31results about How to "Good production traits" patented technology

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

The invention relates to a seed producing method for obtaining the hybrid advantage of a crassostrea gigas, which is characterized by comprising the following steps: selecting Chinese and Korean crassostrea gigases as parent oysters to carry out temporary culture; ripening the Chinese and Korean crassostrea gigases synchronously; then hybridizing sperms and ovums of the Chinese and Korean crassostrea gigases by artificial insemination; and finally carrying out the incubation, the seed culture and the growth of the hybridized oyster. The invention cultures a crassostrea gigas hybridized seed with high disease resistance and survival rate and rapid growing speed by hybridizing two crassostrea gigas geographic populations with reproductive isolation; the production performance of the crassostrea gigas hybridized seed is obviously better than that of a Chinese locally inbred comparison population; and the method is simple and has favorable industrialized application prospect.

The invention relates to a Metarhiziumanisopliaevar. anisopliae strain CQMa421 of which the CGMCC (China General Microbiological Culture Collection Center) NO is 4609 and an application thereof in the control of rice leaf roller. The production traits of the strain are good and can grow fast and have high spore-producing capability; and the sporulation rate is up to 6.8%, and the strain has high toxicity. The pesticidal fungal agent produced by using the strain can be used to effectively control the number of the rice leaf rollers.

The invention relates to method of crossbreeding the exotic fresh water crayfish, especially to method of crossbreeding the Luo shi swamp shrimp. The invention introduces the distant Luo shi swamp shrimp sprout A, mating the female parent shrimp and male parent shrimp of the distant Luo shi swamp shrimp sprout A with that of native breed and getting the cross cut B1+ and negative cross B1-; mating the cross cut B1+ and negative cross B1- with the native breed and getting the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-; breeding the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-. The survival rate and seed-accumulating rate of the four kinks of sprout is all good, the composition of water, coarse ash content, coarse protein, coarse fat content, amino acid and aliphatic acid is higher than that of native obviously by applying the technique in this invention, especially the breed-accumulating rate of the cross B2-+ of B1- is increased to 67.25%, the rate of emergence to 94.56%.

The invention discloses a beauveria bassiana DSXJ-07, which has been preserved with the accession number being CGMCC No.7192. The sequence of an internal trasicribed spacer of the beauveria bassiana DSXJ-07 is represented as the Seq ID No.7192. The invention also provides the application of the strain in prevention and treatment on pests on graminaceous crops, wherein the application is used for preparing a drug for preventing and treating the pests on graminaceous crops. The graminaceous crops include paddy rice, corn, sugarcanes, wheat, barley or forage grass. The pests comprise lissorhoptrus oryzophilus kuschel and asiatic corn borer. A pest killing fungus preparation prepared from the strain can effectively control the population quantity of the lissorhoptrus oryzophilus kuschel and asiatic corn borer.

The breeding method of tangerine shell colour Haliotis discus hannai Ino includes the following steps: using the mutant of it, planting it in sea-water, adding natural food, applying natural air to make its sex gland be developed and matured, adopting the processes of drying in the shade, heating and irradiating sea-water by UV-ray to make stimutation to hasten parturition to obtain female gameteand male gamete, using single cross or group cross mode to make oritificial insemination, and utilizing conventional hatching and post-cultivation method so as to obtain the invented new variety.

The intraspecific crossing process of Haliotis asinina of different geographic colonies includes the procedures of seed selection, promoting mature and induced spawning, insemination, hatching and cultivating. The process is simple and easy to operate, and can obtain Haliotis asinine fry with the excellent characters of its parents. The present invention is suitable for industrial production.

The invention discloses a metarhizium anisopliae plant for controlling curculio chinensis chevrolat larvae and application of the metarhizium anisopliae plant. The metarhizium anisopliae plant provided by the invention is named as FJMa 201101, and is collected in China General Microbiological Culture Collection Center on 27th, October, 2014, with a collection number of CGMCC No.9780. The metarhizium anisopliae plant is high in growth speed and strong in sporulation capacity, and has the high pathogenicity on curculio chinensis chevrolat larvae, and when the curculio chinensis chevrolat larvae are inoculated by using a spore suspension of 10<7>spores / ml, the LT50 (lethal time of 50%) of the curculio chinensis chevrolat larvae is 1.65 days. A fungal preparation produced by using the plant has a good control effect on the curculio chinensis chevrolat larvae, is capable of effectively controlling the population density of the curculio chinensis chevrolat larvae, is a biocontrol strain with a potential value on the aspect of controlling the curculio chinensis chevrolat, and has the positive meaning in promotion of high quality and high yield of oil-tea camellia.

