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Microbial fermentation method for preparing licorice root fermentation liquor with high safety performance and whitening and anti-aging effects and product

A technology of microbial fermentation and high safety, which is applied to the microbial fermentation method of licorice fermentation liquid and its products, the fermentation method of licorice and its products, and can solve the problems of potential safety hazards and reduction

Active Publication Date: 2017-08-11
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The compounds isolated from licorice so far are glycyrrhizin, glycyrrhetinic acid, glycyrrhizin, glabridin, isoliquiritin, neoliquiritin, neoisoliquiritin, glycyrrhizin, isoliquiritigenin and glycyrrhizidine, licorice Alcohol, isoglycyrrhizol, 7-methylcoumarin, umbelliferone and other dozens of compounds, glabridin (Glabridin) is an internationally recognized licorice whitening active ingredient, but the present invention found that glabridin It is toxic to melanocytes and fibroblasts, and can stimulate the expression of mmps, suggesting that glabridin as a whitening ingredient has certain safety hazards to the human body. Other whitening active ingredients with high safety become an urgent need

Method used

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  • Microbial fermentation method for preparing licorice root fermentation liquor with high safety performance and whitening and anti-aging effects and product
  • Microbial fermentation method for preparing licorice root fermentation liquor with high safety performance and whitening and anti-aging effects and product
  • Microbial fermentation method for preparing licorice root fermentation liquor with high safety performance and whitening and anti-aging effects and product

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 The research of licorice monomer to the inhibitory effect of tyrosinase

[0030] In order to determine the tyrosinase inhibitory effect of each licorice monomer in the licorice fermentation broth, the present invention selected the following 6 main licorice monomers to carry out the tyrosinase inhibition test, and measured the IC50 values ​​of the 6 main licorice monomers at the same time , 6 main monomers are: Liquiritigenin, Isoliquiritigenin, Isoliquiritin, Glabridin, Glycyrrhizic Acid and Licochalcone A.

[0031] The result is as figure 1 shown, from figure 1 It can be seen from the results that the inhibition rate of 6 main monomers to tyrosinase is as follows: glabridin > glycyrrhizic acid > isoliquiritigenin > isoliquiritin > licochalcone A > liquiritigenin.

Embodiment 2

[0032] The cytotoxicity of embodiment 2 licorice monomer

[0033] In order to determine the cytotoxicity of each licorice monomer in the licorice fermentation broth, the present invention tested the cytotoxicity of six licorice monomers to fibroblasts by the WST-1 method, and the test results are shown in Table 1.

[0034] Table 1 Toxicity of different concentrations of licorice active monomers (μM) to fibroblasts

[0035]

[0036] From the results in Table 1, it can be seen that isoliquiritigenin and isoliquiritin showed low toxicity in the toxicity evaluation test; glabridin and licochalcone A showed high toxicity in the toxicity evaluation test, and there are certain safety hazards to the human body .

Embodiment 3

[0037] The influence of embodiment 3 licorice monomer on the expression of COLLA1, MMP1mRNA

[0038] 1. Test method

[0039]Real-time fluorescent quantitative PCR (qRT-PCR) analysis was used to measure the changes in the mRNA quantity of type 1 collagen and the collagenase representative gene that decomposes type 1 collagen to determine the changes of type 1 collagen and collagenase. Split human skin fibroblast (nHDF) cells into 3x10 cells in 60mm culture plate 5 1 / well, cultured for 24 hours. Then, a certain concentration of sample solution was added and cultured for 24 hours. After the cultured cells were harvested, 1 ml TRIzol reagent (Life Technology) was added to lyse the cells and extract their total mRNA. use The concentration and purity of the total mRNA were checked by an ultra-micro spectrophotometer (wherein only the total mRNA with a purity of more than 2.0 was selected). Using reverse transcriptase (M-MLV reverse transcriptase), the total mRNA was reverse tr...

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Abstract

The invention discloses a microbial fermentation method for preparing licorice root fermentation liquor with high safety performance and whitening and anti-aging effects and a product. The method comprises the steps of inoculating an unfermented licorice root solution with activated yeast strains, and conducting fermentation for 35-45 h at the temperature of 25-35 DEG C, the pH value of 6.5-9.0 and the rotating speed of 80-160 r / min, so as to obtain the licorice root fermentation liquor. Researches find that by the adoption of the method, the content of glabridin and licochalcone A in the licorice root fermentation liquor can be reduced remarkably, and the content of the active ingredients isoliquiritigenin and isoliquiritoside which have MMPs inhibition and collagen stimulation effects is increased remarkably. Therefore, the licorice root fermentation liquor prepared with the method has broad application prospects in preparation of cosmetic products, especially cosmetic products with high safety performance and whitening and anti-aging effects.

Description

technical field [0001] The invention relates to a fermentation method of licorice and a product thereof, in particular to a microbial fermentation method and a product thereof for preparing licorice fermentation liquid with high safety, whitening and anti-aging effects, and belongs to the technical field of cosmetics. Background technique [0002] Licorice (Glycyrrhiza uralensis Fisch), known as the "whitening queen", is widely used in cosmetics and has a huge market demand. It can not only inhibit tyrosinase, scavenge free radicals, and inhibit the release of histamine, so as to achieve whitening and anti-aging , anti-inflammatory effect, and at the same time can improve rough skin, water shortage and other problems. Licorice whitening has been reported in many foreign whitening cosmetics patents, and licorice extract is often added directly. Licorice extract is contained in many cosmetics that claim to have whitening effects. Because licorice extract is generally dark in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/60A61K8/25A61Q19/02A61Q19/08
CPCA61K8/25A61K8/602A61K8/97A61K2800/85A61Q19/02A61Q19/08
Inventor 董银卯李丽张小慧刘有停
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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