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Endophytic fungi for producing flavones and application thereof

A technology of flavonoids and strains, applied in the field of microorganisms, can solve the problems of rare functional components produced by endophytes and lack of systematic research

Inactive Publication Date: 2012-10-31
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the existing technology research shows that the research on plant endophytes is mostly focused on the development of their biological control capabilities, while the research on the functional components produced by endophytes is relatively rare, and there are relatively rich endophyte resources in licorice. However, there is still a lack of systematic research on the use of licorice endophytes to produce active products. Therefore, it is of practical significance to study the effective use of licorice endophytes to produce active products

Method used

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  • Endophytic fungi for producing flavones and application thereof
  • Endophytic fungi for producing flavones and application thereof
  • Endophytic fungi for producing flavones and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment one: Penicillium vulgaris ( Penicillium commune )CGMCC NO.6113 Isolation, screening and identification of

[0019] Put the washed and dried roots, stems and leaves of healthy fresh licorice plants into beakers respectively, soak the roots and stems in 2.5% NaClO for 5 min, and soak the leaves for 2 min, rinse with sterile water for 3 times, and then rinse with 75% NaClO. Soak in ethanol for 5 min and 1 min respectively, then rinse with sterile water for 3 to 4 times, and apply the water from the last wash to the medium plate to check whether the surface is thoroughly disinfected.

[0020] The surface-sterilized licorice roots, stems and leaves were respectively ground with a sterile mortar, and a small amount was evenly sprinkled on the separation medium, cultivated in an incubator at 30°C, and observed every day. The streak method was transferred to fresh PDA medium for purification, and finally a pure culture strain was obtained, which was stored on t...

Embodiment 2

[0044] Embodiment two: Penicillium vulgaris ( Penicillium commune )CGMCC NO.6113 of fermentation products

[0045] Select the bacterial strain Penicillium vulgaris ( Penicillium commune ) CGMCC NO.6113, activated on the slant of potato dextrose (PDA) agar test tube, and prepared liquid potato dextrose medium (liquid PDA) at the same time, divided into 150 mL Erlenmeyer flasks according to 50 mL per bottle, and sterilized; the activated Make spore suspension with sterile water on the slant of the strain, and adjust the spore concentration to 10 7 1 / mL, inoculated into the fermentation medium according to the inoculation amount of 10% (v / v), put it in a shaker, and fermented at 30°C and 120 r / min, repeated 5 times, and stopped the fermentation after 6 days. Centrifuge at 6 000 r / min for 15 min to remove the precipitate, and take the supernatant for color reaction detection of flavonoids and determination of flavonoids.

[0046] Flavonoid color reaction detection:

[0...

Embodiment 3

[0058] Embodiment three: Penicillium vulgaris ( Penicillium commune )CGMCC NO.6113 Antioxidant activity detection of fermentation products

[0059] According to the result of embodiment two, it can be determined that Penicillium vulgaris ( Penicillium commune ) CGMCC NO.6113 can produce flavonoids; fermentation is carried out under the same conditions as in Example 2, the fermentation broth is centrifuged, concentrated 5 times, and sterilized by filtration with a 0.22 μm microporous membrane to obtain a crude extract of flavonoids, which is subjected to DPPH· Antioxidant test; DPPH was formulated with 70% ethanol to make 6.5×10 -5 mol / L solution; add samples according to Table 2 in a stoppered test tube, shake well, react at room temperature for 15 min, measure the absorbance at 517 nm, and calculate the clearance rate according to the following formula:

[0060] Clearance (%) = [1-(A i -A j ) / A 0 ]×100%

[0061] Table 3 DPPH·experimental sample addition table ...

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Abstract

The invention discloses an endophytic bacterial strain Penicillium commune CGMC CNO.6113 and an application in the flavone production. Compared with coordinative bacterial strains, the Penicillium commune CGMC CNO.6113 bacterial strain is apparent in the flavone production in mycology. When the bacterial strain is fermented, main ingredients of glycyrrhiza flavonoids of liquiritin, glycyrrhizin, isoliquiritigenin and the like can be produced, and the bacterial strain can be applied in antioxidation, senescence delaying, inflammation elimination and the like as a prevention and treatment method, accordingly, the endophytic fungi for producing flavones and the application thereof provide a novel approach for the development and application of glycyrrhiza flavonoids and have wide applicationvalues.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular, the present invention relates to a flavonoid-producing endophytic fungus and the technical field of its application. Background technique [0002] Licorice ( Glycyrrhiza ) is a perennial leguminous herb and is one of the most commonly used herbal medicines in traditional Chinese medicine in my country. Immunity enhancement and other functions are widely used in medicine and health care, food additives, cosmetics, etc.; licorice is mainly produced in the arid and barren areas of Northeast my country, North China, and Northwest China, and has an important role in sand fixation and greening. Therefore, licorice is listed as a protective medicinal material by the government Indiscriminate digging is prohibited; artificial planting of licorice has developed rapidly in recent years, but because of its long growth cycle and slow income, it still cannot meet the market demand...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/44C12P17/06C12P7/26C12R1/80
Inventor 李艳宾张琴张利莉孟庆艳
Owner TARIM UNIV
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