The invention relates to an industrialized production method of pleurotus eryngii. The industrialized production method of the pleurotus eryngii comprises the steps of strain cultivation, compost preparation and fungus bag manufacturing, inoculating, fungus germination management, bud trimming management, harvesting and packaging. The industrialized production method of the pleurotus eryngii has the advantages that bagasse is used as one of the main materials for the cultivation of the pleurotus eryngii, the range of cultivation materials of the pleurotus eryngii is widened, using capacity of agriculture waste is enlarged, environment pollution is reduced, extension of the industrial chain of sugarcane production is promoted, and due to the fact that the industrialized production method of the pleurotus eryngii can not be influenced by natural factors easily, the pleurotus eryngii can be produced all year round, efficiency is increased by more than 60% compared with the efficiency by adopting a traditional cultivation method, biological efficiency can reach to 60-80%, fungus bag inoculating efficiency is improved due to the fact that battens coated with parent fungi are adopted for the inoculating of the fungus bag, the success rate of the fungus bag inoculating reaches to more than 99.9%, contamination rate of the fungus bag inoculating is reduced, fungus germination is fast, time for the fungus germination of the fungus bag is shortened, and production efficiency is improved.