472 results about "Penicillium adametzii" patented technology

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

The invention discloses a nano silver antibacterial latex pillow or mattress and a preparation method thereof. Tiny air holes are fully distributed on the surface of the nano silver antibacterial latex pillow or mattress, and air is filled into the tiny air holes; and the silver antibacterial latex pillow or mattress has bacterial resistance on aspergillus niger, aspergillus flavus, discolored aspergillus, penicillium citrinum, paecilomyces varioti, herbarium cladosporium, trichoderma viride and chaetomium globasum. The preparation method comprises the following steps of: uniformly mixing nano sliver with natural latex; then respectively adding sulphur, bi(triethoxy propylsilane)tetrasulfide and 2,6-butylethylene-4-methylphenol; uniformly stirring; adding soap and air to generate foams; then adding gelatin; and compressing for moulding. The nano silver antibacterial latex pillow or mattress of the invention combines air permeability, high elasticity and soft touch feeling of the latex with efficient bacterial resistance and broad spectrum sterilizing performance of nano silver and can effectively overcome the defects of easy bacterial generation, difficult air permeation, and the like of a common pillow on the market.

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergilllus host. In a specific example, the specific performance of the formulation using puriified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

The invention relates to novel anti-tumor application of an alkaloid compound penicillium enol A1 from penicillium citrinum. The penicillium citrinum IBPT-5 is collected in the China Center for Type Culture Collection (CCTCC), which is located in Wuhan University and has the collection number of CCTCC NO:M2013713, on December 25, 2013. Experiments prove that the compound has better anti-tumor activity on various tumor cells, and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for anti-tumor study, wherein tumor cells comprise human colon cancer cells SW620, human hepatoma cells Huh7, human gastric carcinoma cells BGC-823, human colon cancer cells SW480, human esophageal squamous carcinoma cells KYSE450, human esophageal cancer cells EC9706, human highly metastatic lung carcinoma cells 95-D, human hepatoma cells PLC and human gastric carcinoma cells HGC-27.

The invention provides penicillium ochrochloron Y5 and a microbial inoculum thereof for efficiently degrading crop straw. High-yield cellulase of the penicillium ochrochloron Y5 can efficiently degrade straw fiber lignin, is provided with the capacity of degrading multiple crop straw and enjoys a very good degradation effect on straw of wheats and corns, particularly on cellulase and hemicellulose of wheat straw. The cellulase of the penicillium ochrochloron Y5 has the activity higher than the activity of reported strains, thus being suitable for rapid degradation of rice straw in south of China and wheat and corn straw in north of China and enjoying the functions of promoting in-situ biodegradation and field returning among straw fields and increasing the content of organic matters.

The invention relates to a bactericidal composition containing prothioconazole, which contains active ingredient A and active ingredient B, wherein active ingredient A is selected from prothioconazole, and active ingredient B is selected from thiophanate-methyl, multibacterium One of Ling and prochloraz, wherein the weight ratio of active ingredient A to active ingredient B is 1:80 to 80:1, made into wettable powder, water dispersible granule, suspending agent, suspoemulsion, water emulsion, Microemulsion, used to control powdery mildew, sheath blight, fusarium wilt, leaf spot, rust, sclerotinia, viral disease, anthracnose, gray mold, ring spot, bakanae disease, rice blast, early blight, Diseases such as green mold, pedicle rot, penicillium, flax spot, scab, blight, etc. have the characteristics of small dosage, resistance to rain erosion, and obvious synergistic effects.

The invention provides a composite microbial biogen capable of degrading organic waste efficiently and a preparation method and applications thereof. The composite microbial biogen contains Bacillus circulans, Bacillus subtilis, Alcaligenes faecalis and Penicillium diversum. The composite microbial biogen can be used with activated sludge for the existing sewage treatment equipment; under the synergetic effect, the yield of excess sludge can be greatly reduced, the aims of emission reduction and energy saving can be realized and the sludge problem which is difficult to overcome for a long time in sludge treatment is effectively solved; and the biogen has importanct economic importance and environmental importance and is suitable for each sewage treatment plant. The composite microbial biogen can also be used to prepare organic wastewater purifying agents, activated sludge enhancers of urban domestic wastewater and deodorants used in tipping sites, public places, agricultural product market, kitchens, bathrooms and the like.

