Method by utilize vanillin-sulfuric acid colorimetry to measure glabridin content

Abstract

The invention relates to a method by utilizing vanillin-sulfuric acid colorimetry to measure glabridin content. Collection process of chromogenic reagent absorbency value comprises the steps that: vanillin methanol solution with concentration of 20mg/mL is prepared, sulfuric acid methanol solution with concentration in percentage by volume of 30 percent is prepared, glabridin standard solution with concentration of 0.5mg/mL is prepared, glabridin standard test solution with range of the concentration of 0.01 to 0.5mg/mL is prepared, the absorbency value is collected, the chromogenic reagent is stable and reliable within 60 minutes under the room temperature, so the collection of the absorbency value information of the chromogenic reagent is guaranteed, and finally a linear regression equation is established. Being proved by a recovery test and a repeatability test: the method is stable to analyze, the repeatability conforms to the requirement, the method is proved to be completely reliable, the linear regression equation can provide a quantitative standard to the measurement of the content of the glabridin to be tested, and a test result and test precision, which are identical to the high-efficient liquid-phase spectrum, can be obtained.

Description

technical field [0001] The invention belongs to the technical field of plant content determination, and in particular relates to a method for measuring glabridin content by a vanillin-sulfuric acid colorimetric method. Background technique [0002] Glycyrrhiza glabra ( Glycyrrhiza glabra L. ) The isoflavanes contained in it are referred to as glabridin ( Glabridin ), isoflavanes have attracted much attention due to their various pharmaceutical activities. The molecular formula of glabridin is C 20 h 20 o 4 , the molecular weight is 324.37. [0003] Some data show that glabridin can be used as an additive in whitening and freckle cosmetics, by inhibiting the activity of tyrosinase and dopachrome intermutation enzyme TRP-2, hindering the polymerization of 5, 6-dihydroxyindole DHI to prevent The formation of melanin, so as to achieve the effect of whitening the skin. [0004] There are also data showing that glabridin has the following properties: [0005] (1) It has...

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Problems solved by technology

[0008] Although the colorimetric method is currently a more commonly used quantitative analysis method for determining the content of substance

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