The invention provides a metarhizium flavoviride bacterial strain (Metarhizium flavoviride.minus) CQMf1072 with the preservation number of CGMCCNO.4717 and the ITS1-5.8S-ITS2 sequence of SEQ ID NO.1. The metarhizium flavoviride bacterial strain CQMf1072 which is directly separated and screened from naturally infected rice planthopper has strong sporulation capability, and the sporulation rate can be up to 2.5%; the metarhizium flavoviride bacterial strain has good production trait and is rapid to grow; meanwhile, the metarhizium flavoviride bacterial strain has high insecticidal activity on the rice planthopper and has a good insecticidal effect; the LT50 (lethal time) and LT90 (lethal time) of the larva of the rice planthopper are respectively 5.8 days and 8.7 days. By using an insecticidal fungal preparation which is manufactured by utilizing the bacterial strain, the population quantity of the rice planthopper is effectively controlled.

The invention discloses a Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of the Metarhizium anisopliae strain, and belongs to the technical field of biological control. The strain is named Metarhizium anisopliae FJMa201516, and the preservation number is CGMCC No.18123. The strain is high in growth speed and high in spore production capacity, and meanwhile has high pathogenicity to the larvae of the Milionia basalis; after the larvae of the Milionia basalis are inoculated with a 107 spore / mL spore suspension for 15 days, the cumulative deathrate of the larvae reaches 96.7%, and LT50 is 5.01 days; a fungal preparation produced by using the strain has a good control effect on the Milionia basalis, and the population density of the larvaeof the Milionia basalis can be effectively reduced. The strain has potential value in control of the Milionia basalis, and has positive significance in reducing harm caused by the Milionia basalis andreducing the use amount of chemical pesticides.

Provided is cladosporium cladosporioides CQBBW3. The preservation number is CGMCC No.11439. The homology between the rDNA-ITS sequence of the cladosporium cladosporioides and the corresponding sequence of the cladosporium cladosporioides reaches up to 95%. The cladosporium cladosporioides CQBBW3 which is directly separated and screened from naturally-infected citrus red mites is high in conidium-producing ability, the conidium-producing quantity reaches 4.3*10<8> conidia / mL on the fourteenth day, the production character is good, growth is fast, and the colony diameter is 6.6125 cm on the fourteenth day. Meanwhile, the citrus red mite killing activity is high, the citrus red mite killing effect is good, LC50 of female adult mites of citrus red mites is 4.66*10<8> conidia / mL, and LT50 of the female adult mites of the citrus red mites is 6.2353 days. An insecticidal fungus preparation produced through the cladosporium cladosporioides effectively controls the colony quantity of the citrus red mites.

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

The invention discloses a Beauveria bassiana DSXJ-07, which has been preserved, and the storage number is CGMCC No.7192, and the sequence of the internal transcriptional spacer is SEQ ID No.7192. The present invention also provides the application of the bacterial strain for preventing and controlling pests on gramineous crops, and as the application of preparing a medicine for preventing and controlling pests on gramineous crops, wherein the gramineous crops are rice, corn, sugarcane, wheat, barley or forage grass. The pests mentioned are rice water weevil and Asian corn borer. The insecticidal fungal preparation produced by the strain can effectively control the populations of the rice water weevil and the Asian corn borer.

The invention relates to a Nomuraea rileyi strain CQNr126, with a preserving number of CGMCC No.4610. The strain provided by the present invention has a strong sporulation capability. On a 3 / 4 SMAY medium plate, the amount of spores reaches 2.2*10<8> spore / cm<3>. The strains have a good productive performance, grow fast, and have a strong insecticidal activity and a good insecticidal effect against rice leaf rollers and chilo suppressalis. Under the effects of the strains, the median lethal time (LT50) of rice leaf rollers and chilo suppressalis are respectively 6.03 days and 6.42 days. With a fungal preparation prepared from the strains, rice leaf roller population and chilo suppressalis population can be effectively controlled.

The invention relates to method of crossbreeding the exotic fresh water crayfish, especially to method of crossbreeding the Luo shi swamp shrimp. The invention introduces the distant Luo shi swamp shrimp sprout A, mating the female parent shrimp and male parent shrimp of the distant Luo shi swamp shrimp sprout A with that of native breed and getting the cross cut B1+ and negative cross B1-; mating the cross cut B1+ and negative cross B1- with the native breed and getting the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-; breeding the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-. The survival rate and seed-accumulating rate of the four kinks of sprout is all good, the composition of water, coarse ash content, coarse protein, coarse fat content, amino acid and aliphatic acid is higher than that of native obviously by applying the technique in this invention, especially the breed-accumulating rate of the cross B2-+ of B1- is increased to 67.25%, the rate of emergence to 94.56%.

