which belongs to technical field ofmedicaments. The invention discloses the use of isoliquiritigenin as a medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors. Researches show thatthe surgical resection of primary tumors can promote tumor metastasis. At present, no ideal therapeutic method or medicament for treating malignant tumor metastasis and relapse after the resection ofthe primary tumors is available. Experiments implemented by the inventor prove that the isoliquiritigenin has a remarkable inhibition effect on the relapse and metastasis of various malignant tumors after the surgical resection of the primary tumors. Based on the effect, the isoliquiritigenin can be used in clinic prevention or treatment of the relapse and metastasis of lung cancer, liver cancer,breast cancer, colorectal cancer and other various malignant tumors after the surgical resection of the primary tumors and has the obvious advantages of high safety, low price, good patient complianceand the like. The effect of the isoliquiritigenin may be related to the inhibition of the isoliquiritigenin on the self renewal and multiplication capacity of cancer stem cells.

The technical field of the invention relates to preparation of a drug preparation method with liquiritigenin and combination containing liquiritigenin which is used to treat senile dementia and Parkinson disease. The liquiritigenin is added with excipient and processed into agents such as capsules, tablets, granules and injection, etc. The liquiritigenin has protection and nutrition functions to neurons and has no obvious female activity, thereby being capable of improving memory ability of dementia mouse and adlibitum sports ability of Parkinson disease model mouse.

The invention discloses an endophytic bacterial strain Penicillium commune CGMC CNO.6113 and an application in the flavone production. Compared with coordinative bacterial strains, the Penicillium commune CGMC CNO.6113 bacterial strain is apparent in the flavone production in mycology. When the bacterial strain is fermented, main ingredients of glycyrrhiza flavonoids of liquiritin, glycyrrhizin, isoliquiritigenin and the like can be produced, and the bacterial strain can be applied in antioxidation, senescence delaying, inflammation elimination and the like as a prevention and treatment method, accordingly, the endophytic fungi for producing flavones and the application thereof provide a novel approach for the development and application of glycyrrhiza flavonoids and have wide applicationvalues.

The invention relates to anticancer liquiritigenin oral preparation and injection preparation, and a preparation method thereof, belonging to a medical technology field. The oral preparation comprises a particulate agent, tablets, capsules, pills and softgel, and the injection preparation comprises small hydro-acupuncture, freeze-dried powder injection, glucose injection and sodium chloride injection. The liquiritigenin oral preparation contains 0.1-100g liquiritigenin per 1000 preparation unit, and the injection preparation contains 0.1-100g liquiritigenin per 1000mL. Pharmaceutic adjuvants contain a diluent, a wetting agent, a disintegrating agent, a flow aid, a cosolvent, an excipient and an osmotic pressure regulator. The liquiritigenin preparation provided by the invention is safe and reliable, and has functions for preventing and treating cancers. And the anticancer medicament is used for preventing and treating lung cancer, liver cancer, stomach cancer, colorectal carcinoma, leukemia, etc., and is a new and effective anticancer medicine.

A method for measuring contents of 14 chemical components in a traditional Chinese medicine composition is disclosed. HPLC-MS / MS is adopted to simultaneously measure the contents of the 14 chemical components in the composition. The composition is a pulse-invigorating heart-tonifying pill. The 14 chemical components include emodin, lobetyolin, 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside, glycyrrhizic acid, liquiritin, liquiritigenin, polydatin, rutoside, isoliquiritigenin, ononin, verbascoside, gallic acid, schizandrin, and rhein. The method includes building standard curves of the 14chemical components; acquiring multi-reaction ion chromatograms of the 14 chemical components in the pill; and determining the contents of the 14 chemical components in the pill. The method has characteristics of high sensitivity, high reliability, a high efficiency and capability of being rapid. Through measuring the contents of the 14 chemical components in the pill, quality control on the pillcan be more effectively performed, thus ensuring clinical treatment effects of the pill.

