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Application for liquiritin and derivatives of liquiritin in preparation of drugs for treating and/or preventing novel coronaviruses

A technology of coronavirus and liquiritin, applied in the field of pharmacy, can solve problems such as no drugs with good therapeutic effects have been found

Inactive Publication Date: 2020-06-19
PEKING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, scientists have carried out a large number of drug screening experiments against the 2019 novel coronavirus, however, no drug with good therapeutic effect has been found

Method used

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  • Application for liquiritin and derivatives of liquiritin in preparation of drugs for treating and/or preventing novel coronaviruses
  • Application for liquiritin and derivatives of liquiritin in preparation of drugs for treating and/or preventing novel coronaviruses
  • Application for liquiritin and derivatives of liquiritin in preparation of drugs for treating and/or preventing novel coronaviruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Safety Assessment of Liquiritin (Liquiritin) on Cells

[0036] Vero cells were inoculated into 96-well cell culture plates at a concentration of 10,000 / well, and cultured overnight at 37°C and 5% CO2. When the cells grow to a 50% monolayer, add 100 μL of 2% FBS DMEM maintenance solution containing different concentrations of liquiritin (the initial concentration is 400 μM, and the drug to be tested is diluted with a 3-fold dilution gradient, a total of 8 concentrations) / well, three replicate wells were measured for each concentration. Continue to culture and observe the cell state under the microscope every day. On the 4th day after adding the drug, add 20 μL of MTS solution, incubate at 37°C and 5% CO2 for 1 hour, and measure the OD490 value.

[0037] The formula (1-(drug group OD-medium OD) / (cell control group OD-medium OD))×100% was used to calculate the cytotoxicity (%) of different concentrations of drugs to Vero cells, and the data were processed by G...

Embodiment 2

[0039] Example 2 Liquiritin detects the half maximum effective concentration (EC50) of SARS--Cov--2

[0040] The EC50 of the drug was determined by nucleic acid quantification method. details as follows:

[0041] Vero cells were inoculated into 48-well plates at a concentration of 10,000 cells / well one day in advance. The drug was formulated into different concentrations with DMEM maintenance solution of 2% FBS, added to the cells, and placed in a 5% CO2 incubator at 37° C. for 1 hour for pretreatment. Then, add 100TCID50SARS-CoV-2 virus solution, adsorb and culture at 37°C for 2 hours, discard the virus solution, add different concentrations of liquiritin (200μl / well) to each well, and set up virus control and normal cells in 3 duplicate wells. The control group was cultured in a 5% CO2 incubator at 37°C, and cytopathic changes (CPE) were observed every day. Two days after infection, 50 μl of cell supernatant was taken from each well to extract nucleic acid, and the viral ...

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Abstract

The invention provides an application for liquiritin and geometric isomers, derivatives or pharmaceutically-acceptable salts and / or solvates and / or hydrates of the liquiritin in preparation of drugs for treating and / or preventing novel coronaviruses. According to the invention, the liquiritin is found to be able to effectively inhibit the novel coronaviruses SARS-CoV-2 at a cellular level, whereinEC50 is 2.39 [mu]M. The liquiritin is indicated to have positive effects on treatment and / or prevention of the novel coronaviruses SARS-CoV-2.

Description

technical field [0001] The invention belongs to the field of pharmacy, and particularly relates to the application of liquiritin and its derivatives in the preparation of anti-new coronavirus drugs for treatment and / or prevention. Background technique [0002] In terms of systematic classification, coronaviruses belong to the genus Coronavirus (Coronavirus) of the order Nidovirales (Nidovirales) and are single-stranded positive-sense RNA viruses. Viruses of the genus Coronavirus are RNA viruses with an envelope and a linear single-stranded positive strand genome. They are a large class of viruses that exist widely in nature. [0003] The diameter of coronavirus is about 80-120nm, the 5′ end of the genome has a methylated cap structure, and the 3′ end has a poly(A) tail. The total length of the genome is about 27-32kb. . [0004] Coronaviruses can infect vertebrates and humans and cause various diseases such as respiratory infections and gastroenteritis, such as humans, mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P31/14A61P11/00A61P31/16A61P31/20A61P31/18
CPCA61K31/7048A61P11/00A61P31/14A61P31/16A61P31/18A61P31/20
Inventor 谢正伟秦成峰邓永强李晓峰张娜娜朱杰王昕
Owner PEKING UNIV
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