142 results about "Glabridin" patented technology

The invention belongs to the technical field of cosmetics and particularly a biological skin brightening facial mask liquid and a preparation method thereof. The biological skin brightening facial mask liquid is prepared from the following components in percentage by mass: 0.1-2 percent of glabridin, 0.5-3 percent of microalgae skin brightening agent, 0.5-2.5 percent of citrus peel extract, 0.05-2percent of alpha-arbutin, 0.1-3 percent of radix paeoniae extract, 0.5-5 percent of yeast extract and the balance of matrix. The prepared facial mask liquid is capable of reducing melanin from multiple aspects and in multiple ways aiming to various parts in the process from generation to discharge of the melanin, has the remarkable effects of skin whitening and brightening, is capable of replenishing water, preserving moisture, resisting ageing and keeping skin healthy at the same time and does not have irritation.

The invention discloses a method for producing high-purity glabridin. The method comprises the following steps: drying and crushing glycyrrhiza glabra, decocting the glycyrrhiza glabra with alkaline water, and filtering and concentrating the decoction to obtain alkaline water decoction; loading the decoction onto a resin chromatographic column, and eluting the resin chromatographic column with water and ethanol in turn; collecting 45 to 55 percent ethanol eluent, and concentrating the eluent to obtain glycyrrhizic acid; collecting 85 to 95 percent ethanol eluent, concentrating the eluent, loading the concentrated solution onto a polyamide chromatographic column, and eluting the polyamide chromatographic column with the ethanol of different percentage concentrations by volume in turn; collecting 70 to 75 percent ethanol eluent, and concentrating the eluent to be dry so as to obtain the glabridin; and refluxing and dissolving the glabridin into acetone, and cooling, crystallizing, filtering and drying mixture to obtain a finished product. According to the method, the alkaline water is used to replace an organic solvent for extraction, a macroporous resin column is used to replace a silica chromatographic column for separation, and the ethanol solution is used to replace the mixed eluent such as chloroform and the like, so the cost is lower, the safety of the product is better, and the purity and the yield of the product are higher.

The invention relates to a method for producing glabridin from glycyrrhiza residue, mainly comprising the following processes of: concentrating the glycyrrhiza residue into syrup after aqueous extraction; carrying out activated carbon adsorption after dissolving in methanol; washing by using hot methanol; adsorbing methanol washing solution by macroporous adsorption resin AB-8; washing by using water and alcohol respectively; collecting and concentrating alcohol washing solution; recrystallizing by using organic solvent with 3-5 times amount; and obtaining the glabridin after drying. The method uses the methanol for dissolving syrupy extracting solution, the activated carbon adsorption method elution can be avoided, and the phenomenon of mixed solvent does not occur, thus avoiding the situations in the existing process for extracting and preparing the glabridin of long synthetic step, high cost, a large number of used organic solvents and not being beneficial to environmental protection, and also effectively simplifying procedures and reducing the production cost; especially by using the residue of the glycyrrhizic acid for extraction, the wastes are changed into valuables, the time is saved and the product content is high. The method can also be applied to extract glabridin directly from glycyrrhiza.

The invention belongs to the technical field of separation and purification, and particularly relates to a method for extracting and purifying glabridin from glycyrrhiza glabra residues. The method comprises the following steps: (1) extraction: sequentially adding the glycyrrhiza glabra residues and an extraction solvent into an extraction tank, performing reflux extraction twice, and distilling off the solvent under reduced pressure, so as to obtain a concentrate for later use; (2) separation: sequentially adding silica gel and petroleum ether into a conical flask for column packing, performing sample loading on the concentrate obtained in Step (1), adding an elution solvent for elution, performing TLC (Thin Layer Chromatography) detection to collect a glabridin component, and distilling off the solvent under reduced pressure at 30 to 70 DEG C, so as to obtain an orange oily matter; (3) crystallization: adding a crystallization solvent into the oily matter obtained in Step (2), performing stirring and suction filtration, and flushing and drying a filter cake to obtain the glabridin. The method is high in extraction efficiency, the operation steps are convenient and efficient, and the production cost is greatly reduced.