The invention discloses a gene knockout type baculovirus expression vector, and relates to the technical field of biology. According to the gene knockout type baculovirus expression vector, five continuous non-essential genes Ac29, Ac30, Ac31, Ac32 and Ac33 on the baculovirus expression vector are knocked out at the same time, and then the gene knockout type baculovirus expression vector is obtained. According to the gene knockout type baculovirus expression vector disclosed by the invention, the expression level of foreign proteins is improved by 80%. Meanwhile, the proliferation characteristic of the virus is not influenced. And the production cost of enterprises can be obviously reduced by improving the expression yield. The baculovirus expression vector can be used in the industrial field of biological products, especially in the industrial field of subunit vaccines.

The invention discloses a gene knockout type baculovirus expression vector, and relates to the technical field of biology. The gene knockout type baculovirus expression vector is obtained after two adjacent non-essential genes Ac63 and Ac64 on the baculovirus expression vector are knocked out at the same time. According to the gene knockout type baculovirus expression vector disclosed by the invention, the expression level of foreign protein is improved by at least one time, and meanwhile, the proliferation characteristic of the virus is basically not influenced. And the production cost of enterprises can be obviously reduced by improving the expression yield. The baculovirus expression vector can be used in the industrial field of biological products, especially in the industrial field of subunit vaccines.

The invention discloses a rhabdovirus expression vector capable of relieving high-titer inhibition. A rhabdovirus expression system does not have a significant dose effect, that is, the high virus titer cannot increase the yield of exogenous genes, because the high titer induces aggregation of later-period expression factors LEF-10, and then expression of later-period genes is inhibited. Accordingto the vector, through mutation of the 21st leucine site of the LEF-10, aggregation tendency of the LEF-10 is lowered. Insect cells are infected by high-titer mutation rhabdovirus, thus the expression amount, controlled by later-period promoters, of the exogenous genes can be significantly increased, and through increase of the expression yield, the production cost of enterprises can be significantly lowered. The rhabdovirus expression vector can be used in the field of biological product industry, especially the field of subunit vaccine industry.

The invention belongs to the field of microorganisms, and in particular relates to a strain of Curvosporium intensis and its application. The Tolypocladium inflatum strain SCKDC10 of the present invention has a deposit number of CCTCC M2018701, and the sequence homology between its rDNA-ITS sequence and Tolypocladium inflatum isolate is as high as 99%. The present invention has the SCKDC10 strain isolated from the larvae of the bat moth, which has good production characteristics and rapid growth, and the diameter of the colony after cultivating for 15 days can reach 10-12 cm; The insect effect is good, when the spore concentration is 2.0 × 10 8 When spores / mL, the cumulative mortality rate of silkworm larvae was 78.33% on the 8th day, and the cumulative mortality rate of Panonychus citrus mite was 59.5%. The insecticidal fungal preparation produced by this strain effectively controlled a variety of pests. total group number.

The invention relates to the technical field of microorganisms, in particular to a culture method of a high-infectivity cordyceps sinensis strain and application thereof. The invention provides a cordyceps sinensis strain culture and infection method for improving infectivity of cordyceps sinensis to hepialidae larvae. The strain cultured by the method is good in growth character and high in hypha and conidium production capacity. Meanwhile, the infectivity to the hepialidae larvae is high, a bacterial suspension obtained through the method is used for infecting the hepialidae larvae, and the infectivity can reach 86.67% and is obviously higher than that of cordyceps sinensis strains obtained in the nature and through an existing method.

The invention discloses a strain of Metarhizium anisopliae with high pathogenicity to larvae of the blue-footed moth and its application, belonging to the technical field of biological control. The strain was named Metarhizium anisopliae ( Metarhizium anisopliae ) FJMa201516, the deposit number is CGMCC No.18123. The strain has fast growth rate and strong spore-producing ability, and has high pathogenicity to the larvae of the blue-footed moth, with 10 7 15 days after the spore suspension was inoculated with spores / mL larvae, the cumulative mortality rate of the larvae reached 96.7%, LT 50 It is 5.01 days; the fungal preparation produced by the strain has a good control effect on the orange-banded moth, and can effectively reduce the population density of the orange-banded moth larvae. The strain has potential value in the control of L. orange-banded moth, and has positive significance for reducing the harm caused by L. orange-banded moth and reducing the use of chemical pesticides.

