Echinacea purpurea extract and preparing method and phenols ingredient detection method thereof
A technology of Echinacea purpurea extract and Echinacea purpurea, applied in the directions of plant/algae/fungus/moss components, medical preparations containing active ingredients, material separation, etc., can solve problems such as hidden dangers of food safety, unsuitability for industrial production, and high production cost. , to achieve the effect of high recovery rate, low cost and obvious effect
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Embodiment 1
[0040] The preparation of embodiment 1 Echinacea purpurea extract
[0041] Take 2 kg of dried medicinal materials of Echinacea purpurea, crush them into 40 meshes, add 8 times the amount of 40% ethanol aqueous solution for reflux extraction for 0.5 hours, pour out the extract, add 10 times the amount of 10% ethanol aqueous solution for reflux extraction for 1 hour, and combine all the extracts, Concentrate under reduced pressure to 1 / 3 of the original volume, centrifuge at 4000r / min for 10min, pour out the supernatant, add 2 times the volume of the supernatant to dilute with deionized water, and filter the diluted solution through an organic membrane with a pore size of 300nm. The pressure is 0.2MPa, the operating temperature is 28°C, and 50L of the organic membrane permeate is obtained. The organic membrane permeate is separated by the ultrafiltration membrane. The membrane pore size is 10nm, and the operating pressure is 0.6MPa. The ultrafiltration membrane filtrate is then ...
Embodiment 2
[0042] The preparation of embodiment 2 Echinacea purpurea extract
[0043] Take 2 kg of dried medicinal materials of Echinacea purpurea, crush them into 40 meshes, add 10 times the amount of 50% ethanol aqueous solution for reflux extraction 3 times, each time for 0.5 hours, pour out the extract, add 10 times the amount of 10% ethanol aqueous solution for reflux extraction of the residue for 1 hour, Combine all the extracts, concentrate under reduced pressure to 1 / 3 of the original volume, centrifuge at 4000r / min for 10min, pour out the supernatant, add 2 times the volume of the supernatant to dilute with deionized water, and filter the dilution through an organic membrane first. The membrane pore diameter is 300nm, the operating pressure is 0.2MPa, and the operating temperature is 28°C to obtain 50L of organic membrane permeate, which is then separated by ultrafiltration membrane. Liquid 41L. The ultrafiltration membrane filtrate is then concentrated by nanofiltration membra...
Embodiment 3
[0044] The preparation of embodiment 3 Echinacea purpurea extract
[0045] Take 2 kg of dried medicinal materials of Echinacea purpurea, crush them into 40 meshes, add 12 times the amount of 50% ethanol aqueous solution for reflux extraction for 2 hours, pour out the extract, add 10 times the amount of 10% ethanol aqueous solution for reflux extraction for 1 hour, and combine all the extracts, Concentrate under reduced pressure to 1 / 3 of the original volume, centrifuge at 4000r / min for 10min, pour out the supernatant, add 2 times the volume of the supernatant to dilute with deionized water, and filter the diluted solution through an organic membrane with a pore size of 300nm. The pressure is 0.2MPa, the operating temperature is 28°C, and 50L of the organic membrane permeate is obtained. The organic membrane permeate is separated by the ultrafiltration membrane. The membrane pore size is 10nm, and the operating pressure is 0.6MPa. The ultrafiltration membrane filtrate is then c...
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