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Method for extracting multiple liquorice flavone form liquorice

A technology of licorice and licorice glycosides is applied in the new field of extracting and purifying various licorice flavonoids from licorice, which can solve the problems of low yield, long production cycle, environmental pollution and the like, and achieve the effects of reducing production costs and saving organic solvents.

Inactive Publication Date: 2006-11-22
XIAMEN BERSI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The patent document whose publication number is 1477104 in the prior art 文献1 and "Research on the Chemical Constituents of Yellow Glycyrrhizae" (Acta Pharmaceutica Sinica, 1989, 24(7): 525-531) 文献2 and "Chemical Constituents of Yellow Glycyrrhizae (II)" (Acta Botanica, 1991, 33(4): 314-322) 文献3 Two articles introduced the methods of extracting and purifying various flavonoids, but their methods all used toxic and harmful organic solvents such as chloroform, dichloromethane, etc., which will pollute the environment on the one hand, and on the other hand, extract Residues in the extract will also limit the application of
The method of document 1 has used expensive special-purpose ultrafiltration equipment and membrane material again, and the method technological process of document 2, 3 is complicated, and production cycle is long, and yield is low, needs to consume a large amount of organic solvents, makes their production cost relatively high

Method used

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  • Method for extracting multiple liquorice flavone form liquorice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment one (referring to accompanying drawing " process flow diagram "):

[0025] Take 1kg of Ural licorice and grind it into 10000mL of 70% ethanol for ultrasonic extraction at room temperature twice, each time for 30min, coarsely filter with 200 mesh nylon filter cloth, centrifuge at 5,000rmp for 10min, and concentrate the centrifuged supernatant under reduced pressure (0.05MPa, 50℃ ), evaporate ethanol to 1 / 10 volume, take the concentrated supernatant and adsorb on a macroporous resin column (filled with cross-linked polystyrene resin), and successively elute with water and 10-30% ethanol gradient, and the elution flow rate is 1.5BV / h. Use HPLC or TLC to detect the effluent, and wash until the main substance cannot be detected in the effluent. Take 30% ethanol eluate and refrigerate at 4°C to precipitate 14g of liquiritin.

Embodiment 2

[0026] Embodiment two (referring to accompanying drawing " process flow diagram "):

[0027] Take 1 g of liquiritin obtained in the previous example, put it into 50 ml of aqueous solution containing 5% concentrated sulfuric acid and 40% methanol for reflux hydrolysis for 1.5 hours, concentrate under reduced pressure (0.05MPa, 50°C), and distill off the methanol to 1 / 10 volume , passed through a Sephadex LH-20 column, eluted with water and 10-50% methanol under normal pressure in sequence, and used HPLC or TLC to detect the effluent, and washed until the main substance could not be detected in the effluent. The 50% methanol eluate was refrigerated at 4°C, and 0.25 g of white needle crystals of liquiritigenin was precipitated.

Embodiment 3

[0028] Embodiment three (referring to accompanying drawing " process flow diagram "):

[0029] Take 1 kg of Ural licorice and grind it into 10,000 mL of 80% ethanol for ultrasonic extraction at room temperature twice, each time for 30 min, filter with a 200-mesh nylon filter cloth, centrifuge at 5,000 rpm for 10 minutes, and concentrate the centrifuged supernatant under reduced pressure (0.05 MPa, 50 °C ), evaporate ethanol to 1 / 10 volume, take the concentrated supernatant and absorb it on a macroporous resin column, and then elute with water and 10-50% ethanol gradient successively, get 50% ethanol eluate, concentrate and evaporate ethanol, the concentrated solution Put on a polyamide column of 80-100 mesh, eluted with water and 5-60% methanol at normal pressure gradient successively, take 60% methanol eluate and concentrate under reduced pressure, pass through Sephadex LH-20 column, and elute with 40-70% methanol at normal pressure gradient Elution starts from the upper colu...

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Abstract

The invention discloses a multi-flavone component extracting and purifying method from liquorice, which comprises the following steps: 1. extracting and condensing glycyrol liquid; 2. adsorbing through large-hole resin; eluting the water and alcohol gradient; 3. remaining eluent of different density alcohol to continue different technologies( freezing, adding concentrated sulfuric acid-carbinol to do reflux hydrolysis; adding 80-100 order polyamide column; eluting water and carbinol; passing Sephadex LH-20 column; eluting again to produce multi-flavone component within iquiritin, liquiritigenin, isoliquiritin and isoliquiritin glucose apiin).

Description

technical field [0001] The invention relates to a method for extracting and purifying pharmacologically active components from Chinese medicinal materials, especially a new method for extracting and purifying various flavonoid components such as liquiritin, liquiritigenin, isoliquiritin, isoliquiritigenin, glucoapigenin and the like from licorice . Background technique [0002] Licorice is called "harmonizing Chinese medicine" and "harmonizing medicine" by many Chinese medical books, and it is used in almost all Chinese medicine prescriptions to coordinate and harmonize other Chinese medicinal materials to play a role. In order to bring Chinese medicine to the world, the research on various Chinese medicinal materials including licorice is becoming more and more in-depth and detailed. Different from the traditional practice of using the whole herb as medicine, the current trend is to study the active ingredients contained in various Chinese medicinal materials and these The...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07C45/79C07C49/835C07H1/08
Inventor 傅博强王小如
Owner XIAMEN BERSI BIOLOGICAL TECH
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