142 results about "Glabridin" patented technology

The invention relates to a method for synthesizing glabridin. The method comprises the following steps: using acetophenone protected by phenol hydroxy as a raw material, carrying out a Willgerodt-Kindler reaction to obtain aryl phenylacetic acid, and carrying out a Friedel-Crafts reaction to obtain an isoflavones compound; causing the isoflavones compound to carry out Pd / C catalytic hydrogenation to obtain a isoflavanone compound; and causing the isoflavanone compound to carrying out a crclizationreaction, a conyl reduction reaction and a removing phenolic hydroxyl group protection group reaction to obtain the glabridin. The operation and the serparation of the steps of the method are simple, the yield is higher, the used reagents are common reagents, are cheap and are easily obtained, the path is shorter, and the tptal yield is not lower than 20 percent.

The invention belongs to the technical field of cosmetics and particularly a biological skin brightening facial mask liquid and a preparation method thereof. The biological skin brightening facial mask liquid is prepared from the following components in percentage by mass: 0.1-2 percent of glabridin, 0.5-3 percent of microalgae skin brightening agent, 0.5-2.5 percent of citrus peel extract, 0.05-2percent of alpha-arbutin, 0.1-3 percent of radix paeoniae extract, 0.5-5 percent of yeast extract and the balance of matrix. The prepared facial mask liquid is capable of reducing melanin from multiple aspects and in multiple ways aiming to various parts in the process from generation to discharge of the melanin, has the remarkable effects of skin whitening and brightening, is capable of replenishing water, preserving moisture, resisting ageing and keeping skin healthy at the same time and does not have irritation.

The invention discloses a high-efficiency separation and purification method of a glabridin monomer. The method comprises the steps of: extracting raw material dried roots of Glycyrrhiza Glycyrrhiza glabra L which is a leguminous plant with an organic reagent A; and then extracting with an organic reagent B; carrying out chromatography by using polyamide columns and macroporous adsorbent resin columns; and finally separating and purifying a target area solution by using high effective liquid chromatogram columns; carrying out on-line test by using HPLC (High Performance Liquid Chromatography), and purposely collecting a glabridin monomer solution to obtain the glabridin monomer with the concentration higher than 98%. The invention has the advantages of high production quantity, high yield rate and good production quality, and is suitable for industrial production.

A synergistic clean extraction method of effective components in licorice root adopts a solvent to synchronously extract the effective components in licorice root in order to obtain a total extract product, and the total extract product is separated one by one according to physical and chemical properties of all the components. The method comprises the following steps: 1, preparing the total extract product of the licorice root; 2, separating water-soluble and fat-soluble components; 3, separating glycyrrhizic acid from licorice pigments, licorice glucose and licorice polysaccharides in the water-soluble components; 4, separating the licorice pigments from the licorice glucose and the licorice polysaccharides; 5, separating the licorice glucose from the licorice polysaccharides; 6, separating and purifying glabridin in the fat-soluble components; 7, separating and purifying liquiritin and isoliquiritin in the fat-soluble components; 8, separating and purifying isoliquiritigenin and liquiritigenin in the fat-soluble components; 9, separating and purifying licochalcone A in the fat-soluble components; 10, separating and purifying licorice lignin in the fat-soluble components; and 11, separating and purifying licorice cellulose in the fat-soluble components.

The invention discloses a method for producing high-purity glabridin. The method comprises the following steps: drying and crushing glycyrrhiza glabra, decocting the glycyrrhiza glabra with alkaline water, and filtering and concentrating the decoction to obtain alkaline water decoction; loading the decoction onto a resin chromatographic column, and eluting the resin chromatographic column with water and ethanol in turn; collecting 45 to 55 percent ethanol eluent, and concentrating the eluent to obtain glycyrrhizic acid; collecting 85 to 95 percent ethanol eluent, concentrating the eluent, loading the concentrated solution onto a polyamide chromatographic column, and eluting the polyamide chromatographic column with the ethanol of different percentage concentrations by volume in turn; collecting 70 to 75 percent ethanol eluent, and concentrating the eluent to be dry so as to obtain the glabridin; and refluxing and dissolving the glabridin into acetone, and cooling, crystallizing, filtering and drying mixture to obtain a finished product. According to the method, the alkaline water is used to replace an organic solvent for extraction, a macroporous resin column is used to replace a silica chromatographic column for separation, and the ethanol solution is used to replace the mixed eluent such as chloroform and the like, so the cost is lower, the safety of the product is better, and the purity and the yield of the product are higher.

