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Method for sterilizing liquid culture medium of edible fungus strain

A technology of liquid culture medium and sterilization method, which is applied in the field of sterilization of edible fungus strain liquid culture medium, can solve problems such as pungent odor and corrosion equipment, and achieve the effects of reducing risk, avoiding adverse effects, and saving water and electricity

Active Publication Date: 2016-07-13
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with strong acid electrolyzed water (pH2~3), slightly acidic electrolyzed water also has a good bactericidal effect, but it avoids corrosion of equipment and pungent smell caused by too low acidity and high available chlorine concentration (80~150ppm) in strong acid electrolyzed water odor defect

Method used

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  • Method for sterilizing liquid culture medium of edible fungus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The configured Flammulina velutipes strain liquid culture medium is subpackaged in 500ml glass conical flasks according to 200ml / bottle. Draw 10ml of slightly acidic electrolyzed water (pH6.4, ACC20.93ppm) into the above-mentioned Erlenmeyer flasks with a pipette gun in a sterile ultra-clean bench, shake well, seal, and complete the sterilization of Flammulina velutipes liquid culture medium.

[0033] Randomly check the sterilization effect of the above-mentioned sterilized medium: randomly select 5 bottles of the above-mentioned sterilized medium, serially dilute three concentrations, absorb 1ml of each concentration gradient and evenly spread it on the PDA medium, and make three parallels for each gradient , placed in a 26°C incubator for 7 days. The observation results showed that there was no colony growth in the plate.

[0034] Investigate the impact of slightly acidic electrolyzed water on the production of Flammulina velutipes mycelium: Flammulina velutipes bact...

Embodiment 2

[0038] The liquid culture medium of Pleurotus ostreatus strain is divided into 500ml Erlenmeyer flasks according to 200ml / bottle. Draw 15ml of slightly acidic electrolyzed water (pH6.2, ACC18.26ppm) into the above-mentioned Erlenmeyer flasks with a pipette gun in a sterile ultra-clean bench, shake well, insert Pleurotus ostreatus strains, and cultivate them in a constant temperature shaking incubator 7d. After the cultivation is finished, the liquid culture solution of the strain is injected into the solid medium with a pipette gun, and placed in a cultivation room to cultivate fruiting bodies of Pleurotus ostreatus. After random inspection, no bacterial contamination occurred throughout the process.

Embodiment 3

[0040] Black fungus seed liquid culture medium is divided into 500ml Erlenmeyer flasks according to 200ml / bottle. Draw 18ml of slightly acidic electrolyzed water (pH5.5, ACC15ppm) into the above-mentioned Erlenmeyer flasks with a pipette gun in a sterile ultra-clean bench, shake well, insert Pleurotus ostreatus strains, and cultivate them in a constant temperature shaking incubator for 7 days. After the cultivation is finished, the liquid culture solution of the strain is injected into the solid medium with a pipette gun, and placed in a cultivation room to cultivate fruiting bodies of Pleurotus ostreatus. After random inspection, no bacterial contamination occurred throughout the process.

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Abstract

The invention relates to the technical field of industrial cultivation of edible fungi. More specifically, the invention discloses a method for sterilizing a liquid culture medium of an edible fungus strain. The method comprises the following steps: in an aseptic environment, mixing slightly acidic electrolyzed water with the liquid culture medium of the edible fungus strain according to a volume ratio of (1-2):20, shaking uniformly, and sealing the mixture to achieve an effect of killing heterogeneous bacteria. In comparison with a conventional high-temperature high-pressure sterilization method, the method disclosed by the invention not only saves water, electricity, and time costs, but also reduces the risk caused by the conventional method. Moreover, in comparison with reported sterilization by using slightly acidic electrolyzed water, the method disclosed by the invention does not inhibit the growth of the edible fungus strain while killing the heterogeneous bacteria, improves the strain inoculation efficiency, and meanwhile, a pungent odor generated by high-concentration active chlorine contained in strong acid water is avoided from causing adverse impact on the health of workshop staffs.

Description

technical field [0001] The invention relates to the technical field of industrial cultivation of edible fungi. More specifically, the present invention relates to a method for sterilizing edible fungus strain liquid culture medium. Background technique [0002] Because of its delicious taste and rich nutrition, mushrooms are loved by consumers, and the market demand is also showing a rapid upward trend year by year. The cultivation of edible fungi has also been gradually upgraded from traditional family farmer cultivation to large-scale factory cultivation. The industrial cultivation of edible fungi not only significantly increases the production of edible fungi, but also has the characteristics of standardized personnel operation and process standardization, which largely avoids strain contamination and huge economic losses caused by incomplete sterilization. At present, in the cultivation process of edible fungi, high-temperature and high-pressure steam equipment is main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/18
CPCA61L2/18
Inventor 聂莹唐选明丁洋赵金红
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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