174results about How to "Strong pathogenicity" patented technology

Disclosed is a means of controlling plant pests by a novel method of expressing Cry2Ab B. thuringiensis delta-endotoxins in plants, targeted to the plastids. The invention comprises novel nucleic acid segments encoding proteins comprising Cry2Ab B. thuringiensis delta-endotoxins. The nucleic acid segments are disclosed, as are transformation vectors containing the nucleic acid segments, plants transformed with the claimed segments, methods for transforming plants, and methods of controlling plant infestation by pests.

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

The invention relates to a Beaueria bassaria(Balsamo)Vuillemin SCWJ-2 strain and a culture method thereof, a spore powder prepared from the strain and application of the spore powder in preparing a biocontrol preparation. The biocontrol preparation can be used for preventing and treating sweetpotato whitefly, Frankliniella occidentalis, Neotoxoptera formosana and / or peach aphid. The biocontrol preparation can be used for preventing and treating insects in a high-temperature environment, and has favorable preventing and treating effects; and the field control effect is higher than 75%.

The invention relates to a Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3, a culture method of the bacterial strain, spore powder prepared from the bacterial strain and application of the spore powder in preparing a biocontrol agent, wherein the biocontrol agent can be used for preventing and controlling bemisia tabaci, frankliniella occidentalis, green onion aphidand / or green peach aphid. The biocontrol agent can be used for preventing and controlling insects under a high temperature environment, and is good in prevention and control effect with field control efficiency over 75%.

The invention relates to the field of haemophilus parasuis vaccines in veterinary biological products, in particular to a haemophilus parasuis LC strain. The collection number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.5257. The invention also relates to application of the haemophilus parasuis LC strain to preparation of haemophilus parasuis inactivated vaccines. The haemophilus parasuis LC strain has stronger pathogenicity to pigs and has better immunogenicity; an inactivated alumina gel vaccine prepared by the strain is safe and reliable; not only a homologous attacking protection is provided, but also a better cross protection to blood serums type 4, type 5, type 10, type 12, type 14 and type 15 HPS (Hantavirus Pulmonary Syndrome) heterologous attacking can be provided; after the pigs are immunized, a stronger immunity can be generated and the morbidity and the mortality of the inoculated pigs are obviously reduced; the immune effect achieves or is better than the traditional commercialized vaccines in the market; the vaccine has the advantages to compete with like products at home and abroad and is capable of effectively preventing the epidemic and the transmission of a haemophilus parasuis disease and reducing the economic losses caused by the disease, so that the application range is wide.

The invention relates to a Lecanicilliumpsalliotae strain used for preventing and treating diaphorina citri and belongs to the technical field of microorganisms. The Lecanicilliumpsalliotae strain ZJLP09 is registered and preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCCNo.7551. The Lecanicilliumpsalliotae strain ZJLP09 has strong pathogenicity on diaphorina citri; compared with a congeneric verticillum lecanii strain, the Lecanicilliumpsalliotae strain ZJLP09 has a stronger effect on the diaphorina citri and can be developed into a biological fungi-proofing preparation used for preventing and treating the diaphorina citri; and compared with the existing chemical agent used for preventing and treating the diaphorina citri, the Lecanicilliumpsalliotae strain ZJLP09 has the characteristics of high efficiency, low toxicity, low residue, no pollution and difficulty in formation of drug resistance and has a good market application prospect.

The invention discloses a Beauveria bassiana strain having high pathogenicity to silkworms and an application of the Beauveria bassiana strain in the production of Bombyx Batryticatus. The strain is preserved at the China General Microbiological Culture Collection Center on February 24, 2014 and the preservation number is CGMCC NO. 8861. The method for producing Bombyx Batryticatus by using the strain disclosed by the invention comprises the steps of preparing a spore suspension by using Beauveria bassiana and sterile water containing 0.2% of Tween-80, uniformly spraying the spore suspension onto the fifth-instar silkworm and inoculating for 4-5 days and obtaining Bombyx Batryticatus after a large quantity of silkworm died from muscardine. The Beauveria bassiana strain disclosed by the invention has high pathogenicity to the silkworm, the death time of the silkworms is consistent, the rigescence rate is high and is conducive to solving the problems about the standard production, quality control of Bombyx Batryticatus and the like.

