115 results about "Echinacoside" patented technology

The tubiflorous desert cistanche preparation containing phenethyl alcohol glycoside contains echinacoside in 10-70 wt% and acteoside in 1-40 wt%. The preparation of the present invention may be applied as active component for preparing medicine treating and preventing senile dementia and for preparing medicine inhibiting platelet aggregation.

The objective of the invention is to provide a photo-aging prophylactic agent for skin with novel ingredients and foods using the photo-aging prophylactic agent for skin, medicaments for mammals including human beings and a skin external agent, as well as feed for mammals. The inventive photo-aging prophylactic agent for skin is characterized by taking extracts of Orobanchaceae plants as effective ingredients. The photo-aging prophylactic agent for skin preferably comprises phenylethanoid glycosides compounds as effective ingredients. Moreover, the photo-aging prophylactic agent for skin preferably comprises at least one of echinacoside and acteoside as the phenylethanoid glycosides compounds. On top of that, the photo-aging prophylactic agent for skin preferably comprises both echinacoside and acteoside as the phenylethanoid glycosides compounds. In addition, the photo-aging prophylactic agent for skin can be employed as medicaments for mammals including human beings, skin external agents, consumptive foods and feed for mammals.

The body of a herbaceous plant, Cistanche tubulosa (Schenk.) Wight, is used to make a medicinal preparation containing phenylethanoid glycosides and comprising 10–70% of echinacoside by weight of the preparation and 1–40% of acteoside by weight of the preparation. The medicinal preparation is used as an active ingredient of medicinal composition for use in prevention of senile dementia, and inhibition of aggregation of blood platelets.

The invention provides a preparation method of a Cistanche tubulosa extract and a skin-whitening anti-aging cosmetic. The preparation method comprises pulverizing, performing crude extraction, centrifugally collecting the obtained crude extract, standing, eluting, and drying to obtain the Cistanche tubulosa extract. The preparation method is simple, and has no pollution. The extract has the content of echinacoside not lower than 25%, the content of verbascoside not lower than 55%, and the content of total glycosides not lower than 80%. Proven by the antioxidant activity test, the Cistanche tubulosa extract has antioxidant, skin whitening and anti-aging effects. The skin-whitening anti-aging cosmetic is prepared by adding the Cistanche tubulosa extract into a cosmetic, is safe and free of stimulation proven by cytotoxicity and safety test, and also has good skin whitening effect.

The invention relates to a preparation method of cistanche deserticola phenylethanoid glycosides. The method comprises the steps of: cistanche deserticola crushing, lixiviating, absorbent resin adsorption, elution, and concentration, etc. In research of the extraction and stability of cistanche deserticola phenylethanoid glycoside, under a weakly acidic condition with pH of 2-4, the cistanche deserticola phenylethanoid glycoside can have substantially improved stability, and under the condition, the cistanche deserticola phenylethanoid glycoside extracted from cistanche deserticola is mainly composed of echinacoside and verbascoside. And in cistanche deserticola phenylethanoid glycosides, the total content proportion of echinacoside and verbascoside is greater than 90%. The content of cistanche deserticola phenylethanoid glycosides extracted by the method of the invention is obviously higher when compared with other extraction methods. And the obtained cistanche deserticola extract has stable effective component content and relative light color. The method provided in the invention avoids decomposition and oxidation of cistanche deserticola phenylethanoid glycoside, and is in favor of product stability.

