Glabridin molecularly imprinted polymer, as well as preparation method and application thereof

A technology of glabridin and molecular imprinting, which is applied to chemical instruments and methods, ion exchange, and other chemical processes, can solve the problems of unreported preparation methods and unreported extraction and purification of glabridin molecular imprinting, and achieve good results. The effect of process stability and controllability, strong selectivity and large adsorption capacity

Active Publication Date: 2013-07-31
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the preparation method of glabridin molecularly imprinted polymers, and there is no report on the use of glabridin molecularly imprinted polymers for extraction and purification.

Method used

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  • Glabridin molecularly imprinted polymer, as well as preparation method and application thereof
  • Glabridin molecularly imprinted polymer, as well as preparation method and application thereof
  • Glabridin molecularly imprinted polymer, as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0030] (1) material preparation

[0031] The components of this embodiment are formulated according to the following dosage ratio: template molecule (glabridin) 0.97g, functional monomer (methacrylic acid) 1.63g, crosslinking agent (ethylene glycol dimethacrylate) 19.00g, 0.10 g of initiator (azobisisobutyronitrile), 42.00 g of solvent (mixed solvent with a weight ratio of acetonitrile and chloroform of 1:1).

[0032] (2) Preparation of molecularly imprinted polymers with the template molecule glabridin

[0033] Add the mixed solvent of template molecule glabridin, methacrylic acid, ethylene glycol dimethacrylate, azobisisobutyronitrile, acetonitrile and chloroform prepared in (1) into the reaction kettle and mix evenly, and ultrasonically degas 15min, then filled with nitrogen to remove oxygen, sealed and polymerized in a constant temperature water bath at 60°C for 24 hours to obtain a molecularly imprinted polymer with template molecule glabridin, and then grind it through ...

Embodiment 2

[0040] (1) material preparation

[0041] The components of this example are formulated according to the following dosage ratio: template molecule (glabridin) 0.97g, functional monomer (4-vinylpyridine) 1.83g, crosslinking agent (N,N-dimethylbisacrylamide ) 21.00g, initiator (azobisisobutyronitrile) 0.16g, solvent (N,N-dimethylformamide and chloroform in a weight ratio of 2:1 mixed solvent) 45.00g.

[0042] (2) Preparation of molecularly imprinted polymers with the template molecule glabridin

[0043] Prepare template molecule glabridin, 4-vinylpyridine, N,N-dimethylbisacrylamide, azobisisobutyronitrile, N,N-dimethylformamide and chloroform Add the mixed solvent into the reaction kettle and mix evenly, ultrasonically degas for 15 minutes, then fill with nitrogen to remove oxygen, seal and polymerize in a constant temperature water bath at 60°C for 24 hours to obtain a molecularly imprinted polymer with template molecule glabridin, and then grind it After passing through 100-m...

Embodiment 3

[0050] (1) material preparation

[0051] The components of this embodiment are formulated according to the following dosage ratio: template molecule (glabridin) 0.97g, functional monomer (vinylimidazole) 1.60g, crosslinking agent (ethylene glycol dimethacrylate) 21.00g, 0.16 g of initiator (benzoyl peroxide), 42.00 g of solvent (mixed solvent with a weight ratio of tetrahydrofuran and chloroform of 2:1).

[0052] (2) Preparation of molecularly imprinted polymers with the template molecule glabridin

[0053] Add the mixed solvent of template molecule glabridin, vinylimidazole, ethylene glycol dimethacrylate, azobisisobutyronitrile, tetrahydrofuran and chloroform prepared in (1) into the reaction kettle and mix evenly, and degas it by ultrasonic 15min, then filled with nitrogen and deoxygenated, sealed and polymerized in a constant temperature water bath at 60°C for 24 hours to obtain a molecularly imprinted polymer with template molecule glabridin, which was then ground throug...

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Abstract

The invention relates to a glabridin molecularly imprinted polymer, as well as a preparation method and application thereof. The preparation method comprises the following steps of: taking glabridin as a template, adding the template, functional monomers, a cross-linking agent, an initiator and a solvent into a reaction kettle according to the mass ratio of (1-3): (2-8): (15-30): (0.05-0.2): (20-50), mixing, performing ultrasonic degassing, then charging nitrogen, removing oxygen, sealing, and then polymerizing for 10-24h in a constant-temperature water bath at the temperature of 40-60 DEG C to obtain the glabridin molecularly imprinted polymer with template molecules; and grinding, eluting and drying the obtained polymer to obtain the molecularly imprinted polymer for purifying the glabridin. The obtained glabridin molecularly imprinted polymer can be used as column chromatography packing for column packing so as to be applied in extraction of the glabridin from glycyrrhiza glabra, and the glabridin molecularly imprinted polymer has the advantages of strong selectivity, high adsorption capacity and high purity of the obtained glabridin.

Description

technical field [0001] The invention belongs to the field of preparation of natural products or bioactive components, and in particular relates to a molecularly imprinted polymer for purifying glabridin, a preparation method and application thereof. Background technique [0002] The molecular formula of Glabridin is C 20 h 20 o 4 , with a molecular weight of 324.37, is a natural antioxidant, which is a flavanone compound isolated from the medicinal plant Glycyrrhiza Glabra L. It shows a strong anti-free radical oxidation effect in the cytochrome P450 / NADPH oxidation system, which can significantly inhibit the free radicals produced during the metabolism process in the body, so as to protect against oxidation-sensitive biological macromolecules (low-density lipoprotein LDL, DNA) and cell walls are oxidatively damaged by free radicals. Thereby, some pathological changes related to free radical oxidation can be prevented, such as atherosclerosis, cell aging and so on. At t...

Claims

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Application Information

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IPC IPC(8): C08F222/38C08F222/14C08F212/36C08F220/56C08F220/06C08F226/06C08F220/34C08J9/26B01J20/285B01J20/30B01D15/08
Inventor 王晓陈凌霄纪文华刘峰耿岩玲高乾善刘建华吕瑞敏
Owner SHANDONG ANALYSIS & TEST CENT
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