77results about How to "Does not inhibit growth" patented technology

The invention relates to a probiotic complex against helicobacter pylori and a preparation method of the probiotic complex. The probiotic complex is prepared from the following raw materials: 2 to 10parts by weight of lactobacillus helveticus, 10 to 25 parts by weight of bifidobacterium animalis, 5 to 15 parts by weight of bifidobacterium bifidum, 10 to 25 parts by weight of lactobacillus acidophilus, 2 to 15 parts by weight of lactobacillus rhamnosus, 1 to 6 parts by weight of bifidobacterium bifidum, 5 to 15 parts by weight of soy protein powder, 50 to 100 parts by weight of inulin, 20 to 50 parts by weight of resistant dextrin, 20 to 50 parts by weight of fructose-oligosaccharide, 2 to 5 parts by weight of flos lonicerae water extract and 10 to 20 parts by weight of prunus armeniaca fruit. The complex can achieve the purpose of eradicating the helicobacter pylori; in addition, the complex has the advantages of being free of side effects, fast in effect taking, thorough in treatmentin the use process and free of recurrence in a year after treatment.

The invention relates to a bacterial strain for preventing potato blight and a preparation method and the application of a preparation thereof. The bacterial strain is bacillus subtilis MES810, the preservation date is Aug, 10th, 2017, and the preservation number is CGMCCNo. 145154. The preparation method of the preparation corresponding to the bacterial strain comprises the following steps: activation of a culture; preparation of a primary seed solution; preparation of a secondary seed solution; fermentation in a fermentation tank, and discharging to obtain a bacillus subtilis liquid preparation. The bacillus subtilis has a strong inhibiting effect for fusarium pathogenic bacteria consisting of Fusarium solani, fusarium oxysporum, fusarium moniliforme, Fusarium nivale or elderberry fusarium and Eavenaceum, the occurrence of potato blight is prevented, the control efficiency is more than 80%, and the increase of both production and income for potato is promoted. The bacterial strain disclosed by the invention has the characteristics of no toxicity, no residue, no growth inhibition, small using amount and simple application method, and provides the possibility for the diversification of application schemes and the protection of ecological environment.

The invention discloses a photosynthetic bacteria culture medium, relates to a bacteria culture medium, and solves the problems that the existing photosynthetic bacteria culture media contains high-concentration yeast extract that leads to darker lysate color and high production cost, and pH value of the culture medium rises along with the prolonging of cultivation time, thus inhibiting the growth of photosynthetic bacteria and leading to lower bacteria concentration. The photosynthetic bacteria culture medium is made of low molecular weight carbonaceous organic substances, ammonium salts, the yeast extract, MgSO4, CaCl2, K2HPO4, KH2PO4, trace elements and distilled water. As the photosynthetic bacteria culture medium takes the low molecular weight carbonaceous organic substances that do not contain Na<+> as carbon source, pH value of the culture medium does not rise along with the prolonging of cultivation time, and the growth of photosynthetic bacteria is not inhibited; low-concentration yeast extract is adopted to reduce production cost, and the trace elements ensure bright photosynthetic bacteria lysate color; photosynthetic bacteria cultivated by the culture medium has the exponential growth phase of two days, thus having high bacteria concentration and the OD660 of 1.8.

The invention discloses a removing method of hexavalent chromium in the effluent in the anaerobic / aerobic batched reactor through active sludge microbe in the environment protective technical domain, which comprises the following steps: placing active sludge in the SBR; adding effluent with Cr (VI) into the reactor; stirring 1-6h under anaerobic condition; aerating for 1-6h under aerobic condition; sedimenting 1-2h; making the density of hexavalent chromium and total chromium in the outlet supernatant reach the first grade national discharge standard; reducing the harm of environment and human due to chromium; enriching trivalent chromium in the sludge with low toxicity; operating the chromium removing system continuously without inhibiting the growth of microbe.