The invention provides a method that effectively stops and restrains the active ingredients of desertliving cistanche from serious degrading or losing caused by enzyme catalytic reaction initiated during the processing, drying and production technology processes by adopting an improved drying processing manner of the desertliving cistanche. The method is characterized in that: microwave is used for heating and sterilizing enzyme at high temperature, polar molecules contained in the meat stalk cell of fresh desertliving cistanche that is rich in water are led to make high frequency swing, vibration and friction and extrusion between the molecules by using a microwave high frequency electric field so as to lead the temperature inside the cell of the desertliving cistanche and the surface thereof to raise rapidly, thus generating thermal effect that has the function of enzyme sterilization and decoction pieces of the desertliving cistanche is dried rapidly by adopting microwave drying technology, thus effectively preserving the content of the active ingredients of phenylethanoid glycosides, etc. that is rich in the meat stalk of the fresh desertliving cistanche and greatly promoting the quality and medical value of the prepared decoction pieces of the desertliving cistanche. Upon analysis and determination, the content of the active ingredients of the phenylethanoid glycosides, etc. contained in the prepared decoction pieces, which is in the shapes of filiform, elongated flake or granular, of the desertliving cistanche is rich, the content of the main index ingredients of echinacoside and verbascoside monomeric compounds of the prepared decoction pieces of the desertliving cistanche, which used for weighing the quality thereof, is up to 13-24.1 percent (being calculated based on dry substance) and the water content thereof is 1.2-3.8 percent.

The present invention relates to an extraction and separation method for echinacoside and verbascoside in cistanche. The method comprises the following steps: dicing the cistanche and drying the cistanche; adding 75% ethanol into a multi-functional ultrasonic extraction tank to extract for three times; combining extraction liquid and concentrating the extraction liquid until no ethanol exists; filtering and clarifying the extraction liquid and the concentration liquid of the cistanche by using ultrafiltration equipment; performing absorption on the liquid by using a macroporous resin column; firstly washing the liquid with water until the liquid is colorless, then eluting the liquid by using ethanol with certain concentration; collecting an elution solution that contains the echinacoside and the verbascoside; concentrating the elution solution by using an outer circulating vaccum concentrator; carrying out spray-drying on the concentration liquid to obtain the cistanche extract; and carrying out efficient liquid-phase tracing detection on the cistanche extract, wherein the echinacoside accounts for greater than or equal to 25%, and the verbascoside accounts for greater than or equal to 10%. The total content of phenylethanoid glycosides is greater than or equal to 80%, and the yield is 20%.

The invention provides a production method, which can enable the content of Phenylethanoid Glycoside combination before and after the processing to keep stable through improving the processing treatment technology of a broomrape decoction piece, wherein, the Phenylethanoid Glycoside combination is the active ingredient abundant in broomrape. Through a high-frequency microwave electrical field, the invention leads a polar molecule in a succulent stem cell of a waterrich fresh broomrape to perform high-frequency swing, vibration, and intermolecular friction and extrusion to cause the inner and surface temperature of a broomrape cell to rise rapidly; the pyrometric effect for inactivating the enzyme activity, so as to effectively block and restrain the degradation loss of the Phenylethanoid Glycoside combination caused by the enzymatic reaction induced by enzyme systems such as hydrolytic ferment, glucoside hydrolase, etc., abundant in a fresh broomrape cell; at the same time, the broomrape decoction piece is dried through the technology for microwave drying or other drying methods. The analysis determination shows that the content of echinacoside and a verbascoside monomer compound which are the main index components for weighing the quality of the broomrape decoction piece in the acquired broomrape decoction piece can reach 13 percent to 24 percent (by dry matters), and the water content is 1.3 percent to 4.1 percent.

The invention provides a system integration technology through cell disruption, slurry extraction, membrane separation and macroporous resin adsorption and chromatography separation and purification, high temperature enzyme deactivation and spray drying and the like, which achieves a technical process that from the time fresh cistanche raw materials are fed until obtaining active matters such as monomeric compounds which are rich in echinacoside and verbascoside and the like in 30 to 80 minutes, and the invention effectively overcomes a technical defect that active components such as benzyl carbinol glycosides compounds are normally seriously degraded and lost, which are caused by an enzymatic reaction which is initiated by an imperfect dry treatment method of cistanche, and the invention obviously improves the medical value of cistanche. In the active maters of cistanche which are obtained ( calculating by dry matter), the productivity of echinacoside monomeric compound reaches to 9.8% to 19.6%, and the yield of verbascoside monomeric compound reaches to 2.0% to 7.5%. The content of echinacoside monomeric compound in active matters of cistanche which are obtained reaches to 15% to 49.6%, and the content of verbascoside monomeric compound reaches to 5% to 26.7%.