The invention discloses a method for improving production of a lignocellulosic enzyme line from filamentous fungi and an application of the filamentous fungi in fermentation of microbial bio-based chemicals, and belongs to the field of genetic engineering. According to the method, high-affinity glucose transporter of the filamentous fungi is transformed by virtue of such genetic engineering approaches as knocking-out, mutation, importing and the like, and an obtained engineering strain has the advantages. The filamentous fungi include, but not limited in, neurospora, myceliophthora, trichoderma, aspergillus, penicillin, fusarium and the like; genes of the transporter include NCU10021 (HGT-1) and a homologous gene thereof as well as NCU04963 (HGT-2) and a homologous gene thereof; any one or two genes are knocked out, or bases of any one or two genes are mutated, or expression of any one or two genes is decreased. The engineering strain, which is obtained by importing the transporter into a microbial strain in an exogenous mode or by changing endogenous expression of the transporter, can gain or enhance a glucose transporting and utilizing capacity, and subsequently, a capacity of producing such bio-based chemicals as organic acid, organic alcohol and the like through fermentation can be gained or improved.

The invention discloses application of fermentation liquor of bacillus subtilis (CICC No. 10321) in inhibition of growth of penicillium and aspergillus niger. The bacillus subtilis (CICC No. 10321) plays a role in inhibiting penicillium and aspergillus niger (the penicillium and aspergillus niger inhibiting circle is greater than 20 cm), the inhibition effect is good, and the duration is long. The bacillus subtilis is simple in culture, short in cycle and low in cost, is suitable for serving as a mold inhibitor and is used for inhibiting the growth of penicillium and aspergillus niger in easily-mildewed goods, such as feed and the like, and the residues of mycotoxins in animal foods are reduced effectively.

The invention relates to novel anti-tumor application of an alkaloid compound penicillium enol B1 from penicillium citrinum. The penicillium citrinum IBPT-5 is collected in the China Center for Type Culture Collection (CCTCC), which is located in Wuhan University and has the collection number of CCTCC NO:M2013713, on December 25, 2013. The compound has good anti-tumor activity to various tumor cells, and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for anti-tumor study, wherein tumor cells comprise human colon cancer cells SW620, human hepatoma cells Huh7, human gastric carcinoma cells SGC-7901, human gastric carcinoma cells BGC-823, human colon cancer cells SW480, human esophageal cancer cells EC9706, human highly metastatic lung carcinoma cells 95-D, human hepatoma cells PLC, human gastric carcinoma cells HGC-27 and human lymphoma cells RAJI.

The invention discloses a strain penicillium oxalicum with high-efficiency phosphate solubilizing characteristic, which is classified and named as Penicillium oxalicum. The penicillium oxalicum has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number: CGMCC NO.7699. The invention further discloses tolerance of high-efficiency phosphate-solubilizing penicillium oxalicum to heavy metals. The strain has strong dissolving effect on insoluble phosphate tricalcium phosphate, magnesium phosphate and aluminum phosphate under the condition of shake-flask liquid culture condition, the dissolving effect on the tricalcium phosphate and magnesium phosphate can reach 100%, and the dissolving effect on the aluminum phosphate can reach 95.5%. The penicillium oxalicum is respectively inoculated into liquid culture liquor containing heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>, has strong resistance to the heavy metals, especially, the concentration of tolerance to the Pb<2+>, Zn<2+>, Cr<2+> and Mn<2+> can respectively reach 2000mg / L. Therefore, the penicillium oxalicum can provide excellent strain resources for developing heavy metal-resistant high-efficiency phosphate-solubilizing bacteria agents.