The present invention relates to the production process of extracting active component glabridin from common licorice as a kind of medicinal plant. The production process includes the following steps: drying and crushing common licorice, organic solvent reflux extraction or diacolation extraction, concentrating the extract liquid and decompresson drying to obtain coarse product; dissolving the coarse product in chloroform, gradient elution with mixed chloroform-acetone solvent in silica gel column, silica gel thin layer chromatography until dilute sulfuric acid development, collecting target solution, decompression concentration, vacuum drying, dissolving in ethanol, filtering, decompression concentrating the filtrate and vacuum drying to obtain the product. The product is yellow brown powder and has active component content up to 44.64 %.

The invention relates to a method for asymmetrically synthesizing glabridin with optical purity under catalysis of a ruthenium compound. The method comprises the following steps: 1) taking isoflavoneprotected by a protection group as a raw material and carrying out dynamic kinetic asymmetric hydrogen transfer reaction under the catalysis effect of a ruthenium trichloride compound and the action of an acid-alkali buffering system to obtain chiral isoflavol with an absolute configuration being (3R, 4R); 2) removing hydroxyl of the chiral isoflavol under the action of triethylsilane and trifluoroacetic acid to obtain a product with an absolute configuration being (R); 3) removing a protection group of the product with the configuration being (R) in step 2) under an acidic or alkaline condition to obtain the glabridin with the configuration (R) and the optical purity. The method provided by the invention can be used for synthesizing the glabridin with the optical purity in a high-yield and high-stereoselectivity manner; the obtained product is completely the same as that of the glabridin extracted from glycyrrhiza glabra and can be used for replacing the glabridin derived from naturalplants to be industrially applied.

The invention discloses a method for extracting high pure glabridin from glycyrrhiza glabra. The method comprises the following steps of (1) adding a mixed solution in dry powder of glycyrrhiza glabra; extracting for 10-30 min under ultrasonic frequency of 30-80 Hz and ultrasonic power of 80-200 W at a temperature of 10-50 DEG C, wherein the mixed solution is prepared by ethyl acetate, acetone and absolute ethyl alcohol; (2) suction filtering, The method is simple in operational technology, short in process flow and low in cost. The mixed solution of ethyl acetate, acetone and absolute ethyl alcohol is used as an extraction solution, and a mixed resin is used for extraction and purification, so that the purity of the obtained glabridin is high.

The invention discloses a synthesis method of glabridin. According to the method, cheap easy-to-obtain 7-hydroxycoumarin is used as a raw material to react with 2-methyl-3-butyne-1-alcohol for building alkyne ester; then, bromine atoms are introduced into alpha sites of obtained alpha-beta-unsaturated compounds; through seven-step reaction including rearrangement, Szuki coupling as core reactionsfor key intermediate (IV) building, dihydroxy compound obtaining through conjugated double bond reduction, Mitsunobu ether formation and methyl removal, glabridin is fast and efficiently synthesized at the total yield of 20 percent finally. Compared with the prior art, the synthesis method has the following advantages that the raw materials are cheap and can be easily obtained; the total route isshort; the total yield is high; the glabridin can be fast and efficiently synthesized.

The invention relates to a method for producing glabridin from glycyrrhiza residue, mainly comprising the following processes of: concentrating the glycyrrhiza residue into syrup after aqueous extraction; carrying out activated carbon adsorption after dissolving in methanol; washing by using hot methanol; adsorbing methanol washing solution by macroporous adsorption resin AB-8; washing by using water and alcohol respectively; collecting and concentrating alcohol washing solution; recrystallizing by using organic solvent with 3-5 times amount; and obtaining the glabridin after drying. The method uses the methanol for dissolving syrupy extracting solution, the activated carbon adsorption method elution can be avoided, and the phenomenon of mixed solvent does not occur, thus avoiding the situations in the existing process for extracting and preparing the glabridin of long synthetic step, high cost, a large number of used organic solvents and not being beneficial to environmental protection, and also effectively simplifying procedures and reducing the production cost; especially by using the residue of the glycyrrhizic acid for extraction, the wastes are changed into valuables, the time is saved and the product content is high. The method can also be applied to extract glabridin directly from glycyrrhiza.