The invention provides application of echinacoside or acteoside in preparation of angiogenesis inhibitor medicines. Tests prove that angiogenesis echinacoside and acteoside can be used for effectively inhibiting reproduction of vascular endothelial cells, can be used as angiogenesis inhibitors for treating tumors, diabetic retinopathy, rheumatoid arthritis, prostatic hyperplasia or psoriasis and other angiogenesis dependent diseases and have a wide market prospect.

The invention relates to application of echinacoside in anti-tumor medicaments. The application provided by the invention is as follows: firstly adding MTH1, inorganic pyrophosphatase and dGTP into a reaction solution (100mM pH 8.0 Tris-acetic acid, 40mM NaCl, 10mM magnesium acetate, 0.005% Tween 20 and 1mM DTT), incubating an enzyme and a substrate at room temperature for 1h, then adding 25 mu l of malachite green solution to terminate reaction and performing absorbance detection at 630nm by using an iMark microplate reader, wherein results show that echinacoside has a significant effect of inhibiting enzyme activity of MTH1; and secondly detecting the effects of echinacoside against tumor cells at the cellular level by an MTT experiment, wherein the results show that echinacoside can obviously inhibit the growth of SW480 colon cancer cells and U2OS human osteosarcoma cells. According to the application provided by the invention, echinacoside is used for inhibiting specific enzyme MTH1 for maintaining survival in the tumor cells, and oxidized nucleotide is mixed into DNA, thereby resulting in fatal DNA double-strand break in the cancer cells, producing an anti-tumor effect, finding a new medical use of echinacoside and laying a foundation for future development of the new anti-tumor medicaments.

The invention provides a cistanche deserticola medicinal material processing method including the following steps: 1) impurities in a fresh Cistanche deserticola medicinal material are removed, sediment is rapidly flushed away with water, the fresh Cistanche deserticola medicinal material is cut into thick slices; 2) high temperature enzyme killing process of the thick slices obtained in step 1 is performed for 1 and 15min; 3) after heat treatment the thick slices are lyophilized. Cistanche deserticola decoction pieces processed by the method are white in color, beautiful in appearance and dry in texture, and the content of main effective constituents of echinacoside and verbascoside is 10 times greater than that of a natural drying method.

The invention provides a healthcare product containing cistanche tubulosa extract and a preparation method thereof. The healthcare product contains a cistanche tubulosa extract, a wolfberry extract and auxiliary materials in weight percentage; the cistanche tubulosa extract contains echinacoside as an active ingredient. Fine powders of the screened cistanche tubulosa extract, wolfberry extract and auxiliary materials are mixed uniformly to obtain dispersed healthcare products; the dispersed healthcare products are pressed to obtain tablet cores and then are coated to obtain healthcare tablets. According to the invention, raw materials capable of enhancing immunity and relieving physical fatigue are selected to prepare the healthcare product with functions of enhancing immunity and relieving physical fatigue; the healthcare product is good in stability and plays a role of enhancing the body resistance to diseases and delaying and relieving fatigue, so that the healthcare product raises the health level of people, improves the quality of life and work, and has a certain degree of social and economic benefits. The healthcare product is suitable for people with low immunity and unsuitable for children.

