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Bacillus coagulans MES847, microbial inoculum, chicken feed, preparation method and application

A kind of technology of Bacillus coagulans, MES847

Active Publication Date: 2020-04-10
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] When treating diseases, taking several antibiotics at the same time will easily cause microbial imbalance in the intestinal tract of meat (egg) chickens, and harmful microorganisms will multiply, resulting in a significant decrease in feed intake, loose stools, and a decrease in weight gain.
[0011] In addition, the salt content in the feed is too high, the anion and cation of the feed are seriously unbalanced, and the protein in the feed is too high, which will also cause diarrhea in (meat) laying hens.

Method used

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preparation example Construction

[0046] The present invention also provides a preparation method of the above bacterial agent, the preparation method comprising successively undergoing shake flask culture, primary seed cultivation, secondary seed cultivation, fermentation and spray drying of Bacillus coagulans MES847 to obtain the bacterial agent.

[0047] The preparation method of the bacterial agent provided by the present invention has simple process and convenient operation, and is suitable for large-scale expansion and cultivation.

[0048] Specifically, the preparation method of the above bacterial agent comprises the following steps:

[0049] ① Shake flask culture: The strains activated on the slant were subjected to seed shake flask culture, and the shake flask medium was: MRS medium (10g of beef protein powder, 10g of fish juice, 5g of yeast extract juice powder, 20g of glucose, 5g of sodium acetate, citric acid Diammonium 2g, Tween 80 0.1ml, magnesium sulfate 0.58g, manganese sulfate 0.28g, distille...

Embodiment 1

[0063] The isolation and screening of embodiment 1 bacterial strain

[0064] The strain of Bacillus coagulans MES 847 was isolated from the soil in Baoding City, Hebei Province by our R&D personnel in May 2018. At that time, a total of 3 strains of lactic acid bacteria were isolated, streaked on a plate, cultured at 32°C for 36 hours, made into slant strains, and refrigerated at 4°C. A single colony was picked, sliced, stained with crystal violet, examined under a microscope, and physiological and biochemical tests finally confirmed that one of the strains was Bacillus coagulans, and named it MES 847.

Embodiment 2

[0065] The preparation of embodiment 2 bacillus coagulans MES 847 inoculum

[0066] The method comprises: shaking flask culture, primary seed cultivation, secondary seed cultivation, fermentation, spray drying and the like. specifically:

[0067] ① Shake flask culture: The strains activated on the slant were subjected to seed shake flask culture, and the shake flask medium was: MRS medium (10g of beef protein powder, 10g of fish juice, 5g of yeast extract juice powder, 20g of glucose, 5g of sodium acetate, citric acid Diammonium 2g, Tween 80 0.1ml, magnesium sulfate 0.58g, manganese sulfate 0.28g, distilled water 1000ml, adjust the pH value to 6.2-6.6). The culture conditions are: rotation speed 200-230r / min, temperature 32-37°C, culture 30-36h.

[0068] ②Primary seed culture: 100ml of primary seed culture medium is placed in a 500ml eggplant bottle, sterilized by moist heat at 116°C-121°C for 20-35min, and 10ml of live Bacillus coagulans freeze-dried tube strains are inocul...

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Abstract

Relating to the technical field of microorganisms, the invention provides a bacillus coagulans MES847, a microbial inoculum, a chicken feed, a preparation method and application. The bacillus coagulans MES847 provided by the invention is preserved in China General Microbiological Culture Collection Center on August 30, 2018, and the preservation number is CGMCC NO.16358. The bacillus coagulans MES847 has a wide antibacterial spectrum, can be used for preparation of products preventing and / or treating chicken diarrhea, at the same time can improve the cellular immunity and humoral immunity level of organisms, stimulates the development of animal immune organs, and improve the disease resistance of animals.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a preparation method and application of bacillus coagulans MES847, bacterial agent, and chicken feed. Background technique [0002] In recent years, broiler (egg) chickens have more feed droppings and watery stools. The clinical symptoms are: small particles of feed that can be digested by the stomach remain in the feces, and the water content in the feces is high; the growth rate of broilers decreases, and the laying hens lay eggs. Feed rate decreased and feed conversion rate decreased. In the absence of secondary diseases, the mortality rate of poultry is usually not high. This kind of loose stool symptom has a complicated etiology, and there are often no obvious characteristic lesions in anatomical examination, so it is difficult to determine the etiology, which brings certain difficulties to clinical prevention and treatment. [0003] At present, the reasons for the format...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/75A23K10/18A61K35/742A61P1/12A61P1/14C12R1/07
CPCA61K35/742A23K10/18A23K50/75A61P1/12A61P1/14C12N1/20C12N1/205C12R2001/07
Inventor 刘刚孙海远顾欣燕刘金龙肖培英
Owner 天津开发区坤禾生物技术有限公司
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