323results about How to "Improve anti-bacterial ability" patented technology

The invention relates to a yeast for producing tyrosine and hydroxytyrosine and a construction method, and belongs to the technical field of preparation of organic compounds. The construction method comprises the following steps of introducing the sequences PcAAS and ADH into the yeast BY4741, so as to obtain the recombinant yeast PcAAS-ADH for producing the tyrosine; introducing a pdc1 gene knockoff cassette and a tyrA expression cassette into the recombinant yeast PcAAS-ADH, so as to obtain the recombinant yeast PcAAS-ADH-deltapdc1-tyrA for producing the tyrosine; introducing the DNA (deoxyribonucleic acid) sequence of HpaBC into the recombinant yeast PcAAS-ADH-deltapdc1-tyrA, so as to obtain the recombinant yeast PcAAS-ADH-HpaBC-deltapdc1-tyrA for producing the hydroxytyrosine. The tyrosine or hydroxytyrosine biological synthesis route is constructed in the yeast BY4741, so that the output of the tyrosine or hydroxytyrosine is increased.

The invention discloses a breeding method for natural ganoderma lucidum, and belongs to the technical field of edible fungus genetic breeding. The breeding method comprises the following steps of 1 natural ganoderma lucidum collecting, 2 test tube slant culture medium preparing, 3 tissue separating, 4 strain inoculating and cultivating, and 5 inoculating and cultivating. The natural ganoderma lucidum separated by the breeding method is large in mother strain shape, high in yield and high in sundry fungus resisting capacity, the cultivation and expanding problem of the natural ganoderma lucidum can be solved, and the finished product yield is high.

The invention provides a preparation method of a special antibacterial paint for a hospital and relates to the preparation method of the antibacterial paint. The antibacterial paint is prepared from deionized water, hydroxyethyl cellulose, dispersing agents, wetting agents, mould inhibitors, pH regulators, defoaming agents, anti-freezing agents, titanium dioxide or precipitated barium sulphate, inorganic antibacterial materials, rheology modifying agents, kaolin, light calcium powder, film forming auxiliary agents, acrylic emulsion and alkali swelling thickening agents. The method comprises the following steps of: weighing raw materials; mixing and stirring the deionized water, the defoaming agents, the dispersing agents and the hydroxyethyl cellulose; adding the wetting agents, the mould inhibitors, the anti-freezing agents, the titanium dioxide or the precipitated barium sulphate, the rheology modifying agents, the kaolin, the light calcium powder and the alkali swelling thickening agents to be continuously stirred; then, adding the inorganic antibacterial materials, the acrylic emulsion and the pH regulators; stirring the materials; next, adding the film forming auxiliary agents; obtaining pulp after the stirring; and grinding the pulp to obtain the special antibacterial paint. The special antibacterial paint for the hospital has the excellent bloodstain resistance and contamination resistance performance, the advantages of strong-effect antibacterial and mould inhibition effects, environment friendliness and the like are also realized, the antibacterial performance is high, and the durability is good.

The invention provides a compound bacterium agent for treating green wastes of gardens as well as a preparation method and application of the compound bacterium agent. The compound bacterium agent isprepared from phanerochaete chrysosporium, trichoderma viride, geotrichum candidum, candida utilis, saccharomyces cerevisiae, bacillus mucilaginosus, bacillus subtilis, lactobacillus plantarum, streptococcus thermophiles and azotobacter chroococcum. When the compound bacterium agent provided by the invention is used for treating the green wastes of the gardens, a carbon-nitrogen ratio and a carbon-phosphorus ratio are adjusted, without the need of adding any auxiliary material; a whole compost fermentation process can be finished through only independently adding the compound bacterium agent,so that the treatment cost of the green wastes of the gardens is effectively saved and a treatment technology is simplified. Microorganisms in the compound bacterium agent can be rapidly and greatly reproduced in a fermentation process, and rapid temperature raising and ageing of compost are promoted; the treatment time of the green wastes of the gardens is shortened and the compound bacterium agent is suitable for treating the green wastes of the gardens in practice.

The invention discloses an immune feed additive, which is prepared from maggots, wherein the maggots are fed with fermented leftovers from aquatic product processing by enzymolysis. The invention also provides a method for preparing the immune feed additive, which comprises the following steps: 1, fermenting the leftovers from aquatic product processing; 2, feeding maggots with the fermented leftovers from aquatic product processing; 3, performing the enzymolysis of the maggots to obtain the immune feed additive. The feed additive provided by the invention contains rich nutrients and functional elements and can promote growth and obviously improve immunity and pathogenic bacterium resistance in animals; therefore, the feed additive is a high-efficiency immune feed additive. The preparation method of the feed additive adopts a biological technique, avoids producing any waste in a production process, and is green, environmentally-friendly, simple in process, high in maneuverability and capable of allowing for large-scale production.