The present invention discloses an echinacea purpurea extract. The echinacea purpurea extract comprises 2.4 to 3.1% of caftaric acid, 4.4 to 5.7% of chicoric acid and 1.5 to 2.0% of echinacoside based on the weight percentages of all ingredients accounting for the dry weight of the extract. The present invention also discloses a preparing method of echinacea purpurea extract and a detection method for phenols ingredient content in echinacea purpurea extract. The phenols ingredient content in the echinacea purpurea extract all meet the requirements of the active ingredient content specification of echinacea purpurea in United State Pharmacopeia 30 (USP 30). The phenols ingredient content is increased by 4 to 6% than that of echinacea purpurea extract sold on market. The preparing method has the advantages of simple technique, low cost, low energy consumption and easy industrialization.

The present invention provides a production method of cynamorlum extract full of active ingredients as echinacoside and veroascose glucoside monomeric compounds with high yield extracted from fresh cynamorlum succulent stem. The production method is implemented through systematic integrated technology of cytoclasis, serous extraction, membrane separation, high-temperature enzyme sterilization, spraying and drying, and other processes, so as to realize the manufacturing process from feeding the fresh cynamorlum to obtaining powdery or granular cynamorlum extract with high yield within 15 to 60 minutes. The present invention effectively inhibits, blocks and overcomes the technical defect of serious degradation and loss of active ingredients of echinacoside and veroascose glucoside monomeric compounds caused by enzyme catalysis reaction of poor drying method of cynamorlum. The major index ingredients of the obtained powdery or granular cynamorlum extract for measuring the cynamorlum quality are echinacoside and veroascose glucoside monomeric compounds, the productive rate of which is up to 15 to 23.4 percent (calculated in dry materials).

The invention provides a preparation method of a molecularly imprinted membrane for separation of phenylethanoid glycosides. The method includes the steps of: adding acteoside and a functional monomerinto a solvent, performing sealing, conducting mixing, and carrying out pre-polymerization; and adding ethylene glycol dimethacrylate and azodiisobutyronitrile into the pre-polymerized system to obtain a polymerization system, immersing a PVDF membrane into the polymerization system, carrying out nitrogen bubbling deoxygenation, then carrying out polymerization reaction to obtain an imprinted membrane, and eluting template molecules in the imprinted membrane with an eluent to obtain the molecularly imprinted membrane. According to the invention, by applying the molecularly imprinted membraneto extract active ingredients in cistanche tubulosa, the adsorption capacity of acteoside reaches 62.82mg / g, the adsorption capacity of echinacoside reaches 29.49mg / g, and the selectivity is 2.74. Inpermselectivity experiment, the permeability coefficient of acteoside is 4.02*10<-6>cm<2> / s, the permeability coefficient of echinacoside is 1.07*10<-5>cm<2> / s, and the permeation selectivity factor is 2.65.

The present invention relates to the technical field of health-care foods, and relates to a composition containing herba cistanches and herba epimedii extracts, and a preparation method and an application thereof. The composition containing the herba cistanches and herba epimedii extracts is prepared from the following raw materials in parts by weight: the herba cistanches extract, the herba epimedii extract, a nutritional supplement, microcrystalline celluloses or mannitol, magnesium stearate and a flavoring agent. Compared with echinacoside and verbascoside total amount and the herba epimedii content in the existing health-care products, those of the composition containing the herba cistanches and herba epimedii extracts are greatly improved. Besides, compared with a traditional Chinese medicine of America ginsengs, the composition containing the herba cistanches and herba epimedii extracts has better effects in relieving body fatigues. At the same time, the composition containing the herba cistanches and herba epimedii extracts has better improvements in blur vision, frequent yawns, dizziness, shoulder stiffness, slow response and attention decline caused by driving fatigues.

The invention relates to an application of echinacoside in the preparation of drugs for treating Parkinson's disease. The invention discloses that echinacoside has a remarkable protective effect against the MMP+ mediated cell injury. The echinacoside can be used to prepare drugs for the treating Parkinson's disease and offers a new treatment for people with the Parkinson's disease.

