The invention discloses a compound
microbial agent of
nematode egg parasitical fungi and a
spore germination accelerating agent, and a preparation method and application of the compound
microbial agent. The compound
microbial agent of the
nematode egg parasitical fungi and the
spore germination accelerating agent comprises
paecilomyces lilacinus
spore powder or
fermentation broth and the
spore germination accelerating agent. The best combination with various
nutrient substances, which can be used for promoting the
germination of
paecilomyces lilacinus spores, is obtained through orthogonal experiments, and the bio-
control effect of the compound microbial agent for promoting the germination of the
paecilomyces lilacinus spores and controlling root-knot nematodes is verified through indoor
bioassay experiments. After the compound microbial agent acts on the root-knot nematodes for five days, the egg
parasitism rate of the root-knot nematodes can reach more than 80 percent, and the egg hatchability of the root-knot nematodes is almost zero, which are obviously superior to those of a control group. Experimental results prove that the
spore germination accelerating agent added in the compound microbial agent can be used for effectively promoting
spore germination, so that the
parasitism rate of
paecilomyces lilacinus to root-knot
nematode eggs is improved, the acting time is shorted, and finally the
control effect on the root-knot nematodes of plants is significantly improved.