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Method for separating active component-glabridin contained in natural product glycyrrhiza glabra

A technology of ionic liquid and glabridin, which is applied in the direction of organic chemistry, can solve the problems of difficult regeneration and large consumption of ionic liquid, and achieve the effect of low cost, simple method and simple process

Inactive Publication Date: 2014-07-16
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our research group used imidazole ionic liquids to study the extraction of glabridin, and found that the extraction effect was good, but the ionic liquids were used directly for extraction, and there were problems such as large consumption of ionic liquids and difficult regeneration.

Method used

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  • Method for separating active component-glabridin contained in natural product glycyrrhiza glabra
  • Method for separating active component-glabridin contained in natural product glycyrrhiza glabra

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Using ionic liquid [Hmim][NTf 2 ] as the extraction agent, and the hydrophobic polyvinylidene fluoride membrane (PVDF) with a pore size of 0.22 μm as the support membrane material, pressurized to 0.2 MPa to prepare the ionic liquid support liquid membrane.

[0028] (2) Prepare a glabridin raw material solution with a concentration of 0.02-0.45 mg / mL with ethanol aqueous solution.

[0029] (3) Prepare a NaOH solution with a concentration of 0.1 mol / L with distilled water.

[0030] (4) The glabridin raw material solution obtained in step (1) and the 0.1mol / L NaOH stripping liquid obtained in step (2) are added respectively in the raw material liquid pool A and the stripping liquid pool B, and the magnetic stirring speed is 260rpm , the extraction time is 20 hours.

[0031] (5) The samples were determined by reverse-phase high-performance liquid chromatography (RP-HPLC). The extraction rate of glabridin was 70.13%-85.65%, and the total recovery rate of glabridin was ...

Embodiment 2

[0033] (1) Using ionic liquid [Hmim][NTf 2 ] As the extraction agent, the hydrophobic polyvinylidene fluoride membrane (PVDF) with a pore size of 0.22 μm was used as the support membrane material, and the pressure was increased to 0.2 MPa to prepare the ionic liquid support liquid membrane.

[0034] (2) Prepare a 0.25 mg / mL glabridin raw material solution with ethanol aqueous solution.

[0035] (3) Prepare a NaOH solution with a concentration of 0.02-0.5 mol / L with distilled water.

[0036] (4) step (1) obtains glabridin raw material liquid and the NaOH back extraction liquid that step (2) obtains and adds respectively in raw material liquid pool A, back extraction liquid pool B, and the rotating speed of magnetic stirrer is 260rpm, and extraction time is 20 hours.

[0037] (5) Determination of samples by reverse-phase high-performance liquid chromatography (RP-HPLC), the extraction rate of glabridin was 75.67%-86.34%, and the total yield of glabridin was 71.47%-85.84%. The...

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Abstract

The invention discloses a method for separating an active component-glabridin contained in natural product glycyrrhiza glabra. The method comprises the following steps of: preparing SILMs (Supported Ionic Liquid Membranes) under the pressurizing condition of 0.1-0.3 MPa by selecting ionic liquid; dissolving a crude glabridin product into an ethanol water solution to prepare a glabridin raw material solution; adopting an alkaline water solution as a back extraction solution; fixing the SILMs between a raw material solution pool and a back extraction solution pool, respectively placing two magnetic stirrers at the outer sides of the raw material solution pool and the back extraction solution pool, carrying out the extraction and back extraction of the glabridin under the condition of magnetic stirring, wherein the extraction rate of the glabridin is 70%-90%, and the total recovery rate is 70%-85%. The method disclosed by the invention carries out the separation and purification of the glabridin by adopting a supported ionic liquid membrane technology and realizes the high selectivity, greenness and environmental friendliness of the glabridin.

Description

technical field [0001] The invention relates to a method for separating glabridin by a liquid membrane, in particular to a method for separating glabridin by using an ionic liquid to support a liquid membrane. Background technique [0002] Glabridin is a hydrophobic flavonoid component with significant biological activity in Glycyrrhizae glabra, which has whitening, antibacterial, and antioxidative effects, and has been widely used in the fields of medicine, food, and cosmetics. The content of glabridin in Glycyrrhiza glabra is only about 2 / 1000, and the separation and purification itself is a technical difficulty. At present, the separation and purification methods of glabridin mainly include: solvent extraction-column chromatography technology, solvent extraction-resin adsorption-recrystallization technology. Due to the poor specific selectivity of glabridin and many impurities, it is difficult to separate it with one extraction or one resin, often requiring multiple extr...

Claims

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Application Information

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IPC IPC(8): C07D493/04
CPCC07D493/04
Inventor 郭瑞丽李晓月张建树李雪琴
Owner SHIHEZI UNIVERSITY
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