The invention provides a curvularia lunata strain and application thereof. The strain is curvularia lunata B6 of which the preservation number is CGMCC No.2928. The curvularia lunata strain B6 CGMCC No.2928 has strong and fast pathogenicity to echinochloa crusgalli; field experiments show that the echinochloa crusgalli shows spot and dead symptoms in a week after spores of the curvularia lunata strain are sprinkled to the echinochloa crusgalli and the fresh weight inhibition rate to the echinochloa crusgalli can reach 90 percent in 28 days. The fungus strain curvularia lunata strain B6 CGMCC No.2928 is separated from echinochloa crusgallis naturally having diseases in fields without any artificial gene modification; therefore, the curvularia lunata strain cannot have any ecological and environmental hidden danger when released to nature and is an absolutely-safe biological prevention and control medium.

The invention relates to a biological fermentation feed and a preparation method thereof, belonging to the field of solid feeds. The biological fermentation feed provided by the invention is prepared from a compound bacteria solution and a fermentation substrate through fermentation, wherein the compound bacteria solution comprises bacillus subtilis, composite saccharomycetes, lactic acid bacillus and bifidobacterium species; the fermentation substrate comprises the following components in parts by weight: 30-35 parts of corns, 10-15 parts of soya bean meal, 25-35 parts of rice washing bran, 10-15 parts of wheat bran, 3-8 parts of yeast extract powder, 0.5-2 parts of sodium chloride, 1-5 parts of calcium hydrophosphate, 0-3 parts of soybean oil, 0.5-1.5 parts of manganese sulfate and 0.5-1.5 parts of magnesium sulfate. The biological fermentation feed provided by the invention is free from antibiotics, is green and safe, is prepared from the raw materials with wide sources, is large in maneuverability, has remarkable benefits, and is capable of reducing morbidity of various diseases.

The invention aims to provide haemophilus parasuis and application thereof. The preservation number of haemophilus parasuis is CGMCC No. 10230. The haemophilus parasuis is used for preparing medicines for treating haemophilus parasuis. A haemophilus parasuis serum type 5 vaccine strain LX-5 has a stable biological characteristic, has relatively strong pathogenicity to a piglet, and has good immunogenicity. A propolis inactivated vaccine prepared by applying the haemophilus parasuis is safe and reliable and can generate an antibody in a relatively high level, is long in duration and has a good protective effect on the piglet counteracting toxic substances for homologous strains. The morbidity and the death rate of the immunized swinery are obviously reduced, and the immune effect of the vaccine reaches or is superior to existing commercialized vaccines in the market, so that prevalence and spread of the haemophilus parasuis can be effectively prevented, the economical loss caused by the disease is lowered, and the haemophilus parasuis has a broad application prospect.

The prevention and treatment of influenza is described, by using passive immunization with antibodies (e.g. polyclonal, monoclonal, etc.) to one or more conserved influenza proteins. Both antibodies and fragments thereof are contemplated, raised against conserved proteins such as nucleoproteins.

The invention relates to a porcine epidemic diarrhea virus strain MY01 and application thereof and belongs to the field of porcine epidemic diarrhea virus vaccine reagents. The porcine epidemic diarrhea virus strain MY01 is collected in China General Microbiological Culture Collection Center under CGMCC No. 11495. The porcine epidemic diarrhea virus strain MY01 is higher in pathogenicity for pigs and good in immunogenicity, a deactivated oil emulsion vaccine prepared using the strain is safe and reliable, homologous attacking protection can be provided, protection is also provided for PEDV (porcine epidemic diarrhea virus) epidemic strains, higher immunity can be generated after immunization, inoculated pigs are significantly lower in morbidity and mortality, the immunization effect is superior than that of existing commercial vaccines in the market, and the strain has the advantage of competing with like products home and abroad, can effectively prevent the prevalence and spread of porcine epidemic diarrhea virus and reduce economic cost caused by the disease and has a promising application prospect.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses a serotype 5 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is XX0306. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2013095 and collection date being March 21, 2013. The serotype 5 HPs strain XX0306 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention provides metarhizium rileyi MRJCBT190808 and application thereof to control of spodoptera frugiperda. The metarhizium rileyi MRJCBT190808 is preserved in the China General Microbiological Culture Collection Center on August 21, 2019, the preservation number is CGMCC No.18122, and the preservation address is No.3, No.1 courtyard, Beichen west road, Chaoyang district, Beijing. The metarhizium rileyi MRJCBT190808 is obtained by the processes that wild metarhizium rileyi strains are separated from collected larva bodies of infected spodoptera frugiperda, and cultured on a sacharovi medium, conidia are transferred to a new medium for purification, and then single spore isolation is conducted to obtain the purified metarhizium rileyi MRJCBT190808. The provided metarhizium rileyi MRJCBT190808 is biocontrol fungi capable of being used for control of the spodoptera frugiperda, the characteristics of high pathogenicity to larvae of the spodoptera frugiperda, environmental protection and no pollution, and difficult drug resistance production are achieved, and the metarhizium rileyi MRJCBT190808 can be widely used for biological control of the spodoptera frugiperda.