The invention discloses a preparation method of a nano-silver composite antibacterial agent. The invention is characterized in that the nano-silver composite antibacterial agent is formed by combining a nano-silver solution and an organic antibacterial agent which have synergistic effect and adduction effect according to a certain ratio. The structure and performance of the antibacterial agent after combination are stable, and the antibacterial agent has more rapid, efficient and broad-spectrum antibacterial performance. The minimal inhibitory concentration (MIC) of the antibacterial agent for inhibiting escherichia coli, Enterobacter hormaechei, staphylococcus aureus, bacillus subtilis, Rhodotorula mucilaginosa, penicillium and Aspergillus niger is only 1-10 microgram / ml. The preparation method of the nano-silver composite antibacterial agent is characterized in that the nano-silver in the nano-silver composite antibacterial agent has monodispersity and there is no agglomeration phenomenon. The preparation method has advantages of ingenious conception, simple experimental operation, high operationality and short period, and is suitable for large-scale production.

The invention provides a lactobacillus plantarum AB-3 strain having an activity of resisting phytophthora melonis kalsura, of which the accession number is CGMCC No.9655. The lactobacillus plantarum AB-3 has effective inhibition properties on escherichia coli, salmonella typhimurium and shigella flexneri, has effective inhibition properties on the phytophthora melonis kalsura, fusarium oxysporum, penicillium roqueforti and glomerella cingulata and is excellent in thermostability. A fermentation supernate of the strain shows a significantly increased bacteria inhibition rate after treatment at 100 DEG C and is enhanced in bacteria inhibition activity through enzymolysis by a neutral protease. The fermentation supernate of the strain has a storage resistance property and is excellent in bacteria inhibition activity even after normal storage for 20 days.

The invention discloses a heavy metal biological absorbent, a preparation method of the absorbent and an application in treating cadmium-containing wastewater. The heavy metal biological absorbent mainly consists of penicillium simplicissimum bacteria powder after being modified by rhamnolipid. The method for preparing the biological absorbent comprises the following main steps: culturing penicillium simplicissimum in a liquid culture medium, then continuously enlarging and culturing the cultured penicillium simplicissimum, adding the rhamnolipid taken as biosurfactants during the process, collecting fungus balls after the culture, cleaning the fungus balls with inorganic salt solution, freezing and drying the fungus balls to the constant weight and grinding the fungus balls into powder to obtain the heavy metal biological absorbent. In the application in treating the cadmium-containing wastewater, the heavy metal biological absorbent is mainly utilized to remove the cadmium in the wastewater, and the method mainly comprises the following steps: adding the heavy metal biological absorbent into the cadmium-containing wastewater, carrying out oscillatory reaction and completely removing after the filtration. The heavy metal biological absorbent has good absorption effect when being used for treating the cadmium-containing wastewater. The method for removing the cadmium contained in the wastewater has the advantages of simple operation conditions, low cost and high efficiency and is easy to implement.

The invention provides a seedling-cultivating substrate capable of preventing and treating soil-borne diseases and a production method thereof. The substrate is formed by compounding main material compositions, auxiliary material compositions and additives. The main material compositions comprise, by volume, 70-85% of cassava residues, 5-15% of peat, 5-10% of vermiculite and 5-10% of perlite. The auxiliary material compositions comprise, by weight part in the mixture of the main material compositions per cubic meter, 5-15kg / m<3> of sheep manure, 0.5-1.5 kg / m<3> of calcium nitrate, 0.5-1.5 kg / m<3> of slow release fertilizer and 2-4kg / m<3> of ferrous sulfate. 0.5-2.5 kg of solid penicillium inoculants, namely the additives are added in the combined main material compositions and the auxiliary material compositions per cubic meter by volume. The production method of the substrate comprises the steps of preparation of raw materials, the compounding process, adding of the additives and preparation of finished products. As proven by practical application, the seedling-cultivating substrate which is made of waste cassava residues as the major raw material and is capable of preventing and treating the soil-borne diseases is good in quality and low in cost, and the pollution-free seedling-cultivating substrate is provided for the sustainable development of agriculture.