The invention belongs to the field of traditional Chinese medicine extraction, and especially relates to a preparation method of high purity cistanche phenyl ethanol glycosides and an application of high purity cistanche phenyl ethanol glycosides in the preparation of drugs for treating pulmonary fibrosis. The preparation method extracts two high purity cistanche phenyl ethanol glycosides from cistanche. The invention specifically discloses a preparation method and applications of high purity cistanche phenyl ethanol glycosides. Three resin columns are used together; Amberlite XAD16HP macroporous resin is used to remove impurities such as sugar; Amberlite FPC22Na (macroporous strongly acidic styrene resin) and D201 (strong alkali I type anion exchange resin) are used to effectively removepigments and components that are not phenyl ethanol glycosides; three resins are screened from a plurality of resins, the extracted cistanche phenyl ethanol glycosides have a light yellow color; the content of total phenyl ethanol glycosides is more than 95% (weight ratio), the content of echinacoside and verbascoside is more than 85% (weight ratio), and the phenyl ethanol extract has a good effect on preventing pulmonary fibrosis.

The invention relates to a cultivation method for improving the yield and the quality of Cistanche deserticola. Annual Haloxylon ammodendron saplings are selected, and are planted in desertified loess in spring or autumn according to a planting density of 0.15-0.52 plant / m<2>, humus soil is applied according to a ratio of 1000-2000 g / plant, the planted samplings are timely watered to ensure the survival rate, inoculation with a Cistanche deserticola seed mixture is carried out after the Haloxylon ammodendro saplings grow to about 1.5 m, the humus soil is applied again according to a ratio of 500-2000 g / plant, the inoculation time is March to May Or October to December every year, the Cistanche deserticola seed mixture comprises Cistanche deserticola seeds subjected to seed soaking treatment in a seed soaking solution, sucrose, L-tyrosine, L-phenylalanine and mint powder, the seed soaking solution is a 3-5 mg / L fluridone solution, the seed soaking treatment time is 12-36 h, and the Cistanche deserticola seeds subjected to seed soaking treatment are obtained through drying the soaked seeds in the shade. The cultivation method has the following advantages: the inoculation process of the Cistanche deserticola seeds is simple, and can effectively promote the germination of the Cistanche deserticola seeds and effectively improve the yield of the Cistanche deserticola planted in the desertified loess; and the composition of the Cistanche deserticola seed mixture can be screened to obviously improve the content of effective components comprising echinacoside and verbascoside in the artificially planted Cistanche deserticola, improve the quality of the artificially planted Cistanche deserticola and effectively enhance the drug effects and the economic benefits of the artificially planted Cistanche deserticola.

The invention discloses a fresh cistanche decoction piece and a preparation method thereof. The preparation method comprises fresh cistanche selection, cleaning and enzyme killing: picking and cleaning fresh cistanche, putting the cistanche into a drug moistening machine at a temperature of 86-93 DEG C and carrying out steam treatment in the drug moistening machine for 20-30min, drying: putting the cistanche into a baking box, carrying out baking at a temperature of 60 DEG C for 4-6h, transferring the cistanche into a sun-drying shed and carrying out drying in the shade, wherein in drying in the shade, the cistanche is turned twice every day, cutting, drying: carrying out drying at a temperature of 55-65 DEG C for 4-6h, and sorting and package. The preparation method directly treats fresh cistanche in a producing area so that the treated raw material cistanche has no dry head or has dry head content of 5% or less and the whole cistanche can be used as decoction pieces. An experiment proves that compared with the prior art, the preparation method improves verbascoside and echinacoside content by 2-5 times and improves polysaccharide content.

The invention discloses an application of echinacoside (ECH) in preparation of a medicine for treating neuropathic pain. Experimental results show that when the ECH is used in a safe dosage range, theECH with the dosage of 200 mg / kg can remarkably relieve allodynia and hyperalgesia induced by a sciatic nerve chronic compression injury model, improve histopathological changes of sciatic nerves andregulate protein expression of inflammatory cytokines; and it is proved that the ECH has the effects of relieving the neuropathic pain caused by chronic compression injury and promoting the neural functional recovery.