The invention belongs to the technical field of separation and purification, and particularly relates to a method for extracting and purifying glabridin from glycyrrhiza glabra residues. The method comprises the following steps: (1) extraction: sequentially adding the glycyrrhiza glabra residues and an extraction solvent into an extraction tank, performing reflux extraction twice, and distilling off the solvent under reduced pressure, so as to obtain a concentrate for later use; (2) separation: sequentially adding silica gel and petroleum ether into a conical flask for column packing, performing sample loading on the concentrate obtained in Step (1), adding an elution solvent for elution, performing TLC (Thin Layer Chromatography) detection to collect a glabridin component, and distilling off the solvent under reduced pressure at 30 to 70 DEG C, so as to obtain an orange oily matter; (3) crystallization: adding a crystallization solvent into the oily matter obtained in Step (2), performing stirring and suction filtration, and flushing and drying a filter cake to obtain the glabridin. The method is high in extraction efficiency, the operation steps are convenient and efficient, and the production cost is greatly reduced.

The invention relates to glabridin freeze-dried liposome and a preparation method thereof. The glabridin freeze-dried liposome comprises, by weight, 65-70 parts of soybean phospholipid, 30-35 parts of cholesterol, 0.5-3.0 parts of glabridin, 1000-1500 parts of anhydrous ethanol and 70-120 parts of a phosphate buffer. The preparation method comprises the following steps of 1, weighing soybean phospholipid and cholesterol, putting the soybean phospholipid and cholesterol in an eggplant-shaped bottle, and dissolving the soybean phospholipid and cholesterol in anhydrous ethanol, 2, carrying out decompression on the eggplant-shaped bottle to evaporate the solvent, 3, preparing a glabridin solution, 4, preparing a glabridin phosphate solution, 5, preparing a glabridin freeze-dried liposome suspension, 6, preparing glabridin freeze-dried liposome by a freeze-drying method, and 7, carrying out vacuum-pumping by a freeze-drier for 5-10min so that water is completely evaporated and the glabridin freeze-dried liposome is obtained. The glabridin freeze-dried liposome has a reasonable design, has very strong external transdermal absorption performances and provides experiment basis for a bio-pharmaceutical industry.

The invention relates to a glabridin nanosuspension and a preparation method thereof. The formulation of the glabridin nanosuspension comprises the following ingredients by weight percentage: 0.5-20% of glabridin, 0.1-20% of a stabilizer and the balance of water. The glabridin nanosuspension is prepared by adopting ultrasonic pre-dispersion in combination with a high-pressure homogenization method. According to the glabridin nanosuspension disclosed by the invention, the solubility of the glabridin is greatly improved, the service efficiency is improved, the dosage of the glabridin in cosmetics is decreased, and the production cost is reduced.

The invention discloses a method for separating an active component-glabridin contained in natural product glycyrrhiza glabra. The method comprises the following steps of: preparing SILMs (Supported Ionic Liquid Membranes) under the pressurizing condition of 0.1-0.3 MPa by selecting ionic liquid; dissolving a crude glabridin product into an ethanol water solution to prepare a glabridin raw material solution; adopting an alkaline water solution as a back extraction solution; fixing the SILMs between a raw material solution pool and a back extraction solution pool, respectively placing two magnetic stirrers at the outer sides of the raw material solution pool and the back extraction solution pool, carrying out the extraction and back extraction of the glabridin under the condition of magnetic stirring, wherein the extraction rate of the glabridin is 70%-90%, and the total recovery rate is 70%-85%. The method disclosed by the invention carries out the separation and purification of the glabridin by adopting a supported ionic liquid membrane technology and realizes the high selectivity, greenness and environmental friendliness of the glabridin.