The invention provides metarhizium anisopliae MAYX130921 and an application thereof. The metarhizium anisopliae MAYX130921 is preserved on Dec 4th, 2014 in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.10139. The metarhizium anisopliae MAYX130921 is a bacterial strain initially separated from holotrichia parallela larvas, is easy to culture and can grow rapidly, sporulation quantity is high, and spore germination rate is high; meanwhile, conidiospores of the metarhizium anisopliae MAYX130921 have strong pathogenicity on the holotrichia parallela larvas, are environment-friendly and hardly produce drug resistance and can be widely applied to prevention and control of holotrichia parallela.

The invention relates to a serratia marcescens strain separated from phyllotreta striolata fabricius and applications thereof. A serratia marcescens strain is directly separated from phyllotreta striolata fabricius larva for the first time, and was preserved in China General Microbiological Culture Collection Center (CGMCC) in July 22th, 2013, with the preservation number of CGMCC No.7948. The serratia marcescens PS-1 strain has strong pathogenicity on the phyllotreta striolata fabricius larva and imagoes and laphygma exigua larva and imagoes, and can effectively control the development of laphygma exigua experiment populations. The serratia marcescens strain can provide technological base for the control on the field populations of phyllotreta striolata fabricius and laphygma exigua, and has important practical significance and practical values on safely, effectively and sustainably controlling the phyllotreta striolata fabricius and laphygma exigua and on efficiently ensuring the output and quality of vegetables.

The invention discloses a Mycoplasma bovis and application thereof. The Mycoplasma bovis is Mycoplasma bovis HNMB1 and is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC NO:13295, the preservation data of November 28th, 2016 and the preservation address of No.3, Courtyard 1, Beichen West Road, Chaoyang District of Beijing. The strain HNMB1 is separated from a diary calf dead due to occurrence of typical respiratory symptoms, no other bacteria grow during separation on a PPLO solid culture medium, only mycoplasmas grow, and proliferation titer in a PPLO liquid culture medium is high and reaches up to more than 10<9>CFU / mL. The strain HNMB1 disclosed by the invention has stronger pathogenicity on the calf, results in pathogenesis and death of the calf and has good immunogenicity.

The invention discloses a beauveria bassiana strain having pathogenicity on cyclophragma undans and an application thereof. The beauveria bassiana strain is named as beauveria bassiana FJBb201202 and preserved in a common microorganism center of China committee for culture collection of microorganisms on March 19, 2015, and the preservation number is CGMCC No. 10641. The strain is high in growth rate, high in spore producing capacity and good in production property; meanwhile, the pathogenicity on the cyclophragma undans is high, the larva death rate reaches 100 percent 15 days after spore suspension with the concentration 107 spores / mL is inoculated into third instar larvae of the cyclophragma undans, and LT50 (the median lethal time) is 5.83. A fungal preparation produced by the strain has the good control effect on the cyclophragma undans and can effectively decrease the forest pest density of the cyclophragma undans; the strain is a biocontrol strain which has the application value on controlling of the cyclophragma undans and has the positive significance for reducing the harm of the cyclophragma undans.

