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Rice flavamonocell hrf3 gene, recombinant vector and breeding method for plant transgene

A xanthomonas, gene technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, introduction of foreign genetic material using vectors, etc., can solve the problem of no reported application of hrf3 gene, etc., and achieve the effect of disease resistance enhancement

Inactive Publication Date: 2005-11-02
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report of hrf3 gene and its application in plant transgenic breeding engineering technology
At present, there is no report on the hrf gene transgenic breeding method with the hrf gene as the target gene

Method used

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  • Rice flavamonocell hrf3 gene, recombinant vector and breeding method for plant transgene
  • Rice flavamonocell hrf3 gene, recombinant vector and breeding method for plant transgene
  • Rice flavamonocell hrf3 gene, recombinant vector and breeding method for plant transgene

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Embodiment Construction

[0026] 1. The binary transformation of expressing hrf3 gene in plants—the construction of expression vector pBIH (pBI121::hrf3)

[0027] The plasmid used to construct the binary transformation-expression vector is transformed from T-DNA, except for the T-DNA 25bp repeat sequence (LB and RB), the promoter (P-nos) and terminator of the nopaline synthase gene In addition to T-nos, there are nptI used as a selection marker in prokaryotes (bacteria) and nptII used as a selection marker in eukaryotes (plants). The antibiotics used for selection are both Km and G- 418. Also in pBI121 are the promoter 35S (p35S) of the cauliflower mosaic virus expressed in eukaryotes and the uidA of the β-glucuronidase (Gus) gene. The hrf3 gene used as the target gene is inserted into the multi-enzyme cloning site between p35S and uid of the pBI121 plasmid ( FIG. 3 ), and the endonucleases used in the present invention are XbaI and BamHI. The binary vector pBI121 is a well-known public vector (Jeffe...

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Abstract

An hrf3 gene of rice Xanthomonas is disclosed. It can be used as the target gene to be constructed on the Ti-plasmid dual-element vector pBI121 expressed in plant, so obtaining its recombinant vector, pBI121:hrf3. Said recombinant vector is transferred to EHA105 to transform plant cells. The transformed regenerative plant (To generation) is screened in the culture medium and detected. The resultant transgenic plant has the improved broad-spectrum powder to resist diseases and pests and the improved other production characteristics.

Description

(1) Technical field [0001] The invention "rice xanthomonad hrf3 gene, recombinant vector and method for plant transgenic breeding" belongs to the field of biotechnology, and is specially used to improve plant disease resistance and other beneficial production traits through plant genetic engineering technology. (2) Technical background [0002] The gene hrf exists in two pathogenic varieties (pv.oryzae and pv.oryzicola) of rice Xanthomonas oryzae and other Xanthomonas spp., and belongs to hypersensitive response and pathogenicity (hypersensitive response and pathogenicity , hrp) gene cluster members. Most Gram-negative phytopathogenic bacteria have hrp gene clusters in which genes encoding proteins (Harpin) that induce allergic responses in plants vary. At present, the hrp gene clusters in four Gram-negative genera of plant pathogenic bacteria have been fully sequenced, and the four known hrp gene clusters have been classified according to the structure of the gene cluster,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00C12N5/10C12N15/31C12N15/52C12N15/63C12N15/82C12Q1/68
Inventor 王金生邵敏董汉松陈功友高学文李平杨春
Owner NANJING AGRICULTURAL UNIVERSITY
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