The invention relates to glabridin freeze-dried liposome and a preparation method thereof. The glabridin freeze-dried liposome comprises, by weight, 65-70 parts of soybean phospholipid, 30-35 parts of cholesterol, 0.5-3.0 parts of glabridin, 1000-1500 parts of anhydrous ethanol and 70-120 parts of a phosphate buffer. The preparation method comprises the following steps of 1, weighing soybean phospholipid and cholesterol, putting the soybean phospholipid and cholesterol in an eggplant-shaped bottle, and dissolving the soybean phospholipid and cholesterol in anhydrous ethanol, 2, carrying out decompression on the eggplant-shaped bottle to evaporate the solvent, 3, preparing a glabridin solution, 4, preparing a glabridin phosphate solution, 5, preparing a glabridin freeze-dried liposome suspension, 6, preparing glabridin freeze-dried liposome by a freeze-drying method, and 7, carrying out vacuum-pumping by a freeze-drier for 5-10min so that water is completely evaporated and the glabridin freeze-dried liposome is obtained. The glabridin freeze-dried liposome has a reasonable design, has very strong external transdermal absorption performances and provides experiment basis for a bio-pharmaceutical industry.

The invention relates to a glabridin nanosuspension and a preparation method thereof. The formulation of the glabridin nanosuspension comprises the following ingredients by weight percentage: 0.5-20% of glabridin, 0.1-20% of a stabilizer and the balance of water. The glabridin nanosuspension is prepared by adopting ultrasonic pre-dispersion in combination with a high-pressure homogenization method. According to the glabridin nanosuspension disclosed by the invention, the solubility of the glabridin is greatly improved, the service efficiency is improved, the dosage of the glabridin in cosmetics is decreased, and the production cost is reduced.

The invention discloses a method for separating an active component-glabridin contained in natural product glycyrrhiza glabra. The method comprises the following steps of: preparing SILMs (Supported Ionic Liquid Membranes) under the pressurizing condition of 0.1-0.3 MPa by selecting ionic liquid; dissolving a crude glabridin product into an ethanol water solution to prepare a glabridin raw material solution; adopting an alkaline water solution as a back extraction solution; fixing the SILMs between a raw material solution pool and a back extraction solution pool, respectively placing two magnetic stirrers at the outer sides of the raw material solution pool and the back extraction solution pool, carrying out the extraction and back extraction of the glabridin under the condition of magnetic stirring, wherein the extraction rate of the glabridin is 70%-90%, and the total recovery rate is 70%-85%. The method disclosed by the invention carries out the separation and purification of the glabridin by adopting a supported ionic liquid membrane technology and realizes the high selectivity, greenness and environmental friendliness of the glabridin.

The invention relates to a preparation method for a high-purity glabridin monomer, and relates to the preparation method for the high-purity glabridin monomer by separation from licorice. The preparation method mainly solves the problems of complicated preparation process, low separation efficiency, long separation time, and large consumption amount of a solvent. The method provided by the invention is characterized by comprising the following steps: preparation of a glabridin extract; concentration of glabridin by using a reversed-phase C18 column chromatography: successively eluting the extract with alcohols respectively with a concentration of 50%, 70% and 95%, respectively collecting eluents, and subjecting the collected eluent of the alcohol with the concentration of 70% to vacuum concentration until the eluent is dried so as to obtain crude glabridin; and separation of the above-mentioned crude glabridin with a high-speed countercurrent chromatographic technology: placing a separating solvent in a separatory funnel and carrying out complete shaking, then carrying out standing and layering, dissolving the above-mentioned crude glabridin in the separating solvent, and respectively measuring the concentrations of supernatant and subnatant solutions containing the glabridin with a highly-efficient liquid-phase chromatographic method. The preparation method for the high-purity glabridin monomer provided by the invention has short separation time and high recovery rate, is free of pollution, and saves the solvent.