The invention relates to a safety type essence formula with whitening effect, and a process thereof. The safety type essence formula mainly comprises the following raw materials in percentage by weight: 0.05 to 0.08 percent of gellan gum, 0.1 to 0.2 percent of xanthan gum, 5 to 8 percent of glycerinum, 10 to 12 percent of propylene glycol, 0.1 to 0.2 percent of sodium hyaluronate, 0.2 to 0.3 percent of hydrolyzed hyaluronic acid, 0.1 to 0.2 percent of jojoba wax PEG-120 ester, 1 to 2 percent of nicotinamide, 0.3 to 0.4 percent of a viscosity modifier, 0.5 to 1 percent of a snail secretion filtrate extract, 0.5 to 1 percent of a lucid ganoderma extract, 0.005 to 0.01 percent of glabridin, 0.1 to 0.2 percent of EGF, 0.5 to 1 percent of whitening compound, 1 to 2 percent of a yeast extract, 0.5 to 1 percent of an aspergillus fermentation product extract and 1 to 2 percent of trimethyl pentaphenyl trisiloxane. The safety type essence formula achieves the whitening effect while guaranteeing safety.

The invention relates to a glabridin molecularly imprinted polymer, as well as a preparation method and application thereof. The preparation method comprises the following steps of: taking glabridin as a template, adding the template, functional monomers, a cross-linking agent, an initiator and a solvent into a reaction kettle according to the mass ratio of (1-3): (2-8): (15-30): (0.05-0.2): (20-50), mixing, performing ultrasonic degassing, then charging nitrogen, removing oxygen, sealing, and then polymerizing for 10-24h in a constant-temperature water bath at the temperature of 40-60 DEG C to obtain the glabridin molecularly imprinted polymer with template molecules; and grinding, eluting and drying the obtained polymer to obtain the molecularly imprinted polymer for purifying the glabridin. The obtained glabridin molecularly imprinted polymer can be used as column chromatography packing for column packing so as to be applied in extraction of the glabridin from glycyrrhiza glabra, and the glabridin molecularly imprinted polymer has the advantages of strong selectivity, high adsorption capacity and high purity of the obtained glabridin.

The invention relates to a preparation method for a high-purity glabridin monomer, and relates to the preparation method for the high-purity glabridin monomer by separation from licorice. The preparation method mainly solves the problems of complicated preparation process, low separation efficiency, long separation time, and large consumption amount of a solvent. The method provided by the invention is characterized by comprising the following steps: preparation of a glabridin extract; concentration of glabridin by using a reversed-phase C18 column chromatography: successively eluting the extract with alcohols respectively with a concentration of 50%, 70% and 95%, respectively collecting eluents, and subjecting the collected eluent of the alcohol with the concentration of 70% to vacuum concentration until the eluent is dried so as to obtain crude glabridin; and separation of the above-mentioned crude glabridin with a high-speed countercurrent chromatographic technology: placing a separating solvent in a separatory funnel and carrying out complete shaking, then carrying out standing and layering, dissolving the above-mentioned crude glabridin in the separating solvent, and respectively measuring the concentrations of supernatant and subnatant solutions containing the glabridin with a highly-efficient liquid-phase chromatographic method. The preparation method for the high-purity glabridin monomer provided by the invention has short separation time and high recovery rate, is free of pollution, and saves the solvent.

The invention provides a culture method of glycyrrhiza hairy roots and a method for preparing glycyrrhiza total flavonoids from glycyrrhiza hairy roots and culture solution thereof. The method comprises the following steps: culturing glycyrrhiza hairy roots in a fluid nutrient medium which does not contain Tween to the growth peak period; culturing the obtained glycyrrhiza hairy roots in the growth peak period in a fluid nutrient medium which contains Tween for more than 5 days; and eluting by ethanol with different concentration through a polyamide chromatography column, and separating the total flavonoids from the culture solution, wherein the transfer rate of the glycyrrhiza total flavonoids is 97.23%, the transfer rate of glycyrrhiza chalcone A is 98.14%, and the transfer rate of Glabridin is 97.54%. The invention also provides the glycyrrhiza total flavonoids prepared by the method and medical applications of the glycyrrhiza total flavonoids.

The invention discloses a whitening composition and the application thereof in whitening cosmetics. The whitening composition contains the following components in weight percentage: 1-10% of lipid soluble VC derivatives, 0.05-0.2% of Glabridin, 1-5% of nicotinamide, 1-5% of hydrogenated soya lecithin, 0.1-0.5% of a gorgonia extracting solution and 0.5-2% of an amino-acid humectant. According to the whitening composition prepared by the invention, the whitening and speckle removing effects are obvious, the whitening composition can be quickly adsorbed by skin, the irritation to the skin is weak and the obvious moisture-holding, anti-inflammatory and antiallergic effects and effective skin brightening effect are realized.

