The invention relates to a preparation method of a composite probiotic ice cream. The preparation method comprises the following steps of (1) weighing raw materials according to the formula: whole milk powder, soybean milk powder, water, cane sugar, beta-cyclodextrin, soybean protein powder, inulin, honeysuckle flower powder, Chinese wolfberry fruit powder and liquiritigenin for standby application; and (2) uniformly mixing and stirring the weighed raw materials in step (1), then performing homogenizing, performing sterilization and performing ageing to obtain an ice cream mixture, then adding0.003-0.008 part of composite probiotics to the ice cream mixture, performing uniform stirring to obtain a mixture, sending the mixture to a condensing and freezing machine for condensing and freezing, after condensing and freezing, performing filling, and performing hardening, so as to obtain the probiotic ice cream. The ice cream made by the preparation method is rich in nutrition, various probiotics and traditional Chinese medicine components, and has good health-care functions. According to the method, the ice cream is compounded from specific components in specific parts by weight, so that the survival rate of probiotics for the probiotic ice cream in the preparation and preservation process can be increased, and normal exertion of the health-care functions of the probiotic ice creamcan be guaranteed.