Coriobacterium sp. AUH-Julong21 and use of coriobacterium sp. AUH-Julong21 in liquiritigenin conversion

An auh-julong21, Antarctica technology, applied in bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as lack of DG resources

Active Publication Date: 2012-03-07
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention solves the problem of lack of DG resources, and provides a method for eff...

Method used

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  • Coriobacterium sp. AUH-Julong21 and use of coriobacterium sp. AUH-Julong21 in liquiritigenin conversion
  • Coriobacterium sp. AUH-Julong21 and use of coriobacterium sp. AUH-Julong21 in liquiritigenin conversion
  • Coriobacterium sp. AUH-Julong21 and use of coriobacterium sp. AUH-Julong21 in liquiritigenin conversion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Isolation and cultivation of strain Julong21

[0051] (1) Collection and culture of human feces samples

[0052] Use a sterilized cotton swab to pick up fresh feces, put it into 1 ml of fresh BHI liquid medium, place it in an anaerobic workstation and cultivate it at 37°C for 24 hours, as a microbial flora for screening specific functional microbial strains;

[0053] (2) Isolation and cultivation of Red Toon fungus AUH-Julong21

[0054] ①Single colony isolation culture

[0055] Use fresh BHI liquid medium to serially dilute the microbial flora that has been cultivated in the anaerobic workstation for 24 hours, and dilute to a concentration of 10 –1 , 10 –2 、10 –3 、10 –4 、10 –5 、10 –6 、10 –7 、10 –8 , and then 100 microliters of concentration were respectively 10 –5 、10 –6 、10 –7 、10 –8 The dilution of microbial flora was uniformly coated on the pre-prepared BHI solid medium, and the BHI solid medium coated with the dilution of microbial flora was placed i...

Embodiment 2

[0084] The isolation method of bacterial strain Julong21 is the same as that in Example 1.

[0085] In the anaerobic workstation, the seed liquid of the above-mentioned pre-cultivated strain Julong21 was transferred to a 250-milliliter Erlenmeyer flask filled with 100 milliliters of liquid medium by 10% inoculum for cultivation, and at the same time, 40 mmol / L of crude glycyrrhizin was added. 0.5 ml (the concentration of crude glycyrrhizinin is calculated based on the peak area value of liquiritinin in crude glycyrrhizin and the standard curve of pure glycyrrhizinin), cultured in an anaerobic workstation for 2 days. Use an equal volume of ethyl acetate to extract the culture in the Erlenmeyer flask twice, filter the extract and evaporate to dryness on a rotary evaporator, then add 100% methanol solution, pass through an organic membrane with a pore size of 0.45 microns, and use a preparative column to perform HPLC analysis. Separation and preparation were carried out on the ab...

Embodiment 3

[0087] The isolation method of bacterial strain Julong21 is the same as that in Example 1.

[0088] In the anaerobic workstation, the seed liquid of the above-mentioned pre-cultivated strain Julong21 was transferred to a 250-milliliter Erlenmeyer flask filled with 100 milliliters of liquid medium by 10% inoculum for cultivation, and at the same time, 60 mmol / L of crude glycyrrhizin was added. 1.3 ml (the concentration of crude glycyrrhizinin is calculated based on the peak area value of liquiritinin in crude glycyrrhizin and the standard curve of pure glycyrrhizinin), cultured in an anaerobic workstation for 3 days. Use an equal volume of ethyl acetate to extract the culture in the Erlenmeyer flask twice, filter the extract and evaporate to dryness on a rotary evaporator, then add 100% methanol solution, pass through an organic membrane with a pore size of 0.45 microns, and use a preparative column to perform HPLC analysis. Separation and preparation were carried out on the ab...

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Abstract

The invention discloses coriobacterium sp. AUH-Julong21 and a use of coriobacterium sp. AUH-Julong21 in liquiritigenin conversion. A preservation number of coriobacterium sp. AUH-Julong21 is china general microbiological culture collection center (CGMCC) NO.5306. A preparation method of coriobacterium sp. AUH-Julong21 comprises the following steps of incubating a coriobacterium sp. AUH-Julong21 strain into a liquid medium for culture, wherein an inoculation rate is in a range of 10 to 15%, adding crude liquiritigenin into the liquid medium, carrying out culture in an anaerobic work station for 2 to 3 days, carrying out culture extraction twice by ethyl acetate, filtering the extract, drying the filtered extract by distillation, adding 100% of a methanol solution into the dried extract, and carrying out product separation preparation in a high performance liquid chromatograph (HPLC) by a preparation column. The coriobacterium sp. AUH-Julong21 can efficiently convert crude liquiritigenin into David viburnum aglycones, and when concentration is in a range of 0.4 to 1.6 millimoles per liter, a 1,1-dipheny-2-picrylhydrazyl (DPPH) extracorporeal clearance rate of the produced David viburnum aglycone is obviously higher than that of substrate liquiritigenin having p less than 0.01.

Description

technical field [0001] The invention relates to a bacterial strain and its application, in particular to a red toon fungus AUH-Julong21 and its application in the transformation of glycyrrhizin. Background technique [0002] Licorice roots is an ancient Chinese medicinal material, which has been recorded in ancient Chinese medical classics and has been used for thousands of years. Licorice has many functions such as protecting the liver, eliminating phlegm and relieving cough, clearing heat and detoxifying, nourishing the spleen and replenishing qi, antibacterial and anti-inflammatory, anti-cancer, hypoglycemic, immune regulation, neuroprotection, etc. It is mainly used clinically to treat the respiratory system, digestive system and immune system and other diseases. Because licorice has good preventive and therapeutic effects on many diseases, especially licorice with low side effects, the research and excavation of pharmacologically active substances in licorice have beco...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/26C12R1/01
Inventor 王秀伶王铭张红蕾
Owner HEBEI AGRICULTURAL UNIV.
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