The invention relates to a nifuratel-nystatin soft capsule-type suppository and a preparation method thereof. Nifuratel-nystatin sol in the nifuratel-nystatin soft capsule-type suppository is preparedfrom the following components in parts by weight: 40-60 parts of nifuratel, 3-5 parts of nystatin, 2-3 parts of carbomer, 1-2 parts of ethylparaben, 50-70 parts of glycerinum, 70-100 parts of ethyl alcohol, 20-30 parts of pH buffer liquid and 700-800 parts of medical pure water. The preparation method comprises the following steps of 1, raw material preparation; 2, liquid medicine preparation, wherein the nifuratel and the nystatin are mixed evenly in a sterile environment, then through a sterile filtering system, sterile filtering is conducted, and then micro-pore filtering is conducted, sothat a liquid medicine is obtained for later use; 3, gel matrix preparation; 4, nifuratel-nystatin sol preparation; 5, capsule pressing; 6, soft capsule-type suppository preparation. The nifuratel-nystatin soft capsule-type suppository and the preparation method thereof have the advantages that body hormone secretion is not affected, the sterilization effect is good, and the preparation method issimple.

Relating to the technical field of microorganisms, the invention provides a bacillus coagulans MES847, a microbial inoculum, a chicken feed, a preparation method and application. The bacillus coagulans MES847 provided by the invention is preserved in China General Microbiological Culture Collection Center on August 30, 2018, and the preservation number is CGMCC NO.16358. The bacillus coagulans MES847 has a wide antibacterial spectrum, can be used for preparation of products preventing and / or treating chicken diarrhea, at the same time can improve the cellular immunity and humoral immunity level of organisms, stimulates the development of animal immune organs, and improve the disease resistance of animals.

The application belongs to the field of medicine and discloses novel application of lotus plumule extracts, in particular to application of lotus plumule extracts in the preparation of inhibitory drugs for the quorum-sensing system. Experiments show that the lotus plumule extracts can inhibit the biological membrane of each of three species of Pseudomonas aeruginosa; that the lotus plumule extracts can inhibit the quorum-sensing system so as to decrease bacterial virulence and pathogenicity without inhibiting growth of bacteria, while bacterial drug resistance is rarely caused. Particularly all lotus plumule chloroform layer extract, lotus plumule ethyl acetate layer extract, lotus plumule total alkaloids extract and lotus plumule total flavones extract is as effective as or more effectivethan the positive drugs, furanone compounds, in inhibiting the biological membranes of Pseudomonas aeruginosa and drug-resistant Pseudomonas aeruginosa; it is indicated that the lotus plumule extracts provide good inhibition for both Pseudomonas aeruginosa and drug-resistant Pseudomonas aeruginosa.

The invention provides a method for driving pigs to move during breeding. The pigs are concentrated in a ring-shaped running field. If there is an unpleasant smell, the pigs will flee quickly. The circular running field is restricted by fences. The pigs will run along the track planned by the fence. The width of the track is 1.8‑2.5m until they reach the reward area; (2) The width of the reward area will It suddenly becomes larger, with a width of 5-6m. After the pigs run to the reward area, they will disperse and then stop running. Then the experimenters will feed the pigs and reward the pigs until they are full to the seventh to eighth level. The live pigs bred by this method have excellent meat quality, moderate water content, increased lean meat ratio, overflowing aroma after cooking, do not use a large amount of vaccines and antibiotics, improve the resistance of the pigs through exercise, and produce healthy and reliable meat pigs.

The invention relates to a culture medium for large scale production of photosynthetic bacteria, particularly aiming to solve the problem that when the photosynthetic bacteria are cultured in high-temperature weather in summer, blue (green) algae are generated to result in instable quality. The invention further solves the problems of non-bright thallus color, high cost and low thallus concentration caused by using high-concentration yeast extract for producing the photosynthetic bacteria. The culture medium for the photosynthetic bacteria is composed of NH4CL (or NH4HCO2), CH3COONa, KH2PO4, MgSO4, CaCL2, CH3CH2OH, and trace bacillus, yeast powder, trace elements and sterile water; and the photosynthetic bacteria cultured by using the culture medium has the quality guarantee period reaching more than two years.