The invention discloses a penicillin antibiotic aptamer obtained by the screening technology for an improved aptamer without a fixed point target substance in the field of screening a penicillin antibiotic aptamer without a fixed point target substance, and an application thereof. The unfixed nucleic acid is eluted through a fixed oligonucleotide library; the parent nucleus 6-APA (6-aminopenicillanic acid) of the penicillin antibiotic is added in positive screening to act on the fixed nucleic acid; after eluting the nucleic acid molecules which can be combined with 6-APA, PCR (polymerase chain reaction) amplification is directly performed for next screening; and other antibiotics are added in negative screening, and the nucleic acid molecules in non-specific binding with the penicillin antibiotics are removed by elution. Through multiple rounds of positive and negative screening, 10 nucleic acid aptamers with high specificity and strong affinity with 6-APA are obtained; and the nucleic acid aptamer with a stable secondary structure is selected for developing a nucleic acid aptamer sensor for detecting 6-APA.

The invention discloses a method for improving the efficacy of cryptococcus laurentii with control on the penicilliosis and the patulin of post-picked apples, belonging to the technical field of biological control. The method is carried out according to following steps: activating the cryptococcus laurentii, inoculating the cryptococcus laurentii to a trehalose supplemental medium (a carbon source comprises 0.4% of trehalose and 0.6% of glucose) for culturing, and centrifuging the cryptococcus laurentii to obtain thalli; preparing the thalli into a 1*108 pieces / ml bacterium suspension; and adding 30mul yeast bacterium suspensions to the wounds of fruit, naturally drying the fruit, putting the fruit into a plastic basket, sealing by plastic wraps, and storing the fruit at a room temperature. Using the trehalose to improve the efficacy of the cryptococcus laurentii with control on the penicilliosis and the patulin of the post-picked apples, the method for improving the efficacy of the cryptococcus laurentii with control on the penicilliosis and the patulin of the post-picked apples is friendly to environment, safe, economical, efficient and applicable, thereby having important social values and economic values.

The invention provides a preparation method of ginsenoside CK and relates to a method for preparing 20-O-beta-D-glucopyranosyl-20-protopanoxadiol (ginsenoside CK) by fermenting and converting protopanoxadiol in a full-automatic fermentation tank by means of a bacterial strain. The method comprises the following steps: (1) culture of microorganisms; (2) fermentation and conversion of microorganisms; and (3) chromatographic separation and purification by macroporous resin to finally obtain a compound with the chemical name: 20-O-beta-D-glucopyranosyl-20-protopanoxadiol (ginsenoside CK).

The invention relates to penicillium protein elicitor EPTP and application thereof in improving plant disease resistance. The protein sequence of the EPTP protein elicitor is shown as SEQ ID NO.2, and the molecular weight is 19247 Da. EPTP induces significant increase of activity of phenylalanine ammonialyase, peroxidase and polyphenol oxidase, and when the elicitor induces tobacco anaphylactic reaction, an anaphylactic reaction marker gene hin1 is induced to express; it is indicated through semiquantitative RT-PCR results that EPTP can induce tobacco to obtain systemic acquired resistance, cause up-regulation of the tobacco blade resistance gene transcription level, and induce expression of resistance gene related to the salicylic acid path and the jasmonic acid path. After being applied, EPTP can improve resistance to viruses and pathogenic bacteria by plants, takes effects efficiently, does not pollute environment, reduces dosage of chemical pesticide and has wide application prospects as a plant immunity activator.

The invention provides a fermentation method for preparing nuclease P1, which using directly penicillium citrinum sporogon as seed, fermenting by means of fed-batch sugar supplement to improve activity of the nuclease P1, the mehod without first and second order seed culture shorts production cycle greatly and raises efficiency. The invetion also uses obtained enzyme for preparing 5'-mononucleotide with 85-90% rate of enzymatichydrolysis and 15 mg / ml of producing rate of nucleotide. The invention also provides a penicillium citrinum 3.2788-Y01 of nuclease P1, which fungus stroing number is CGMCC NO.1943, the stem can raise one times active unit of the nuclease P1.

