The invention discloses a production process for jointly separating high-purity
liquiritin, bitterness-removed
glycyrrhizin and
glycyrrhiza uralensis fisch total flavonoids from mono-
ammonium glycyrrhizinate
mother liquor paste. The production process comprises the following steps that (1) the mono-
ammonium glycyrrhizinate
mother liquor paste is taken, water is added to adjust the pH value, the mono-
ammonium glycyrrhizinate
mother liquor paste is dissolved and passes through a resin column 1# to be subjected to
chromatography, water
elution is carried out to obtain eluent for later use, a resin column 2# is subjected to
elution through gradient dilute
ethanol, and after the eluent is concentrated and dried under reduced pressure, a
liquiritin crude product is obtained;
ethanol with the concentration of 95% is added into the crude product, and recrystallization,
filtration and
drying are carried out to obtain the high-purity
liquiritin; (2) the eluent for later use is taken, flows through a resin column 3# and is subjected to water
elution, and the eluent is concentrated and dried to obtain the bitterness-removed
glycyrrhizin; and (3) the resin columns 1#, 2# and 3# are regeneratedby using the
ethanol with the concentration of 95%, eluent is collected and combined, and concentration and
drying are carried out to obtain the
glycyrrhiza uralensis fisch total flavonoids. Accordingto the production process for jointly separating the high-purity liquiritin, the bitterness-removed
glycyrrhizin and the
glycyrrhiza uralensis fisch total flavonoids from the mono-
ammonium glycyrrhizinate mother liquor paste, the mother liquor paste after production of mono-
ammonium glycyrrhizinate is adopted as a
raw material, and the three products are separated and purified in a combined mode,so that the comprehensive utilization degree of the
raw material is improved, the
product value is high, cost is low, reproducibility is good, and the production process is suitable for industrial large-scale production.