The use of an interferon (IFN) for the manufacture of a medicament for treatment and / or prevention of influenza, preferably avian influenza, is described in the present invention.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses a serotype 4 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is FS0307. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2013094 and collection date being March 21, 2013. The serotype 4 HPs strain FS0307 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention relates to a serum-5 type haemophilus lus paradis vaccine strain. The classified name of the vaccine strain is haemophilus lus paradis, the strain name is JSYZ10 and the vaccine strain is preserved in the China Center for Type Culture Collection on June 15, 2014 with the preservation number of CCTCC NO:M 2014260. The invention further relates to an application of the serum-5 type haemophilus lus paradis vaccine strain in preparation of a haemophilus lus paradis inactivated vaccine. The serum-5 type haemophilus lus paradis JSYZ10 strain is stable in biology, has a strong pathogenicity to a piglet, and has a good immunogenicity when being inactivated and vaccinated on the piglet. A univalent vaccine prepared from the vaccine strain serving as a vaccine candidate strain has good safety, can produce a relatively high antibody on the piglet, has long duration and good immune potency, and can be used for resisting attack of homotype wild strains. After a pig group is immunized, the morbidity and death rate are remarkably reduced, the economic loss of a piggery is reduced, and the immunizing effect of the vaccine strain is the same as or superior to that of existing commercial vaccines on the market.

The invention provides a blood serum 5 type haemophilus parasuis strain JX1002. The blood serum 5 type haemophilus parasuis strain JX1002 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC M 2014127. The strain provided by the invention is stable in biological performance, has very strong pathogenicity to piglets, and has very good immunogenicity when the strain is inoculated to the piglets after inactivation. A monovalent vaccine prepared by using the strain as a vaccine candidate strain is good in safety, can be used for generating relatively high antibodies to the piglets, is long in duration, can be used for resisting the attack of wild strains with the same type, and has very good immune efficiency. An inactivated vaccine can be used for effectively reducing the occurrence of diseases and reducing the economic loss of a pig farm in clinical use, and the immune effect of the inactivated vaccine can reach or exceed that of existing commercial vaccines in markets.

The invention discloses a metarhizium anisopliae strain with strong pathogenicity to curculio nucum and applications of the metarhizium anisopliae strain. The metarhizium anisopliae strain provided by the invention is Metarhizium anisopliae CoCn01, is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 12, 2016 through a patent preservation method, and is assigned with the accession number of CGMCC No.12167. The strain is high in growth speed and strong in spore production capacity, and is separated from the adult corpse of curculio nucum suffering from natural death for the first time, a culture method is simple, the propagation speed is high, the metarhizium anisopliae strain has relatively strong pathogenicity to both the adult and larva curculio nucum, is harmless to human beings and the livestock, and is free of pollution to the environment, the pests have difficulty in generating drug resistance, and thus the metarhizium anisopliae strain can be widely used for preventing and treating curculio nucum, and belongs to a biological control strain which has an ultrahigh application value in the prevention and treatment for curculio nucum.

The invention relates to a tick-killing biological control agent. Suspension emulsion or powder can be prepared from 90 to 99 mass percent of spore powder of beauveria bassiana and 1 to 10 mass percent of celangulin which serve as main raw materials. The spore powder of beauveria bassiana is obtained by performing vacuum drying and pulverization on conidia which is obtained by performing liquid-solid two-phase fermentation on the beauveria bassiana, and vibrating the pulverized conidia by using a 120 to 200-mesh sieve, wherein the spore content is counted by using a blood counting chamber. The tick-killing biological control agent can be prepared into various required formulations such as the powder or the suspension emulsion and the like. The tick-killing biological control agent has the advantages of strong pathogenicity to ticks, safety for humans, domestic animals and other living beings, no environmental pollution, difficult generation of medicament resistance, better comprehensive prevention and control effect, high compatibility between the beauveria bassiana and the celangulin in the compounded biological tick-killing agent, better effect of killing the ticks compared with singly used beauveria bassiana or celangulin, lower cost, simpler production process and easy operation.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses application of a serotype 5 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is XX0306. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC No:M2013095 and collection date being March 21, 2013. The serotype 5 HPs strain XX0306 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention relates to a peanut sclerotium blight greenhouse seedling inoculation method. The peanut sclerotium blight greenhouse seedling inoculation method is characterized by comprising the following steps: 1) obtaining a sclerotium blight strain, namely collecting sclerotium blight sclerotia from the sclerotium blight strain in the field in Wuhan, separating and purifying indoors, carrying out sequence determination and comparison, and identifying Sclerotium rolfsii; 2) preparing inocula carrying bacteria, namely preparing a sclerotium blight bacterium dish, preparing a toothpick carrying the bacteria, and preparing oat granules carrying the bacteria; 3) inoculating the inocula carrying the bacteria to peanut seedlings in different ways; 4) inoculating different quantities of oat granules carrying the bacteria to the peanut seedlings; 5) inoculating the oat granules carrying the bacteria to the peanut seedlings in different seedling ages; and 6) inoculating the oat granules carrying the bacteria to different peanut varieties / lines. The invention establishes a simple and reliable sclerotium blight bacterium inoculation method and is convenient for seedling screening of peanut germplasm resistant to sclerotium blight, a material resistant to the sclerotium blight is obtained, and a technical means is provided for disease resistance breeding.