The invention provides a fig leaf extract with bacterium colony induction quenching activity, and a use thereof, and concretely relates to a preparation method of a fig leaf crude extract, a bacterium colony induction quenching activity screening method, and an application of the extract in the preparation of bacterium colony induction inhibitors. The bacterium colony induction quenching activity of the fig leaf extract is reported for the first time in the invention. The fig leaf crude extract prepared through extraction using organic solvents with different polarities has an inhibition effect on Chromobacterium violaceum and Pseudomonas aeruginosa colony induction system, wherein a dichloromethane extract has strongest colony induction system quenching activity. The crude extract can substantially reduce the output of colony induction mediated Chromobacterium violaceum purpurin and the flocking motion of the Pseudomonas aeruginosa and weaken the pesticide resistance of pathogenic bacteria, and can be used to prepare novel bacterium colony induction inhibitors, so the fig leaf extract has wide application prospect in prevention and control of bacterial infective diseases.

The present application relates to an aspartokinase mutant, a microorganism including the mutant, and a method for production of an aspartate-derived L-amino acid or homoserine derivative, using the microorganicm.

The invention provides an implant for the orthopedics department, a material for preparing the implant and a preparation method of the implant. The material for preparing the implant is formed by compositing glass fiber serving as a reinforcing phase and polylactic acid serving as a base phase. The preparation method of the implant comprises the steps as follows: step 1, preparing the glass fibercontaining a polylactic acid molecular chain structure; step 2, compositing the glass fiber and polylactic acid and then performing processing. The compatibility and dispersion uniformity of the glassfiber and the polylactic acid base body are improved, the strength of the polylactic acid implant is improved effectively, the stress shielding effect can be avoided, and the implantation effect is good.

The invention discloses an application of benzyl benzoate in preparation of bacterial colony induction activity inhibitors; in the method, an oceanobacillus sp. XC22919 fermented product is used for separation and extraction, and in a concentration range of 0-60 [mu]g / mL, bacterial growth of chromobacterium violaceum CV026 is not inhibited, but generation of chromobacterium violaceum purpurin canbe significantly decreased; and with gradual increase of the concentration, the effect of inhibiting generation of the purpurin is stronger; the effect of the compound in inhibiting colony induction is in a concentration dependence. In a concentration range of 0-100 [mu]g / mL, bacterial growth of pseudomonas aeruginosa PAO1 is not affected, but the expression of virulence factors of pseudomonas aeruginosa can be significantly reduced, the expression of pyocyanin, elastase, proteinase, biological body membranes and other virulence factors can be significantly reduced, and the effect of the compound in inhibiting colony induction is in the concentration dependence.

The invention discloses a method for increasing the content of flavonoids in hydroponic lettuce in a greenhouse by adding UV-B radiation. The method comprises the steps of taking lettuce seeds, conducting disinfecting and placing the seeds on gauze infiltrated with a 1 / 2 Hoagland nutrient solution; placing the gauze and lettuce seeds in a dish, sealing the dish with a plastic wrap with holes, placing the dish in an environment with a temperature of 15-20 DEG C and white light to conduct cultivation until germination, continuing conducting cultivation for 5-7 days, then conducting hydroponics by a 1 / 2 Hoagland nutrient solution for 2 to 3 weeks, then adding UV-B radiation to the lettuce seedlings for 3 to 4 hours per day, repeating the UV-B radiation operation for 6 to 8 days, and then harvesting lettuce. The method of the invention can significantly improve and stably maintain the content of flavonoids in the hydroponic lettuce in the greenhouse, wherein the flavonoid content increasesby 30% or more after 6 days of UV-B radiation, the nutritional quality of the lettuce is increased, and growth of the lettuce is not inhibited, and the organism amount is not affected.