The invention discloses a whitening composition and the application thereof in whitening cosmetics. The whitening composition contains the following components in weight percentage: 1-10% of lipid soluble VC derivatives, 0.05-0.2% of Glabridin, 1-5% of nicotinamide, 1-5% of hydrogenated soya lecithin, 0.1-0.5% of a gorgonia extracting solution and 0.5-2% of an amino-acid humectant. According to the whitening composition prepared by the invention, the whitening and speckle removing effects are obvious, the whitening composition can be quickly adsorbed by skin, the irritation to the skin is weak and the obvious moisture-holding, anti-inflammatory and antiallergic effects and effective skin brightening effect are realized.

The invention provides a method of extracting high-purity glabridin from licorice waste residue by adoption of a new technology. The licorice waste residue is adopted as a raw material. A finished product of the glabridin is obtained by processes of extraction, impurity removal and adsorption. The method is advantageous in that: the high-purity glabridin is extracted from the licorice waste residue so that the waste resource is reutilized and the cost of the glabridin is largely reduced; a mixed solvent is used for extraction so that the extraction rate of the glabridin is maximized and the output is largely increased; room-temperature extraction is adopted, and therefore the production process is easy to operate, extracted impurities are less, the purity of the glabridin is high, solvent volatility is reduced, safety in production is improved and the cost is reduced; compared with purification processes adopting silica gel columns or a plurality of solvents for repeated extraction in the prior art, problems of low output and low production are solved and purification processes are largely simplified, thus facilitating industrial popularization and production; and the yield of the product is largely increased.

The invention relates to a method by utilizing vanillin-sulfuric acid colorimetry to measure glabridin content. Collection process of chromogenic reagent absorbency value comprises the steps that: vanillin methanol solution with concentration of 20mg/mL is prepared, sulfuric acid methanol solution with concentration in percentage by volume of 30 percent is prepared, glabridin standard solution with concentration of 0.5mg/mL is prepared, glabridin standard test solution with range of the concentration of 0.01 to 0.5mg/mL is prepared, the absorbency value is collected, the chromogenic reagent is stable and reliable within 60 minutes under the room temperature, so the collection of the absorbency value information of the chromogenic reagent is guaranteed, and finally a linear regression equation is established. Being proved by a recovery test and a repeatability test: the method is stable to analyze, the repeatability conforms to the requirement, the method is proved to be completely reliable, the linear regression equation can provide a quantitative standard to the measurement of the content of the glabridin to be tested, and a test result and test precision, which are identical to the high-efficient liquid-phase spectrum, can be obtained.

The invention discloses spot-removing cream. The spot-removing cream is composed by the following raw materials: ceto-stearyl alcohol, dimethyl silicone oil, white mineral oil, caprylic/capric triglyceride, echium lycopsis oil, triclosan, vitamin E, water, glycerin, xanthan gum, an amino acid moisturizing agent, sodium hyaluronate, an anchoic acid derivative, paeonol, glabridin, propylene glycol and supguard GM-BP. In the formula of the spot-removing cream, the anchoic acid derivative and paeonol act collectively, thereby eliminating tingling caused by adopting the anchoic acid derivative individually, broadening blood capillary of skins, promoting blood circulation and enhancing defense capability of the skins; in combination with glabridin and vitamin E, activities of tyrosinase, hallachrome tautomerase can be inhibited, hyperpigmentation can be alleviated, and spot-restraining and skin whitening effects are enhanced; and the existence of the amino acid moisturizing agent and echium lycopsis oil allows the spot-removing cream to have the functions of moisturizing and resisting inflammation and anaphylaxis.