The invention provides a method of extracting high-purity glabridin from licorice waste residue by adoption of a new technology. The licorice waste residue is adopted as a raw material. A finished product of the glabridin is obtained by processes of extraction, impurity removal and adsorption. The method is advantageous in that: the high-purity glabridin is extracted from the licorice waste residue so that the waste resource is reutilized and the cost of the glabridin is largely reduced; a mixed solvent is used for extraction so that the extraction rate of the glabridin is maximized and the output is largely increased; room-temperature extraction is adopted, and therefore the production process is easy to operate, extracted impurities are less, the purity of the glabridin is high, solvent volatility is reduced, safety in production is improved and the cost is reduced; compared with purification processes adopting silica gel columns or a plurality of solvents for repeated extraction in the prior art, problems of low output and low production are solved and purification processes are largely simplified, thus facilitating industrial popularization and production; and the yield of the product is largely increased.

The invention belongs to the technical field of separation and purification and provides a glabridin preparation method, glabridin prepared according to the glabridin preparation method and cosmetics.The glabridin preparation method includes: performing alcohol extraction on glycyrrhiza glabra residues obtained by aqueous extraction to obtain alcohol extract liquid, and concentrating to obtain alcohol extract; then, sequentially adopting macroporous resin and polyamide for column chromatography separation to obtain eluent containing glabridin, and recrystallizing to obtain glabridin. By alcohol extraction of glycyrrhiza glabra residues subjected to aqueous extraction, more of water-soluble impurities in glycyrrhiza glabra are removed before alcohol extraction, high-purity glabridin is separated out through twice specific column chromatography separation, high-purity glabridin is finally obtained through recrystallization, and consumption of environment-unfriendly solvents in a preparation process is avoided. High-purity glabridin is prepared through alcohol extraction, twice column chromatography and recrystallization, and simple process route, low cost and suitableness for industrial production are realized.

The invention discloses spot-removing cream. The spot-removing cream is composed by the following raw materials: ceto-stearyl alcohol, dimethyl silicone oil, white mineral oil, caprylic / capric triglyceride, echium lycopsis oil, triclosan, vitamin E, water, glycerin, xanthan gum, an amino acid moisturizing agent, sodium hyaluronate, an anchoic acid derivative, paeonol, glabridin, propylene glycol and supguard GM-BP. In the formula of the spot-removing cream, the anchoic acid derivative and paeonol act collectively, thereby eliminating tingling caused by adopting the anchoic acid derivative individually, broadening blood capillary of skins, promoting blood circulation and enhancing defense capability of the skins; in combination with glabridin and vitamin E, activities of tyrosinase, hallachrome tautomerase can be inhibited, hyperpigmentation can be alleviated, and spot-restraining and skin whitening effects are enhanced; and the existence of the amino acid moisturizing agent and echium lycopsis oil allows the spot-removing cream to have the functions of moisturizing and resisting inflammation and anaphylaxis.

The invention relates to a whitening essence formula which is mainly prepared from the following raw materials in percentage by weight: 79.19% to 86.045% of deionized water, 0.1% to 0.2% of carbomer, 0.1% to 0.2% of transparent xanthan gum, 2% to 3% of glycerol, 6% to 10% of propylene glycol, 0.1% to 0.2% of allantoin, 0.1% to 0.2% of sodium hyaluronate, 0.1% to 0.2% of hydrolyzed hyaluronic acid, 0.3% to 0.5% of antioxidant, 0.1% to 0.2% of PH regulator, 0.005% to 0.01% of glabridin, 0.05% to 0.1% of EGF and 5% to 6% of skin conditioning agent. The whitening essence formula can be used to achieve a whitening effect, is safe and has no side effects.

The invention relates to a glabridin microcapsule including 0.8-3.0 parts of glabridin, 0.8-2.0 parts of ethyl cellulose, 0-0.3 part of polyethylene glycol and 0.2-2.5 parts of sodium carboxymethyl starch. The invention discloses a preparation method. The glabridin microcapsule prepared by the invention has good stability, good encapsulation effect, good fluidity, good dissolution, yield up to above 90% and drug loading higher than 50%; and the microcapsule has a quasi-circular shape and particle size between 60-80 mesh, so as to achieve the purposes of slow release and controlled release.