The invention relates to the technical field of pesticides and discloses a bactericide and a preparing method thereof. The bactericide comprises, by weight, 5-10 parts of solidago canadensis volatile oil, 6-11 parts of validamycin, 3-5 parts of welsh onion, 15-30 parts of licorice, 3-14 parts of purslane, 2-10 parts of Chinese prickly ash, 2-7 parts of bitertanol, 25-41 parts of a solvent, 11-16 parts of a cosolvent and 2-12 parts of an antifreezing agent. The bactericide has advantages of low toxicity, environment protection, a wide bactericidal spectrum, a long duration period and capability of effectively delaying drug resistance and is used for crop disease control in agriculture.

The invention provides an application of pyrimidine compounds in the promotion of metabolite synthesis and the hormone level in rice. The pyrimidine compounds are 6-(methoxymethyl)-2-[5- (trifluoromethyl)-2-pyridyl]pyrimidin-4-ol. The compounds can obviously rapidly improve the pathogen invasion resistance of plants, does not inhibit the growth of the plants or the growth of a root system, and also promotes the synthesis of the metabolites and the increase of the hormone content in the rice.

The invention belongs to methods for killing bacteria and fungi in chlamydomonas reinhardtii and discloses a method for sterilization in a chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid. The method includes: A, inoculating chlamydomonas contaminated by mixed bacteria onto a TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in an occulting light period of 14 / 10 and under the light intensity of 8000Lx; C, transferring the chlamydomonas well grown on the TAP solid medium plate to a TAP plate with mixed antibacterial agents, and culturing for 5-8 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx; D, transferring the sterilized chlamydomonas to the common TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx to achieve a standby for subsequent experiments or breed conservation. The method has the advantage that the problem of bacterial contamination in scientific experiment and application process of chlamydomonas is solved by adoption of the mixed antibacterial agents for treating bacterial and fungus contamination of chlamydomonas.

The present invention discloses an application of flavone oxygen glycoside compounds in preparations of bacterial quorum sensing inhibitory drugs. Structure of the compounds is as described in a formula I; wherein R1 is H, hydroxy or methoxy; R2 is hydroxy; R3 is H, hydroxy or galactoside; and R4 is hydroxy or galactoside. The present invention provides a novel use of the flavone oxygen glycosidein preparations of the bacterial quorum sensing inhibitory drugs. Experimental results show that the flavone oxygen glycoside can all inhibit biofilms of pseudomonas aeruginosa 9027, pseudomonas aeruginosa 27853 and drug resistance pseudomonas aeruginosa, show that the flavone oxygen glycoside compounds can inhibit the bacterial quorum sensing system to reduce bacterial virulence and pathogenicity, while bacterial growth is not inhabited and bacteria are not easy to produce the drug resistance of the bacteria, it is indicated that the flavone oxygen glycoside compounds can be prepared into a pseudomonas aeruginosa quorum sensing inhibitor.

The invention discloses new application of a natural product hordenine and in particular relates to application of a compound hordenine in preparation of a medicine used for preventing and treating bacterial infection. The new application is mainly reflected in that hordenine can obviously inhibit synthesis of a quorum sensing-regulated signal molecule, hordenine can obviously inhibit expression of a quorum sensing-regulated virulence factor and hordenine can obviously inhibit expression of a quorum sensing-related gene. Besides, drug combination of hordenine and antibiotic netilmicin can obviously inhibit generation of a biofilm, a formed biofilm can be obviously destroyed, and the hordenine is hopeful to becoming a novel anti-infective agent for preventing and treating bacterial infection.

The invention discloses N-15 marked microcystis and a production method thereof. The production method comprises the following steps of microcystis aeruginosa cleaning: using the microcystis aeruginosa in a logarithmic phase as microcystis aeruginosa seeds, performing suspension cleaning on the microcystis aeruginosa seeds by deionized water, and collecting microcystis aeruginosa bodies after centrifugation, wherein the number of cleaning times is at least two; microcystis aeruginosa culture: inoculating the cleanly cleaned microcystis aeruginosa into a special culture medium with N-15, performing culture in light (the light intensity is about 2500 to 5000LUX, and the temperature is 25 to 28 DEG C), shaking up a culture bottle for 1 to 5 times every day, and performing culture for 10 to 30days to obtain culture liquid; and microcystis extraction: performing lysis treatment on microcystis aeruginosa cells in the culture liquid, performing centrifugation, and collecting liquid supernatant to obtain microcystis crude extraction liquid. The production method is simple; the operation is easy; the N-15 marking rate of the prepared microcystis is high; when the prepared microcystis is used as a standard product, a matrix effect is reduced; and the quantification is more accurate. Compared with the existing non-standard microcystis, better application prospects are realized.