The invention relates to a whitening essence formula which is mainly prepared from the following raw materials in percentage by weight: 79.19% to 86.045% of deionized water, 0.1% to 0.2% of carbomer, 0.1% to 0.2% of transparent xanthan gum, 2% to 3% of glycerol, 6% to 10% of propylene glycol, 0.1% to 0.2% of allantoin, 0.1% to 0.2% of sodium hyaluronate, 0.1% to 0.2% of hydrolyzed hyaluronic acid, 0.3% to 0.5% of antioxidant, 0.1% to 0.2% of PH regulator, 0.005% to 0.01% of glabridin, 0.05% to 0.1% of EGF and 5% to 6% of skin conditioning agent. The whitening essence formula can be used to achieve a whitening effect, is safe and has no side effects.

The invention relates to a glabridin microcapsule including 0.8-3.0 parts of glabridin, 0.8-2.0 parts of ethyl cellulose, 0-0.3 part of polyethylene glycol and 0.2-2.5 parts of sodium carboxymethyl starch. The invention discloses a preparation method. The glabridin microcapsule prepared by the invention has good stability, good encapsulation effect, good fluidity, good dissolution, yield up to above 90% and drug loading higher than 50%; and the microcapsule has a quasi-circular shape and particle size between 60-80 mesh, so as to achieve the purposes of slow release and controlled release.

The invention relates to a composition for whitening and brightening skin and an application of the composition for whitening and brightening skin to cosmetics. The composition comprises the followingcomponents of a glabridin carrier, hydroxyethylpiperazine ethane sulfonic acid (HEPES), an endothelin antagonist and plant composite essence. The composition for whitening and brightening skin can effectively restrain tyrosinase activity, restrain melanocyte disintegration, eliminate free radicals, soften cutin, improve skin withstanding capacity and the like; a glabridin carrier technique and plant fresh and living ingredients coexist, powerful efficacy of whitening skin and lightening freckles of the endothelin antagonist is used for cooperation, in the presence of the HEPES, synergistic interaction is achieved, and excellent efficacy of whitening skin and brightening skin can be achieved.

The invention discloses a natural handmade soap with whitening and freckle removal functions and a preparation method thereof. The natural handmade soap comprises the following components in percentage by weight: 0.2-0.6% of glabridin, 0.2-0.5% of dragon's blood, 0.2-0.8% of safflower alcohol extract, 0.3-0.6% of lavender essential oil, 0.8-4% of natural vegetable fat, 5-25% of glycerol, 0.1-0.5% of hyaluronic acid and 70-90% of sodium soap. The glabridin in the formula has favorable whitening and freckle removal effects; and the dragon's blood and safflower can enhance the vascular permeability, promote local blood circulation and metabolism and enhance the effects of the glabridin. The natural handmade soap can be directly used for skin cleaning, and has the effects of whitening and moisturization. The invention also discloses a preparation method of the natural handmade soap. The natural handmade soap is prepared from natural plant extracted components, is inirritative to skin, and is an effective cleaning and care product which can be used for a long time.

The invention discloses a glabridin composition and a preparation method thereof. The glabridin composition comprises glabridin, undecylenoyl phenylalanine, nicotinamide, ascorbyl palmitate phosphatetrisodium, and alpha-lipoic acid. The glabridin composition reduces the production of dopachrome by inhibiting the activity of tyrosinase during the process of forming dopaquinone from tyrosine, blocks the melanin transfer pathway, and accelerates the exfoliation of stratum corneum and metabolism, so as to achieve beauty effects such as whitening and freckle removing.

The invention discloses a whitening cream and a preparation method thereof. The whitening cream includes whitening ingredients, a penetration enhancer and a substrate, wherein the whitening ingredients include chamomile extract, glabridin, potassium 4-methoxysalicylate, nicotinamide and magnesium ascorbyl phosphate; the penetration enhancer includes hydrogenated lecithin, borneol and di-diethoxy diethylene glycol cyclohexane 1,4-dicarboxylate. The whitening cream can effectively change the barrier function of the stratum corneum of the skin, thereby promoting the transdermal absorption of nutrients, the whitening ingredients are reasonably matched, the production and transfer of melanin are inhibited, and the metabolism is accelerated to improve the whitening effect.