A (1R, 5S, 6S)-6-((1R)-1-ethoxyl)-2-((3S, 5S)-5-sulfonamide-amino-methyl-pentazane-3-base) sulfur-1-methyl-1-carbon-2-penicillic vinyl-3-carboxylic acid or its hydrate, its medicinal composite containing multi-nipenan crystal, its production and use in preparation of anti-infectious medicine are disclosed. In the crystallizing powdery X-ray diagram, it has main peak at diffraction angle (2 theta)=6.46, 15.27, 16.41, 17.49, 20.72, 23.05 and 25.38 deg. minus or plus 0.1. It's simple and cheap, and has excellent thermodynamic stability and dissolubility.

The invention belongs to the preparation of a new gene, and in particular discloses a glucose oxidase gene GOD, protein coded by the GOD and pichia pastoris transformed by the GOD. The glucose oxidase gene GOD is prepared by taking 5'-ATGAAGTCCACTATTATCACCTCCA and 3'-CTAGGCACTTTTGGCATAGTCTTCA as specific primers and penicillium notatum as a template through polymerase chain reaction (PCR) amplification. According to the invention, the problem of an existing recombinant glucose oxidase gene in heterologous expression is solved; the total-length GOD gene and a shuttle expression vector constructed by the gene are obtained and the gene and the shuttle expression vector thereof are further transformed to the pichia pastoris, and upon screening and identification, a strain, which is higher in secretion expression of the glucose oxidase than an original strain, is obtained. The invention can greatly save cost and expense for further industrialized production and can improve the economic benefits of the glucose oxidase.

The present invention provides a Pichia pastoris for highly efficient heterologous expression of white Penicillium cyclopium lipase and an enzyme production medium. According to the present invention, the white Penicillium cyclopium lipase containing a self leader peptide (7 amino acids) is heterologously expressed in a genetically engineered Pichia pastoris, increasing the activity of exogenous protein lipase, and improving a part of enzymatic properties of the exogenous protein lipase. According to the enzyme production medium of the invention, through optimizing the YNB component of a BMMY medium, the medium cost is reduced by 70%. According to the invention, reliable experimental basis is provided for the industrial application of the enzyme, especially for the catalyzed hydrolysis of substrate astaxanthin ester from Haematococcus pluvialis to produce astaxanthin monomers.

The invention belongs to the field of microorganism, and particularly relates to a microorganism penicillium strain producing high-activity cellulase. The penicillium strain has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M2011195. The invention also provides an application of the strain in cellulose enzymatic hydrolysis. Through mutation breeding, the penicillium strain producing high-activity cellulase is bred; cellulase crude enzyme liquid is obtained through fermentation of the strain, which has filter paper enzyme activity of up to 10 IU / ml, endoglucanase activity of up to 30 IU / ml, exoglucanase activity of up to 1.5 IU / ml and beta-glucosidase activity of up to 8 IU / ml, respectively being 3 times, 4 times, 4.5 times, and 4 times higher than the activities of an original strain; when the strain is applied to enzymatic hydrolysis of cellulose raw materials, the cellulose conversion rate in 3 days is up to more than 80%, and the cellulase has extremely high activity.

The invention discloses a low salt pickling and artificial fermentation process of Xuanwei ham, the technical proposal includes four steps of raw material selection, low salt pickling, fermentation fungi group cultivation and artificial fermentation, and a fermentation fungi group comprises Penicillium, lactic acid bacteria, Bacterium lacticum and Micrococcus in the ratio of 2.7:0.73:0.2:1. The advantages are that after raw material meat is inoculated with the lactic acid bacteria, the lactic acid bacteria uses carbohydrates such as glucose for fermentation to produce lactic acid, gelatinous tissues are formed by degeneration of muscle proteins under acidic conditions, bonding force among meat blocks is increased, and hardness and elasticity of meat products are improved, the lactic acid bacteria has high salt tolerance, can be used for fermentation of sugar to produce the lactic acid to play antibacterial and hypoglycemic effects and improve meat freshness; the Micrococcus has effects of decomposition of proteins and fat and reduction of nitrates, large amounts of amino acids are produced after the proteins are broken down, the amino acids are sources of a fresh taste, meanwhile by reduction of the cancerogenic substance nitrates, health of food customers can be protected.