The invention discloses a pear fruit calyx-eliminating flower-thinning disease-preventing agent as well as an application method and application of the pear fruit calyx-eliminating flower-thinning disease-preventing agent. The calyx-eliminating flower-thinning disease-preventing agent comprises the following components: 15-25mg / L flusilazole and 45-55mg / L 6-benzyl purine. Compared with the prior art, the calyx-eliminating flower-thinning disease-preventing agent can not degrade the fruit quality and inhibit tree growth on the basis that the calyx-eliminating rate of pear fruits is improved, and meanwhile as the flusilazole has the function of fungus control, the calyx-eliminating flower-thinning disease-preventing agent further has the functions of preventing pear tree ring spot, rot, scab and anthracnose.

The invention relates to a microbial agent for preventing and treating the white stool syndrome of litopenaeus vannamei as well as a preparation method and application of the microbial agent. The microbial agent is fermented powder prepared by performing spray drying on fermentation liquid of enterococcus faecalis. The preparation method of the microbial agent for preventing and treating the whitestool syndrome of the litopenaeus vannamei comprises the following steps: sequentially performing shake-flask culture, first-stage seed culture, second-stage seed culture and fermentation culture onthe enterococcus faecalis, and then drying to obtain the microbial agent for preventing and treating the white stool syndrome of the litopenaeus vannamei. In the application of the microbial agent forpreventing and treating the white stool syndrome of the litopenaeus vannamei, the microbial agent is blended into feed in proportion, and then the feed is used for feeding the litopenaeus vannamei ina pond in which white stool is formed for treatment or not formed for prevention. The microbial enterococcus faecalis agent can significantly inhibit growth and reproduction of pathogens such as vibrio cholerae, edwardsiella tarda and aeromonas hydrophila, has broad-spectrum bacteriostasis, can prevent and treat the white stool disease of the litopenaeus vannamei, has the preventing and treatingeffect of 78% or above, and has a significant effect.

The invention belongs to the technical field of medicines, and particularly relates to application of a compound in preparation of a medicine for inhibiting a bacterial quorum sensing system. The invention provides application of a compound in preparation of a medicine for inhibiting a bacterial quorum sensing system. Experiment results show that the 2-(3', 4'-dihydroxyphenyl)-1, 3-piperidine-5-aldehyde can inhibit three signal paths of a Pseudomonas aeruginosa quorum sensing system pqsA, lasB and rhlA and inhibit the activity of the Pseudomonas aeruginosa quorum sensing system without inhibiting the growth of the Pseudomonas aeruginosa. Therefore, the pathogenicity and toxicity of the bacteria are reduced without inhibiting the growth of the bacteria, and the generation of multiple drug resistance of the bacteria is slowed down.

A method for removing bacteria of chlamydomonas reinhardtii through kresoxim-methyl, ampicillin and cefotaxime mixed antibacterial agents comprises the following steps: inoculating mixed and pollutedchlamydomonas through a TAP solid culture medium flat plate, and culturing; preparing a mixed antibacterial agent flat plate which is a TAP solid culture medium in which kresoxim-methyl, ampicillin and cefotaxime are added, and waiting for later use; transferring and marking the mixed and polluted chlamydomonas which is cultured on the TAP solid culture medium to the antibacterial agent mixed TAPsolid culture medium flat plate for culturing; and transferring and marking the antibacterial chlamydomonas from the antibacterial flat plate to a common TAP solid culture medium flat plate to cultureagain. According to the method, kresoxim-methyl, ampicillin and cefotaxime are used in match to effectively remove the bacteria and fungus pollution in the chlamydomonas culture process; the method has the characteristics of being high in wide popularization scope, efficient, and low in toxicity, and is extremely high in value of popularization and application in storing of precious chlamydomonasand accuracy improving of following experiments.