The invention relates to a composition for whitening and brightening skin and an application of the composition for whitening and brightening skin to cosmetics. The composition comprises the followingcomponents of a glabridin carrier, hydroxyethylpiperazine ethane sulfonic acid (HEPES), an endothelin antagonist and plant composite essence. The composition for whitening and brightening skin can effectively restrain tyrosinase activity, restrain melanocyte disintegration, eliminate free radicals, soften cutin, improve skin withstanding capacity and the like; a glabridin carrier technique and plant fresh and living ingredients coexist, powerful efficacy of whitening skin and lightening freckles of the endothelin antagonist is used for cooperation, in the presence of the HEPES, synergistic interaction is achieved, and excellent efficacy of whitening skin and brightening skin can be achieved.

The invention discloses a natural handmade soap with whitening and freckle removal functions and a preparation method thereof. The natural handmade soap comprises the following components in percentage by weight: 0.2-0.6% of glabridin, 0.2-0.5% of dragon's blood, 0.2-0.8% of safflower alcohol extract, 0.3-0.6% of lavender essential oil, 0.8-4% of natural vegetable fat, 5-25% of glycerol, 0.1-0.5% of hyaluronic acid and 70-90% of sodium soap. The glabridin in the formula has favorable whitening and freckle removal effects; and the dragon's blood and safflower can enhance the vascular permeability, promote local blood circulation and metabolism and enhance the effects of the glabridin. The natural handmade soap can be directly used for skin cleaning, and has the effects of whitening and moisturization. The invention also discloses a preparation method of the natural handmade soap. The natural handmade soap is prepared from natural plant extracted components, is inirritative to skin, and is an effective cleaning and care product which can be used for a long time.

The invention discloses a glabridin composition and a preparation method thereof. The glabridin composition comprises glabridin, undecylenoyl phenylalanine, nicotinamide, ascorbyl palmitate phosphatetrisodium, and alpha-lipoic acid. The glabridin composition reduces the production of dopachrome by inhibiting the activity of tyrosinase during the process of forming dopaquinone from tyrosine, blocks the melanin transfer pathway, and accelerates the exfoliation of stratum corneum and metabolism, so as to achieve beauty effects such as whitening and freckle removing.

The invention discloses a repairing and whitening composition and a preparation method thereof. The repairing and whitening composition contains acetyl chitosamine, glabridin, hydrogenated lecithin, butyl methoxydibenzoylmethane, tocopherol, piperine, dipotassium glycyrrhizinate, potassium methoxysalicylate, tranexamic acid, alpha-arbutin, nicotinamide, a camellia extract and 3-o-ethyl ascorbic acid. According to the whitening action mechanism, the whitening effect is enhanced in an oriented multi-target multi-way mode, and butyl methoxydibenzoylmethane resists ultraviolet damage; dipotassiumglycyrrhizinate and acetyl chitosamine have the effects of reducing inflammation and ultraviolet stimulation; arbutin and glabridin inhibit tyrosinase activity; tocopherol inhibits tyrosine from beingoxidized into dopa; 3-o-ethyl ascorbic acid reduces dopaquinone into dopa; glabridin inhibits the activity of dopa pigment tautomerase and the activity of DHICA oxidase; tranexamic acid and nicotinamide inhibit melanin transport; nicotinamide and potassium-4-methoxysalicylate promote epidermis renewal; and eight target points act together and coordinate with each other to achieve the whitening effect.

The invention discloses a whitening cream and a preparation method thereof. The whitening cream includes whitening ingredients, a penetration enhancer and a substrate, wherein the whitening ingredients include chamomile extract, glabridin, potassium 4-methoxysalicylate, nicotinamide and magnesium ascorbyl phosphate; the penetration enhancer includes hydrogenated lecithin, borneol and di-diethoxy diethylene glycol cyclohexane 1,4-dicarboxylate. The whitening cream can effectively change the barrier function of the stratum corneum of the skin, thereby promoting the transdermal absorption of nutrients, the whitening ingredients are reasonably matched, the production and transfer of melanin are inhibited, and the metabolism is accelerated to improve the whitening effect.