Disclosed in the present invention is a fungicidal composition containing prothioconazole and a prochloraz manganese salt and use thereof. The fungicidal composition uses prothioconazole and a prochloraz manganese salt as the main active ingredients. The fungicidal composition may be applied to control cereal, fruit, and vegetable diseases, and has high synergistic effects, overcomes and delays the resistance of pathogenic fungi, and has a fast fungicidal speed and long duration. The application cost is reduced, and the control effect is significantly better than that of a single agent. The present invention may be used to control fungal diseases on crops, and in particular, can be used to control powdery mildew, sheath blight, fusarium wilt, leaf spot, rust disease, stalk break, net blotch, scald disease, gray mold, black spot, brown blotch, black shank, cucumber, citrus and pimento anthracnose, citrus stem-end rot, green mold, penicilliosis and mushroom brown rot, and the effects are better than that of a single agent.

The invention discloses a method for producing a particle board through anti-insect and anti-mold composite urea formaldehyde resin. The method comprises the procedures like material preparation, drying, veneer removing and grit removing, mixing glue, and hot press molding. The key of the production method is that melamine modified urea formaldehyde resin is adopted as a main material of an adhesive; oxidized starch is added as a viscous complexing agent; polyvinyl alcohol is adopted as a flexibilizer; hydrogenated rosin is adopted as an antioxidant; polyisocyanate is adopted as a curing agent; and triethoxyisobutylsilane is adopted as a bridging agent. According to the method, the anti-insect and anti-mold composite urea formaldehyde resin is prepared through adopting phenolated lignin as a formaldehyde capture agent and adding an anti-insect and anti-mold agent, and the particle board produced through the anti-insect and anti-mold composite urea formaldehyde resin has the advantages that the residual amount of formaldehyde is low and the anti-insect and anti-mold effect is good, has a quite high inhibiting effect on breeding of aspergillus, trichoderma and penicillia, and has instantaneous killing force and a long-term prevention effect on board powder-post beetles.

Crude enzyme preparations are respectively obtained by a method for centrifugation, concentration, ammonium sulfate precipitation, freezing and drying of fermenting medium solutions for bacillus subtilis and penicillium raistrickii, and are mixed with fungal laccase through a specific component ratio, so that a compound enzyme preparation for efficiently degrading aflatoxin B1 is prepared. The bacillus subtilis crude enzyme preparation and the penicillium raistrickii crude enzyme preparation are mixed with the fungal laccase, the respective component ratio occupied is controlled to be 20-60 percent, and the sum is 1. The prepared compound enzyme preparation can efficiently degrade the aflatoxin B1, and the degrading interval is 76-89 percent. Under the same treatment conditions, the degrading efficiency of the compound enzyme preparation is improved by 25 percent or above as compared with that of each of the three components for the aflatoxin B1. When the ratio of the compound enzyme preparation to the aflatoxin B1 in a system is controlled to be 0.2-0.4 g / L.ppm, compared with any component in the compound enzyme preparation, the compound enzyme preparation shows significant material-saving property.

The invention relates to a novel fluopicolide-containing antibacterial composition. The novel fluopicolide-containing antibacterial composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is fluopicolide; the active ingredient B is one of thiophanatemethyl and carbendazim; the weight ratio of the active ingredient A to the active ingredient B is 1:80-60:1; and wettable powder, water dispersible granules, suspending agents, suspending emulsion, water emulsion and microemulsion can be prepared by adding aids into the active ingredients. The novel fluopicolide-containing antibacterial composition is used for preventing and controlling downy mildew, epidemic disease, late blight, dampingoff, powdery mildew, botrytis, early blight, fusarium wilt, sclerotiniose, anthracnose, ring spot, penicilliosis and green mould of crops, can resist rain wash and has obvious synergism.