The invention discloses a new medical application of a radix isatidis extract and provides a medicine capable of effectively antagonizing a multidrug-resistant bacterium. The radix isatidis preparation can be used for treating diseases caused by the multidrug-resistant bacterium containing an NDM-1 drug-resistant gene, such as intestinal infection, respiratory infection, wound and skin infections, urogenital infection, bacteremia, meningitis and the like, and is particularly applicable to treatment of various infectious diseases caused by calcium acetate acinetobacter, acinetobacter baumannii, stenotrophomonas maltophilia, or escherichia coli containing the NDM-1 drug-resistant gene.

The invention provides a triterpenoid-saponin compound, wherein the molecular formula is C52H84O21, and the chemical name is 3beta-O-{beta-D- xylopyranosyl-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-[beta-D-glucopyranosyl(1->4)]-alpha-L-arabinopyranosyl}hederagenin. In-vitro anti-tumor tests indicate that the compound has a remarkable inhibiting action for C6 rat glioblastoma cells as well as four kinds of U87MG, U251MG, BT325 and SHG44 glioblastoma cells without influencing the growth of primarily-cultured human neuroglia cells. The compound is hopeful to be used for preparing a medicament for treating a glioma.

The invention relates to a method for preventing and treating anorexia of fish. A composite microbial agent used in the invention comprises bacillus amyloliquefaciens powder and bacillus licheniformispowder, wherein a mass ratio of the bacillus amyloliquefaciens powder to the bacillus licheniformis powder is 1: 1. The powder of the composite microbial agent is put into a pond, and the bacillus amyloliquefaciens powder and bacillus licheniformis powder are matched with each other, so an obvious prevention and treatment effect on anorexia of fish is achieved; and the composite microbial agent has the characteristics of no phytotoxicity, no residues, no growth inhibition and small in dosage, and possibility is provided for diversification of medication schemes and protection of an ecologicalenvironment.

The invention discloses a gynecological disinfectant and a preparation method thereof, and belongs to the field of medical disinfectants. The gynecological disinfectant comprises the following components in percentage by mass: 0%-5% of glycerol, 0%-5% of polyethylene glycol-400, 0%-5% of tween-20, 0%-5% of tween-40, 8%-10% of vitamin C, 0%-2% of vitamin E, 0.4%-2% of folic acid, 0.5%-2% of acetate, 2%-8% of acetic acid and the balance of water. The gynecological disinfectant can be obtained by adding and uniformly mixing the components according to a certain sequence. The gynecological disinfectant has a broad-spectrum bactericidal effect on staphylococcus aureus, escherichia coli, candida albicans and the like which are common in gynecological vaginitis, cannot kill beneficial bacteria such as lactic acid bacteria and the like and can regulate and stabilize pH of vagina, the acting time is long, all the components are non-toxic, harmless and non-irritant, and the gynecological disinfectant has mild effects and is quite suitable for being used as a safe and effective compound disinfectant for treating vaginitis.

The invention relates to the technical field of industrial cultivation of edible fungi. More specifically, the invention discloses a method for sterilizing a liquid culture medium of an edible fungus strain. The method comprises the following steps: in an aseptic environment, mixing slightly acidic electrolyzed water with the liquid culture medium of the edible fungus strain according to a volume ratio of (1-2):20, shaking uniformly, and sealing the mixture to achieve an effect of killing heterogeneous bacteria. In comparison with a conventional high-temperature high-pressure sterilization method, the method disclosed by the invention not only saves water, electricity, and time costs, but also reduces the risk caused by the conventional method. Moreover, in comparison with reported sterilization by using slightly acidic electrolyzed water, the method disclosed by the invention does not inhibit the growth of the edible fungus strain while killing the heterogeneous bacteria, improves the strain inoculation efficiency, and meanwhile, a pungent odor generated by high-concentration active chlorine contained in strong acid water is avoided from causing adverse impact on the health of workshop staffs.