Method for preparing nuclease P1 by ferment process

Abstract

The invention provides a fermentation method for preparing nuclease P1, which using directly penicillium citrinum sporogon as seed, fermenting by means of fed-batch sugar supplement to improve activity of the nuclease P1, the mehod without first and second order seed culture shorts production cycle greatly and raises efficiency. The invetion also uses obtained enzyme for preparing 5'-mononucleotide with 85-90% rate of enzymatichydrolysis and 15 mg / ml of producing rate of nucleotide. The invention also provides a penicillium citrinum 3.2788-Y01 of nuclease P1, which fungus stroing number is CGMCC NO.1943, the stem can raise one times active unit of the nuclease P1.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a production and preparation of nuclease P 1 Methods. Background technique [0002] Nucleic acid degradation products—nucleotides and their derivatives are widely used in food, medicine, agriculture, cosmetics, and biochemical reagents (Chen Taosheng, Modern Industrial Microbiology Volume 1, Shanghai Science and Technology Press, 1979, 153-164 ), while nuclease P 1 It is the main enzyme for enzymatically hydrolyzing RNA and DNA into 5'-nucleotides. [0003] In the 1960s, in order to develop the monosodium glutamate market, Japan began to obtain flavor enhancers—flavored nucleotides (5’-GMP and 5’-IMP, etc.) from degraded RNA. In 1957, Japanese scholar Guo Zhongming and others screened a strain of Penicillium citrinum (Agric Biol Chem 25, 693 (1961)), which can secrete an extracellular enzyme with a phosphodiesterase activity. Function, enzymatically digests RNA to generate 5'NMP....

AI Technical Summary

Problems solved by technology

In the original production method, whether solid or liquid culture is used, the unit of enzyme activity is low, only 100-150 units / ml, and the fermentation period is long, and the production cost is high.

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