The invention relates to a method for inspecting the quality standards of a Conghuang kidney-reinforcing preparation, which used for controlling the quality of the Conghuang kidney reinforcing preparation, wherein, the preparation refers to granules, tablets and hard gelatin capsules. When disclosing the method for inspecting the quality standards of a new Conghuang kidney reinforcing preparation, relatively great improvement is made to the existing methods for inspecting the quality standards of medical pills by the invention, which is particularly characterized in that: thin layers qualitative differential diagnosis of prepared rhizome of rehmannia, seed of Chinese dodder and fruit of Chinese magnolia vine (steaming with wine) in a prescription is added, the preparation method of desertliving cistache differential diagnosis sample solution is changed and determination of echinacoside with little interference but high content by high performance liquid chromatography is modified. The quality controllability of the new Conghuang kidney-reinforcing preparation can be improved by using the inspecting method of the invention, which is favorable to the quality stability of products.

The invention provides a plateau sunscreen cream containing a natural product--echinacoside. A preparation method for the plateau sunscreen cream comprises the following steps: separately heating oil phase components (including stearic acid, monoglyceride stearate, Vaseline, nanometer zinc oxide and octyl methoxycinnamate) and water phase components (including triethanolamine, glycerin, azone, echinacoside, ethylparaben, vitamin C and deionized water) to 80 DEG C; adding a molten oil phase into a water phase and carrying out stirring at a high speed; cooling a cream body to 40 DEG C and carrying out homogenization and degassing; and continuing slow stirring until the cream body is formed after complete emulsification and condensing the cream body to obtain the plateau sunscreen cream containing echinacoside. The plateau sunscreen cream provided by the invention has good protection effect when applied to treatment or prevention of skin injury caused by high-intensity plateau ultraviolet rays.

The invention provides a new application of echinacoside. Natural micromolecule active compound echinacoside has selective peculiar protection effect on injury of nerve cells, the echinacoside can activate TrkA / B receptors, and the nerve protection effect of the echinacoside can be reversed by restraining the receptors from being activated through Trk receptor specificity inhibitors. Vein medication of the echinacoside can generate obvious nerve protection effect, and simultaneously long-time vein medication causes no obvious liver and renal toxicity.

The present invention discloses pharmaceutical use of Echinacoside (ECH) for treatment of hypoxic-ischemic encephalopathy. The experimental results of the present invention indicate that the Etoposideat a dose of 160 mg / kg can significantly improve cerebral infarction volume and neuronal damage and apoptosis in a hypoxic-ischemic model of neonatal rats when used in a safe dose range, and prove that the Etoposide can promote the recovery of neuronal ischemia and hypoxia injury and prevent disability and death after hypoxic ischemic encephalopathy.

The invention relates to a method for producing mullein glucoside monomeric compound, which comprises the following steps: carrying out high-temperature enzyme deactivation and cell rupture on fleshystem fresh cistanche deserticola or cistanche deserticola decoction pieces; taking beta-glucosidase industrial enzyme preparation as biological catalyst; carrying out biocatalysis reaction by a solidstate fermentation reactor; rupturing glucose residue ligand terminal by echinacoside zymolyte in catalyzed hydrolysis substrate (belt-1,6-glycosidic bond); directionally translating into high-activity mullein glucoside monomeric compound; and then carrying out systematic process treatment, such as serosity extraction, separation and purification, concentration and drying and the like to obtain the mullein glucoside monomeric compound. By adjusting separation and purification process conditions, the purity percentage content of the meal or drying material of the obtained mullein glucoside monomeric compound can reach 35.8%-89.5%. The mullein glucoside monomeric compound has the characteristics of micromolecule and low polarity, can show good absorptivity and special pharmacology activity in drug absorption.