The invention discloses a Streptococcus suis and application thereof. The Streptococcus suis is Streptococcus suis HNSS1 and is preserved in China Generall Microbiological Culture Collection Center with the preservation number of CGMCC NO:13334, the preservation data of November 22th, 2016 and the preservation address of No.3, Courtyard 1, Beichen West Road, Chaoyang District of Beijing. The strain HNSS1 is separated from a brain tissue of a nursery pig dead due to occurrence of typical respiratory symptoms and neurologic symptoms, no other bacteria grow during separation on a TSA solid culture medium, only streptococcus suis grows, and proliferation titer in a TSB liquid culture medium is high and reaches up to more than 10<9>CFU / mL. The strain HNSS1 disclosed by the invention has stronger pathogenicity on the nursery pig, results in pathogenesis and death of the nursery pig and has good immunogenicity.

The invention discloses a metarhizium anisopliae plant for controlling curculio chinensis chevrolat larvae and application of the metarhizium anisopliae plant. The metarhizium anisopliae plant provided by the invention is named as FJMa 201101, and is collected in China General Microbiological Culture Collection Center on 27th, October, 2014, with a collection number of CGMCC No.9780. The metarhizium anisopliae plant is high in growth speed and strong in sporulation capacity, and has the high pathogenicity on curculio chinensis chevrolat larvae, and when the curculio chinensis chevrolat larvae are inoculated by using a spore suspension of 10<7>spores / ml, the LT50 (lethal time of 50%) of the curculio chinensis chevrolat larvae is 1.65 days. A fungal preparation produced by using the plant has a good control effect on the curculio chinensis chevrolat larvae, is capable of effectively controlling the population density of the curculio chinensis chevrolat larvae, is a biocontrol strain with a potential value on the aspect of controlling the curculio chinensis chevrolat, and has the positive meaning in promotion of high quality and high yield of oil-tea camellia.

The invention discloses an Alterneria tenuissima strain and application thereof in biological weeding. The classified name of the strain is Alterneria tenuissima, the complete name of the strain is Alterneria tenuissima HZ-1, the strain is preserved in the China General Microbiological Culture Collection Center located at the No.3 room, No.1 yard, Beichen west road, Chaoyang district, Beijing city, China on January 11, 2016, and the preservation number is CGMCC No.11992. The Alterneria tenuissima is used for biological weeding of broad-leaved weeds such as galium tricorne, chenopodium album and malva verticillata in a highland barley field, a broad bean field and a pea field and can effectively control and prevent the broad-leaved weeds.

The invention relates to the technical field of prevention and treatment of animal diseases, in particular to a D-type toxigenic pasturella multocida vaccine strain for porcine atrophic rhinitis and an application of the strain. The strain is pasturella multocida TK-MD8 and collected in CGMCC (China General Microbiological Culture Collection Center) in the collection No. CGMCC No.10394 on Jan.27th, 2015. A univalent vaccine prepared from the D-type toxigenic pasturella multocida vaccine strain is simple to produce, safe and efficient.