An hrf1 gene of rice Xanthomonas is disclosed. It can be used as target gene to be constructed on the Ti-plasmid dual-element vector pBI121 expressed in plant, so obtaining its recombinant vector pBI121:hrf1. Said recombinant vector is transferred to EHA105 to transform plant cells. The transformed regenerative plant (To generation) is screened in the culture medium and detected. The resultant transgenic plant has the improved broad-spectrum power to resist diseases and pests and the improved other productive characteristics.

Provided is acremonium implicatum CQBBW8. The preservation number is CGMCC No.11440. The sequence homology between the rDNA-ITS sequence of the acremonium implicatum and the isolate LR2 of the acremonium implicatum reaches up to 98%. The acremonium implicatum CQBBW8 which is separated from citrus red mite bodies is good in production character and fast in growth, and the colony diameter can reach 5.083 cm after culture is performed for 15 days. Meanwhile, the killing activity on various pests is high, the pest killing effect is good, and the death rate of third instar larva of papilio xuthus linnaeus reaches up to 100%, the death rate of aphids reaches up to 100%, and the death rate of citrus red mites reaches up to 100% when the acremonium implicatum with 10<6> conidia / mL is used for killing the pests. An insecticidal fungus preparation produced through the acremonium implicatum effectively controls the colony quantity of various pests.

The invention belongs to the field of microorganisms, in particular to a Curvularia dilatata strain and an application thereof. The strain SCKDC10 (CCTCC M2018701) of Tolypocladium inflatum of the present invention has homology between a rDNA-ITS and Tolypocladium inflatum isolate as high as 99%. The strain SCKDC10 of Curvularia dilatata isolated from the larvae of the bat moth has good productioncharacteristics and rapid growth, and the diameter of the colony after 15 days of cultivation can reach 10-12cm; and has strong insecticidal activity against many kinds of pests at the same time. Thecumulative mortality to larvae of Bombyx mori and Panonychus citri is 78.33% and 59.5% respectively at the concentration of 2.0 * 108 spores / mL on the eighth day. The insecticidal fungicides producedby this strain could effectively control the number of many kinds of insect pests.

The invention discloses a metarhizium strain pathogenic to Laos false blister beetles and application of the metarhizium strain. The metarhizium strain is named as metarhizium anisopliae [FJMa200902, which is stored in China general microbiological culture collection center (CGMCC)], and the collection number is CGMCC No.10642. The strain grows fast and has strong spore formation capability, good production traits and high pathogenicity to larvae of the Laos false blister beetles; after the strain is inoculated by a spore suspension liquid of 107 spores / mL, LT50 (lethal time of 50%) of the strain to the larvae of the Laos false blister beetles is 3.6 days, and infection rate reaches 100%. A fungus preparation made from the strain has good prevention and treatment effects on the Laos false blister beetles, population density of the larvae of the Laos false blister beetles can be reduced effectively, and the metarhizium strain is a biocontrol strain which has application value for prevention and treatment of the Laos false blister beetles.

The invention provides a metarhizium flavoviride bacterial strain (Metarhizium flavoviride.minus) CQMf1072 with the preservation number of CGMCC NO.4717 and the ITS1-5.8S-ITS2 sequence of SEQ ID NO.1. The metarhizium flavoviride bacterial strain CQMf1072 which is directly separated and screened from naturally infected rice planthopper has strong sporulation capability, and the sporulation rate can be up to 2.5%; the metarhizium flavoviride bacterial strain has good production trait and is rapid to grow; meanwhile, the metarhizium flavoviride bacterial strain has high insecticidal activity on the rice planthopper and has a good insecticidal effect; the LT50 (lethal time) and LT90 (lethal time) of the larva of the rice planthopper are respectively 5.8 days and 8.7 days. By using an insecticidal fungal preparation which is manufactured by utilizing the bacterial strain, the population quantity of the rice planthopper is effectively controlled.

The breeding method of tangerine shell colour Haliotis discus hannai Ino includes the following steps: using the mutant of it, planting it in sea-water, adding natural food, applying natural air to make its sex gland be developed and matured, adopting the processes of drying in the shade, heating and irradiating sea-water by UV-ray to make stimutation to hasten parturition to obtain female gamete and male gamete, using single cross or group cross mode to make oritificial insemination, and utilizing conventional hatching and post-cultivation method so as to obtain the invented new variety.

The invention relates to a Metarhiziumanisopliaevar. anisopliae strain CQMa421 of which the CGMCC (China General Microbiological Culture Collection Center) NO is 4609 and an application thereof in the control of rice leaf roller. The production traits of the strain are good and can grow fast and have high spore-producing capability; and the sporulation rate is up to 6.8%, and the strain has high toxicity. The pesticidal fungal agent produced by using the strain can be used to effectively control the number of the rice leaf rollers.