The invention discloses a vegetable planting method. The method comprises the following steps of step 1, culturing seedlings: mixing nitrogen-fixing bacteria, bacillus subtilis and fish meal to obtain a first mixture, uniformly mixing one part of the first mixture and distilled water to obtain first mixed liquor, mixing the other part of the first mixture and a substrate to obtain a second mixture, adding the second mixture into a cell pan, placing vegetable seeds into the cell pan containing the second mixture for culturing seedlings, after seedlings grow out, spraying the seedlings with the first mixed liquor, and transplanting after seedling cultivation; step 2, transplanting: planting the vegetable seedlings on ridge back; step 3, field management. According to the vegetable planting method, the first mixed liquor containing the nitrogen-fixing bacteria and the bacillus subtilis is adopted for spraying the vegetable seedlings during a seedling culturing period, the morbidity of fungal diseases such as vegetable damping off and late blight is reduced by combining the nitrogen-fixing bacteria and the bacillus subtilis added in the substrate, the effect of preventing and treating vegetable diseases is realized, and the pollution to environment is smaller than that of traditional chemicals.

The invention discloses a straw mushroom cultivation method which refers to a technology of applying mushroom roots into straw mushroom cultivation. Compared with the prior art, the straw mushroom cultivation method disclosed by the invention has the advantages that 1, a mushroom root leaching solution refers to a pure natural nutrient solution and contains bioactive substances of rich varieties, the mushroom root soaking materials and cultivation raw materials are blended for performing stacking fermentation, the fermentation effect can be improved, and the fermented material has an effect of promoting growth of straw mushroom mycelium, thereby guaranteeing strong mycelium growth vigor and high infectious microbe capacity; and 2, after the mushrooms are harvested, the mycelium renovation effect is good by using the mushroom root leaching solution, the leaching solution has the effects of promoting straw mushroom primordium formation and growth of sporocarp, the yield can be obviously improved, the problem that the quality of the straw mushrooms is reduced because insecticides, bactericides and chemically synthetic fertilizers are used for a long time is solved, and the quality of the straw mushrooms is improved.

The invention discloses penicillium purpurescens QL-9204 and application for preparing phloretin during phlorizin conversion. The penicillium purpurescens QL-9204 is a strain free of toxicity and harm and safe to use. The penicillium purpurescens QL-9204 grows rapidly, and is high in hybrid bacteria resistance, easy to culture and stable in batch; a fermentation medium is simple in components and low in market price, thereby being low in production cost; a substrate plorizin is directly put into a crude enzyme for conversion, the enzyme separation and purification steps are omitted, the technology is simple, and the penicillium purpurescens QL-9204 is easy to apply industrially; the phloretin conversion yield is high and can reach 92.5% to the maximum, and the penicillium purpurescens QL-9204 has the advantages that few by-products are produced, and the product is easy to extract.

The invention relates to a culture medium for large scale production of photosynthetic bacteria, particularly aiming to solve the problem that when the photosynthetic bacteria are cultured in high-temperature weather in summer, blue (green) algae are generated to result in instable quality. The invention further solves the problems of non-bright thallus color, high cost and low thallus concentration caused by using high-concentration yeast extract for producing the photosynthetic bacteria. The culture medium for the photosynthetic bacteria is composed of NH4CL (or NH4HCO2), CH3COONa, KH2PO4, MgSO4, CaCL2, CH3CH2OH, and trace bacillus, yeast powder, trace elements and sterile water; and the photosynthetic bacteria cultured by using the culture medium has the quality guarantee period reaching more than two years.

A mushroom cultivation process, wherein peanut shell raw material is selected as the culture medium for artificial cultivation which comprises, disintegration, sieving, germicidal treatment, vaccination of mushroom bacterial suitable for cultivation, controlling temperature within the range of 15-20 deg C., the mushroom end product can be obtained within 7-15 days. The advantages of the invention are, rich raw material resource, simple process for manufacturing, early mushroom production, high antibiotic capability and high production.

The invention relates to a preparation method of a mushroom cultivation material, and relates to a technology that mushroom root is applied to the mushroom cultivation. Compared with the prior art, the preparation method has the advantages that firstly, a mushroom root leaching agent is a pure natural nutrient solution, contains various kinds of rich bioactive substances, a mushroom root soaking material is mixed with a cultivation material for carrying out piling fermentation, beneficial microorganism reproduction is facilitated, a microbial flora in the material is improved, and catalytic decomposition of the cultivation material is accelerated; the fermented cultivation material has high-activity probiotics and active metabolic products, and has the accelerating effect on the growth of mushroom hypha, by adopting the fermented cultivation material, the hypha has high growth vigor and strong infectious microbe resisting ability; and thirdly, resources such as labor and coal are saved, the environment pollution is reduced and the method is in accordance with the low-carbon environment-protection concept.