The invention discloses finely cut tobacco leaves with flavonoid compound essence for cigarettes and a method for preparing the finely cut tobacco leaves. The method includes weighing adhesive with the weight of 0.1%-1% of the weight of the flavonoid compound essence; dissolving the adhesive in water and then adding the dissolved adhesive into the flavonoid compound essence; uniformly stirring the dissolved adhesive and the flavonoid compound essence to obtain soft materials; extruding the soft materials by the aid of an extruder to obtain thin and long strips; rounding the strips by the aid of a rounder; drying the strips to obtain the finely cut tobacco leaves with the flavonoid compound essence. The flavonoid compound essence comprises, by weight, 20%-50% of liquiritigenin, 20%-50% of eugenol, 10%-40% of luteolin and 5%-20% of naringin. The finely cut tobacco leaves with the flavonoid compound essence and the method have the advantages that the finely cut tobacco leaves have obvious merits in the aspect of reducing phenol in smoke, the phenol reduction rate can reach 15-45%, and the free radical reduction rate is 8-25%; the flavonoid compound essence has excellent natural smell and can be added to a cigarette holder, so that the cigarettes can have unique and strong extra aroma.

The invention discloses a method for extracting liquiritigenin from liquorice. The method comprises the following steps of: (1) crushing the liquorice, extracting the powder by using 1 mol / L hydrochloric acid solution, filtering, regulating the filtrate to be neutral by using a 1 mol / L sodium hydroxide solution, and obtaining a crude liquiritigenin extract; and (2) injecting the crude liquiritigenin extract into a mixed resin column, and performing elution, separation, purification and re-crystallization. The liquiritigenin extracted by the method is a natural extract, and is high in purity, low in cost, high in production efficiency and stable and controllable in quality.

The invention relates to a method for controlling quality of a liquorice medicinal material and a liquorice preparation. The method concretely relates to the step of simultaneously determining the content of the liquorice medicinal material and the content of six main active ingredients, namely liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid and glycyrrhetinic acid in the liquorice preparation by utilizing a high performance liquid chromatography. A new method for controlling the quality of the liquorice and the liquorice preparation is established and the six main active ingredients, namely liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid and glycyrrhetinic acid are quantitatively determined simultaneously by utilizing the high performance liquid chromatography. Confirmed by methodological investigation and test, the method is simple and convenient, correct in result and good in reproducibility, the defect of the existing quality control is overcome, and the quality control on the liquorice and the liquorice preparation is perfect and scientific.

The invention provides a full-function facial mask. The facial mask comprises the following components in percentage by mass: 0.01-2 percent of hyaluronic acid, 0.01-1 percent of reduced glutathione, 0.01-2 percent of L-vitamin C, 0.01-2 percent of tranexamic acid, 0.01-2 percent of liquiritigenin, 0.01-5 percent of vitamin E, 0.01-1 percent of EGF lyophilized powder, 0.01-2 percent of active peptide, 0.01-3 percent of SOD, 0.01-2 percent of arbutin, 0.1-2 percent of allantoin, 0.5-5 percent of glycerinum, 0.5-5 percent of propylene glycol and the balance of water, wherein the effective components are independently embedded with liposome or coated with nano-microcapsules before being mixed. The activated effective components of the facial mask are independently coated or embedded with transporters, and are delivered to the corium layer or the superficial corium layer by virtue of transportation of the transporters to achieve the effects of quadruple whitening, triple repairing and double moistening.

The invention discloses a microbial fermentation method for preparing licorice root fermentation liquor with high safety performance and whitening and anti-aging effects and a product. The method comprises the steps of inoculating an unfermented licorice root solution with activated yeast strains, and conducting fermentation for 35-45 h at the temperature of 25-35 DEG C, the pH value of 6.5-9.0 and the rotating speed of 80-160 r / min, so as to obtain the licorice root fermentation liquor. Researches find that by the adoption of the method, the content of glabridin and licochalcone A in the licorice root fermentation liquor can be reduced remarkably, and the content of the active ingredients isoliquiritigenin and isoliquiritoside which have MMPs inhibition and collagen stimulation effects is increased remarkably. Therefore, the licorice root fermentation liquor prepared with the method has broad application prospects in preparation of cosmetic products, especially cosmetic products with high safety performance and whitening and anti-aging effects.

The invention provides a process for increasing liquiritigenin content, which takes liquorice as a raw material and is fermented by saccharomycetes. The process comprises the following steps: taking liquorice medicinal materials, sterilizing, adding cellulase, water and saccharomycetes, and fermenting. Experimental results show that the liquiritigenin content in the licorice root fermented productobtained by the fermentation process is remarkably increased, and the liquiritigenin content is increased by more than 30 times after liquorice medicinal materials with the same mass are fermented bythe specific process disclosed by the invention. The fermentation process disclosed by the invention is low in cost, simple to operate and easy for large-scale production, and has a wide applicationprospect in preparation of liquorice products with high liquiritigenin content.